Diagnostic performance of Mycoplasmopsis bovis antibody ELISA tests on bulk tank milk from dairy herds

Abstract

Background Testing of bulk tank milk (BTM) for Mycoplasmopsis bovis (previously Mycoplasma bovis) antibodies is increasingly popular. However the performance of some commercially available tests is unknown, and cutoff values possibly need to be adjusted in light of the purpose. Therefore, the aim of this study was to compare the diagnostic performance of three commercially available M. bovis antibody ELISAs on BTM, and to explore optimal cutoff values for screening purposes. A prospective diagnostic test accuracy study was performed on 156 BTM samples from Belgian and Swiss dairy farms using Bayesian Latent Class Analysis. Samples were initially classified using manufacturer cutoff values, followed by generated values. Results Following the manufacturer’s guidelines, sensitivity of 91.4%, 25.6%, 69.2%, and specificity of 67.2%, 96.8%, 85.8% were observed for ID-screen, Bio K432, and Bio K302, respectively. Optimization of cutoffs resulted in a sensitivity of 89.0%, 82.0%, and 85.5%, and a specificity of 83.4%, 75.1%, 77.2%, respectively. Conclusions The ID-screen showed the highest diagnostic performance after optimization of cutoff values, and could be useful for screening. Both Bio-X tests may be of value for diagnostic or confirmation purposes due to their high specificity.

Individual- and Herd-Level Milk ELISA Test Status and Incidence for Paratuberculosis in Hubei Province, China

“…Not only with a high agreement with the serum ELISA, the milk ELISA has also been proven to have a good consistency with the MAP culture [34]. It is not only individual tests but also bulk tank milk that is widely used in cattle disease testing or even surveillance [30,31]. Although we did not evaluate the ability of bulk tank milk testing in this study, based on previous studies, it should have good application prospects in the detection of paratuberculosis.…”

“…It incorporates non-invasive methodologies that impose minimal stress on animals, thus providing a more ethical alternative. Milk antibody tests are widely used in the detection of many diseases, such as bovine tuberculosis [23], Q fever [30], Mycoplasmopsis bovis [31], and the bovine gammaherpesvirus 4 [32]. These findings coalesce to underscore the utility of a milk-based ELISA as a productive and viable alternative to serum-based assays for detecting MAP and bTB in dairy cattle.…”

Melatonin alleviates high-fat-diet-induced dry eye by regulating macrophage polarization via IFT27 and lowering ERK/JNK phosphorylation

“… 83 Like other immunoassays, it relies on the binding of an antibody to a target to facilitate detection. 84 , 85 The levels of inflammatory cytokines in the supernatant of cells were detected using ELISA kits according to the manual instructions. Shortly, the macrophage supernatant and gradient concentration of standards were added to the well and incubated for 2 h, followed by this addition of biotin antibody, horseradish peroxidase labeled streptavidin, the TMB and the termination solution.…”