Diagnostic efficacy of biochemical markers in diagnosis post-mortem of ischaemic heart disease

Pérez-Cárceles, María D.; Noguera, José Antonio; Jiménez, José Luís; Martínez, P.; Luna, A.; Osuna, Eduardo

doi:10.1016/j.forsciint.2004.02.007

Cited by 73 publications

(41 citation statements)

References 31 publications

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“…Subsequently, molecules can be transported from the pericardial cavity to the peripheral circulation by the thoracic duct via the parietal pericardium, by the right lymphatic duct via the right pleural cavity, 5 or through the capillary network and the venous system 6, 7. Cardiac enzymes and troponins are present at higher levels in the PF than the peripheral blood, and increased levels of these molecules are used to aid the diagnosis of fatal myocardial infarct post-mortem 8, 9. Additionally, the PF contains biologically active factors and components, of possible myocardial origin, including atrial and brain natriuretic peptides and endothelin-1 10 .…”

Section: Introductionmentioning

confidence: 99%

Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis

et al. 2017

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The pericardial fluid (PF) is contained in the pericardial sac surrounding the heart. MicroRNA (miRNA) exchange via exosomes (endogenous nanoparticles) contributes to cell-to-cell communication. We investigated the hypotheses that the PF is enriched with miRNAs secreted by the heart and that it mediates vascular responses through exosome exchange of miRNAs. The study was developed using leftover material from aortic valve surgery. We found that in comparison with peripheral plasma, the PF contains exosomes enriched with miRNAs co-expressed in patients’ myocardium and vasculature. At a functional level, PF exosomes improved survival, proliferation, and networking of cultured endothelial cells (ECs) and restored the angiogenic capacity of ECs depleted (via Dicer silencing) of their endogenous miRNA content. Moreover, PF exosomes improved post-ischemic blood flow recovery and angiogenesis in mice. Mechanistically, (1) let-7b-5p is proangiogenic and inhibits its target gene, TGFBR1, in ECs; (2) PF exosomes transfer a functional let-7b-5p to ECs, thus reducing their TGFBR1 expression; and (3) let-7b-5p depletion in PF exosomes impairs the angiogenic response to these nanoparticles. Collectively, our data support the concept that PF exosomes orchestrate vascular repair via miRNA transfer.

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Section: Introductionmentioning

confidence: 99%

Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis

et al. 2017

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“…myocardial or skeletal muscle troponins, pulmonary surfactant-associated proteins (SPs), neuronal esterase and astroglia S100 protein, are used as biochemical and/or immunohistochemical markers for detecting the respective visceral injury [75][76][77][78][94][95][96][97][98][99][100][101][102][103][104][105][106]. Among them, proteins or polypeptides having a rapid turnover rate can be candidates for molecular biological analyses.…”

Section: Miscellaneousmentioning

confidence: 99%

“…Myocardial injury in the death process can be evaluated by the biochemical measurement of myocardial markers in blood and pericardial fluid [94][95][96][97][98][99][100]. In addition, previous studies indicated that topographical analyses of HIF1A, EPO and VEGF mRNA transcripts are useful to verify the site of ischemic myocardial injury; these mRNAs in the left ventricular wall were higher for acute myocardial infarction than for mechanical asphyxiation and drowning, providing a procedure to evaluate the respective severities of focal and diffuse myocardial injury due to ischemia/hypoxia [40].…”

Section: Circulatory Failure Including Shockmentioning

confidence: 99%

Forensic molecular pathology of violent deaths

Maeda

Zhu

Ishikawa

et al. 2010

Forensic Science International

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“…The area under the curves were calculated and analyzed by one-tailed test. The optimal compromise between sensitivity and specificity was determined graphically [31].…”

Section: Statistical Analysesmentioning

confidence: 99%

Increase in clusterin-containing follicles in the adenohypophysis of drug abusers

et al. 2006

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The hypothalamic-pituitary-adrenocortical (HPA) system in drug abusers may be affected due to disorders of the hypothalamic dopaminergic system. The present study investigated alterations in the adenohypophysis of middle-aged drug abusers (40-60 years of age), using clusterin-containing mixed cell-follicles as the indicator, in which clusterin (apolipoprotein J) is a multifunctional glycoprotein related to neurodegeneration. The paraffin-embedded adenohypophyses of methamphetamine and psychotropic drug abusers (n = 76) were compared with those of non-abusers (n = 82). The number of follicles was larger in drug abusers independent of the immediate cause of death, although the size was not significantly different. When cell types forming the follicles were immunohistochemically examined, drug abusers showed an increase of prolactin (PRL) cells and gonadotroph cells and a reciprocal decrease of growth hormone cells, suggesting hypofunction of dopaminergic neurons in the hypothalamus, while there was no change in the adrenocorticotropic hormone and thyroid-stimulating hormone cells. These increases of the clusterin-containing follicles and PRL cells in the follicles may be related to the dysfunction of dopaminergic neurons in the hypothalamus of chronic drug abusers and may be useful for investigating drug abuse in forensic casework.

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Diagnostic efficacy of biochemical markers in diagnosis post-mortem of ischaemic heart disease

Cited by 73 publications

References 31 publications

Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis

Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis

Forensic molecular pathology of violent deaths

Increase in clusterin-containing follicles in the adenohypophysis of drug abusers

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