Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains

Gerber, Priscilla F.; Lelli, Davide; Zhang, Jianqiang; Strandbygaard, Bertel; Moreno, Ana; Llombart‐Bosch, Antonio; Perulli, Simona; Bøtner, Anette; Comtet, Loïc; Roche, Mickael; Pourquier, Philippe; Wang, Chong; Opriessnig, Tanja

doi:10.1016/j.prevetmed.2016.11.005

Cited by 15 publications

(16 citation statements)

References 35 publications

(49 reference statements)

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“…As oppose to large, intense farming holdings, backyard holdings are characterized with lesser people/animal/vehicle circulation, known to be the major biosecurity risk for PEDV entrance and spread [30, 33]. Nevertheless, negative results must be interpreted with caution due to the abovementioned reasons coupled with well-known lower sensitivity of commercially available ELISA kits [34]. Despite the cross-reactivity between PEDV and TGEV that can take place when commercial Ab ELISA tests are used [35, 36], epidemiological evidence of a PEDV outbreak elaborated above [31] strongly suggest that the specificity of the Ab ELISA test used within the study frame was not an issue.…”

Section: Discussionmentioning

confidence: 99%

The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology

et al. 2019

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Background Porcine epidemic diarrhoea (PED) is an emergent/re-emergent viral pig disease (caused by the virus belonging to the Coronaviridae family, in specific the Alphacoronavirus genus) of global importance. Clinical presentation is characterized with acute diarrhoea, vomiting and dehydration in pigs of all ages, with a possible high mortality in suckling piglets. The disease emerged in the USA in 2013 causing heavy losses, and re-emerged in Europe in 2014, but with milder consequences. Results In the spring 2016, PED-like symptoms were reported to be seen on an agricultural holding in Eastern Croatia; laboratory workup confirmed the Croatia’s first PED outbreak ever. Porcine epidemic diarrhoea virus (PEDV) strain responsible for the outbreak was of the S-INDEL genotype, much the same as other European PEDV strains. In 2017, a post-outbreak serology was carried out in three counties in Eastern Croatia, revealing seropositivity in pigs bred on four large industrial holdings (9.09%). The seroprevalence across PEDV-positive holdings was up to 82.8%. The latter holdings were unanimously managed by an enterprise that had never reported PED before. Conclusions PED has emerged in Croatian pig population causing potentially considerable losses. The circulating strain was of the S-INDEL genotype. Serological workup proved PEDV spread to another four agricultural holdings, demonstrating the importance of not only external, but also internal biosecurity measures.

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Section: Discussionmentioning

confidence: 99%

The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology

et al. 2019

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“…However, we selected that cut-off value (60%) both easily applicable in routine activity and representing the optimal balance of Se (96.5) and Sp (97.69). The sensitivity of the test is therefore very high also in the case of infection due to the more recent PEDV strains belonging to S-INDEL group and it is likely not affected by genetic differences among circulating strains as already observed by Gerber et al (2016) who sustained the capacity of the cELISA to correctly identified pigs infected with G1a, G1b and G2b PEDV.…”

Section: Discussionmentioning

confidence: 78%

“…Additionally, the properties of this PEDV competitive ELISA and its use in field conditions have been analysed. In fact, it has been employed in parallel with a variety of "in-house" and commercial assays set up for the detection of anti-PEDV antibodies in two interlaboratory studies (Gerber et al, 2016;Strandbygaard et al, 2016) to test shared panels of pig sera collected both in the field and during experimental studies in some European and North American countries. As described in these two studies, our "in-house" competitive ELISA "performed very well and exhibited both high Se and Sp" (Se: 97-95% CI 90.0-100.0 Sp: 94-95% CI 83.0-100.0) and "had the overall highest detection" levels (Gerber et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV)

Sozzi

Moreno

Lelli

et al. 2018

Research in Veterinary Science

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A B S T R A C TPorcine epidemic diarrhoea virus (PEDV), belongs to the genus Alphacoronavirus in the family Coronaviridae and causes severe diarrhoea, vomiting, dehydration and high mortality in seronegative newborn piglets. Thus, a precise and rapid diagnosis of PEDV infection is important for the application of control measures to limit viral dissemination. In this investigation, a monoclonal antibodies (MAbs)-based competitive enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against PEDV was developed and validated. The diagnostic performance of the test was evaluated by receiver operating characteristic (ROC) analysis using a panel of 829 known sera collected from different commercial pig farms, with or without a history of PED presence and from experimentally infected pigs. The competitive ELISA showed excellent diagnostic performance and discriminatory power with high sensitivity (Se) and specificity (Sp) values (Se = 96.5%, 95% IC 94.1-98.1; Sp = 98.2%, 95% IC 96.3-99.3). Moreover, this competitive ELISA method has other properties, such as high feasibility of testing sera without pre-treatment and automatic and instrument-mediated revealing, that makes it appropriate for large-scale screenings of affected pig farms in endemic regions or for monitoring plans in PEDVfree areas.

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“…Porcine epidemic diarrhea virus (PEDV) is the causative agent of porcine epidemic diarrhea (PED), a highly contagious enteric disease characterized by severe watery diarrhea, vomiting and dehydration leading to enormous economic losses in the swine industry worldwide [1, 2]. After PEDV was first reported in Belgium in 1971 and the United Kingdom in 1978, it then emerged in other European countries over the following several years [3].…”

Section: Introductionmentioning

confidence: 99%

A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus

Wang

et al. 2019

J Nanobiotechnol

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Background Porcine epidemic diarrhea virus (PEDV), which is characterized by severe watery diarrhea, vomiting, dehydration and a high mortality rate in piglets, leads to enormous economic losses to the pork industry and remains a large challenge worldwide. Thus, a rapid and reliable method is required for epidemiological investigations and to evaluate the effect of immunization. However, the current diagnostic methods for PEDV are time-consuming and very expensive and rarely meet the requirements for clinical application. Nanobodies have been used in the clinic to overcome these problems because of the advantages of their easy expression and high level of stability. In the present work, a novel biotinylated nanobody-based blocking ELISA (bELISA) was developed to detect anti-PEDV antibodies in clinical pig serum. Results Using phage display technology and periplasmic extraction ELISA (PE-ELISA), anti-PEDV N protein nanobodies from three strains of PEDV were successfully isolated after three consecutive rounds of bio-panning from a high quality phage display VHH library. Then, purified Nb2-Avi-tag fusion protein was biotinylated in vitro. A novel bELISA was subsequently developed for the first time with biotinylated Nb2. The cutoff value for bELISA was 29.27%. One hundred and fifty clinical serum samples were tested by both newly developed bELISA and commercial kits. The sensitivity and specificity of bELISA were 100% and 93.18%, respectively, and the coincidence rate between the two methods was 94%. Conclusions In brief, bELISA is a rapid, low-cost, reliable and useful nanobody-based tool for the serological evaluation of current PEDV vaccines efficacy and indirect diagnosis of PEDV infection.

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Diagnostic evaluation of assays for detection of antibodies against porcine epidemic diarrhea virus (PEDV) in pigs exposed to different PEDV strains

Cited by 15 publications

References 35 publications

The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology

The emergence of porcine epidemic diarrhoea in Croatia: molecular characterization and serology

Development and validation of a monoclonal antibody-based competitive ELISA for detection of antibodies against porcine epidemic diarrhoea virus (PEDV)

A novel biotinylated nanobody-based blocking ELISA for the rapid and sensitive clinical detection of porcine epidemic diarrhea virus

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