Diagnostic methods in sepsis: the need of speed

Coelho, Fernando Rodrigues; Martins, Joilson O.

doi:10.1016/s0104-4230(12)70236-1

Cited by 21 publications

(12 citation statements)

References 71 publications

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“…On the other hand, procalcitonin (PCT) plays a pivotal role in the diagnosis of sepsis as well as in the prescription of effective medications. However, the mechanism of sepsis is complex, which renders PCT as a poor independent indicator for the differential diagnosis of infections . In recent years, studies have shown that heparin binding protein (HBP) can also be used as an indirect biomarker for the early diagnosis of sepsis, because it positively correlated with the PCT level .…”

Section: Introductionmentioning

confidence: 99%

Time‐resolved fluorescent immunoassay‐based combined detection of procalcitonin, C‐reactive protein, heparin binding protein, and serum amyloid A1 to improve the diagnostic accuracy of early infection

Niu

Liu

Zhu

et al. 2018

Clinical Laboratory Analysis

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Background Serological tests are indispensable in the diagnosis of early infection. At present, only procalcitonin (PCT) and C‐reactive protein (CRP) are commonly used in clinical practice. Recently, serum amyloid A1 (SAA1) and heparin binding protein (HBP) have been shown to be new biomarkers, because SAA1 is highly sensitive and specific for viral infections, and HBP is predictive for septic shock. In this study, PCT, CRP, HBP, and SAA1 were detected in different combinations to improve the diagnostic accuracy of early infection using the biotin‐avidin amplifying system‐based time‐resolved fluorescent immunoassay (BA‐TRFIA). Methods A time‐resolved fluorescent immunoassay for PCT, CRP, HBP, and SAA1 was developed and then tested in a clinical setting. All experiments were carried out using the DR6608 time‐resolved fluorescent immunoassay analyzer. Results The cutoff values of PCT, CRP, HBP, and SAA1 were 0.05 μg/L, 5.59 mg/L, 3.83 μg/L, and 1.56 mg/L, respectively. The area under the ROC curve (AUC) showed that PCT ˃ SAA1 ˃ CRP ˃ HBP > 0.8. A methodological comparison of the results showed that a combination of the four biomarkers had the highest accuracy for the diagnosis of infectious diseases. Conclusion The time‐resolved fluorescent immunoassay‐based combined detection of PCT, CRP, HBP, and SAA1 was shown to significantly improve the diagnostic accuracy of early infection. Thus, our results indicate that combined detection based on BA‐TRFIA may represent a promising strategy in the clinical diagnosis of infection.

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Section: Introductionmentioning

confidence: 99%

Time‐resolved fluorescent immunoassay‐based combined detection of procalcitonin, C‐reactive protein, heparin binding protein, and serum amyloid A1 to improve the diagnostic accuracy of early infection

Niu

Liu

Zhu

et al. 2018

Clinical Laboratory Analysis

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“…The onset of sepsis can occur with all its specific symptoms or, on the contrary, the clinical symptoms can be difficult to detect as they can be masked by underlying disease. Once there is a query of organ infection, one of the main goals is to identify the pathogen [5,6]. Typically, bacteriological diagnosis is made by cultures or Gram staining and microscopic identification.…”

Section: Introductionmentioning

confidence: 99%

Incidence of pathogens infections in a Romanian Intensive Care Unit and sensitivity to antibiotics. A prospective single center study

Bedreag

Rogobete

Luca

et al. 2016

Acta Medica Marisiensis

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Introduction: Nosocomial infections represent one of the biggest challenges faced by clinicians in the intensive care unit (ICU) and is associated with high morbidity and mortality. Infections in ICU are most often very serious and represent often the cause of hospitalization in intensive care clinics. Aim of the study: This paper presents the incidence of nosocomial infections, and the sensitivity to antibiotics encountered in our ICU. Material and Methods: This prospective study was conducted for two years at the Clinic of Anesthesia and Intensive Care, Emergency County Hospital “Pius Brinzeu” Timisoara, Romania. All patients admitted to the ICU were analyzed in terms of signs and symptoms of bacterial infections. Results: A total of 1081 microbiological reports were recorded. Among these, 635 (58.70 %) represented infections in the respiratory tract, 201 (18.60 %) in the bloodstream, 100 (9.30 %) in genitourinary tract, and 10 (0.90 %) in the central nervous system. The top five most frequently identified pathogen in microbiological reports are Klebsiella sp (17.60 %), Acinetobacter sp (14.20 %), Proteus mirabilis (13.80 %), Pseudomonas aeruginosa (12.90 %), Staphylococcus aureus - MSSA (12.80%). Conclusions: In order to choose empirical treatment, international guidelines should be consulted according to each pathology and adapted to the sensitivity encountered in the microbiology reports of the Critical Care Unit.

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“…54 If such a dipstick could be developed for a rapid urinary analysis, there are high-impact implications in diverse settings ranging from a busy ED to rural community-type settings with little access to laboratory or clinical analytical systems. 55…”

Section: Summary and Future Directionsmentioning

confidence: 99%

A Biomarker-Based Approach to Infectious Disease in the Pediatric Emergency Department

Aranke

Mian

2013

Clinical Pediatric Emergency Medicine

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Diagnostic methods in sepsis: the need of speed

Cited by 21 publications

References 71 publications

Time‐resolved fluorescent immunoassay‐based combined detection of procalcitonin, C‐reactive protein, heparin binding protein, and serum amyloid A1 to improve the diagnostic accuracy of early infection

Time‐resolved fluorescent immunoassay‐based combined detection of procalcitonin, C‐reactive protein, heparin binding protein, and serum amyloid A1 to improve the diagnostic accuracy of early infection

Incidence of pathogens infections in a Romanian Intensive Care Unit and sensitivity to antibiotics. A prospective single center study

A Biomarker-Based Approach to Infectious Disease in the Pediatric Emergency Department

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