2007
DOI: 10.2172/922093
|View full text |Cite
|
Sign up to set email alerts
|

Diagnostic evaluation of a multiplexed RT-PCR microsphere array assay for the detection of foot-and-mouth and look-alike disease viruses

Abstract: A high-throughput multiplexed assay (Multiplex Version 1.0) was developed for the differential laboratory diagnosis of foot-and-mouth disease virus (FMDV) from viruses which cause clinically similar diseases of livestock. This assay simultaneously screens for five RNA and two DNA viruses using multiplexed reverse transcription PCR (mRT-PCR) amplification coupled with a microsphere hybridization array and flow-cytometric detection. Two of the seventeen primer-probe sets included in this multiplex assay were ado… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1

Citation Types

0
1
0

Year Published

2012
2012
2012
2012

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(1 citation statement)
references
References 17 publications
0
1
0
Order By: Relevance
“…Figure 1 shows the geographic spread and specialization of the NAHLN, including APHIS’ National Veterinary Services Laboratories (NVSL) as the confirmatory and reference laboratories. Multiplex assays (Hindson et al., 2007) differentiating the exotic agent from endemics with similar clinical signs might be used to advantage in routine submission testing, if sensitivity were sufficient. Once a detection (particularly of a new agent or strain of agent) is made, analysis or evaluation may be needed before action.…”
Section: The Prevention Preparedness Response Recovery Continuummentioning
confidence: 99%
“…Figure 1 shows the geographic spread and specialization of the NAHLN, including APHIS’ National Veterinary Services Laboratories (NVSL) as the confirmatory and reference laboratories. Multiplex assays (Hindson et al., 2007) differentiating the exotic agent from endemics with similar clinical signs might be used to advantage in routine submission testing, if sensitivity were sufficient. Once a detection (particularly of a new agent or strain of agent) is made, analysis or evaluation may be needed before action.…”
Section: The Prevention Preparedness Response Recovery Continuummentioning
confidence: 99%