Diagnostic performance of ID Screen® MVV-CAEV Indirect Screening ELISA in identifying small ruminant lentiviruses-infected goats

Abstract

Diagnostic performance of ID Screen ® MVV-CAEV Indirect Screening ELISA in identifying goats infected with small ruminant lentiviruses (SRLV) was evaluated. In total 299 serum samples from the collection of the Laboratory of Veterinary Epidemiology and Economics -109 truly positive and 190 truly negative -were used. To be enrolled in the study a serum sample had to come from at least 2 year-old goat which had reacted identically in two serological surveys preceding sample collection and was kept in a herd of stable serological status confirmed at least twice during preceding 5 years. Moreover, in seropositive herds at least 20% of goats had to be serologically positive at the moment when the serum sample was collected for the study. The test proved to have high accuracy. Area under curve was 98.8% (95% CI: 97.5%, 100%). Diagnostic performance of the test was almost identical (Youlden's index of 90%, sensitivity >90% and specificity >95%) within a fairly wide range of cut-off values -between 20% and 60%. At manufacturer's cut-off of 50% sensitivity and specificity were 91.7% (95% CI: 85.0%, 95.6%) and 98.9% (95% CI: 96.2%, 99.7%), respectively. For this cut-off positive likelihood ratio was 87 (95% CI: 22, 346) and negative likelihood ratio was 0.08 (95% CI: 0.04, 0.16). In conclusion, the results of this study indicate that ID Screen ® MVV-CAEV Indirect Screening ELISA is a highly accurate diagnostic test for SRLV infection.

Relationship between the dissemination of small ruminant lentivirus infection in goat herds and opinion of farmers on the occurrence of arthritis

“…Both ELISAs were performed according to manufacturers’ manuals. Sensitivity (Se) and specificity (Sp) was 98.6% and 99.3% respectively for Checkit ELISA [ 12 ], and 91.7% and 98.9%, respectively for ID Screen ELISA [ 13 ]. Apparent within-herd seroprevalence (AP) was the proportion of seropositive adult goats in the herd.…”

Seropositive bucks and within-herd prevalence of small ruminant lentivirus infection

“…To obtain true seroprevalence apparent seroprevalence was adjusted by the sensitivity and specificity of the ELISA test used. They were 84% and 100%, respectively, for both the Bommeli and Pourquier ELISAs [ 17 ], and 91.7% and 98.9%, respectively, for the ID Screen ELISA [ 18 ].…”

The effect of the subclinical small ruminant lentivirus infection of female goats on the growth of kids

“…Their results were interpreted according the cut-off points recommended by the manufacturers. At these cut-off points sensitivity and specificity of the three assays was as follows: 98.6% and 99.3% for Checkit ELISA [29], 98.8% and 97.2% for Pourquier ELISA [30], and 91.7% and 98.9% for ID Screen ELISA [31]. The change of ELISA kits during the study period resulted from their availability on the market and the financial policy of the laboratory responsible for serological testing.…”