Diagnostic Detection Systems Based on Gold Nanoparticle Probes

Storhoff, James J.; Marla, Sudhakar S.; Hagenow, Susan R.; Mehta, H. H.; Lucas, Adam; Viswanadham, G.; Patno, Tim; Buckingham, Wesley; Cork, William H.; Müller, Ulrich

doi:10.1117/12.476069

Cited by 23 publications

(33 citation statements)

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“…Several of the more common strategies for the detection of hybridization between a single-stranded oligonucleotide probe and a complementary DNA target sequence include those that involve the use of tags with characteristic properties (such as fluorescence, 1 color, [2][3][4] luminescence, 5 or radioactivity 6 ), gold colloids 4,[7][8] or gold colloids enhanced with silver metal [9][10][11] and/or microscopy, 12 surface-enhanced Raman spectroscopy, 13 surface plasmon resonance (SPR) spectroscopy, 14 electrochemistry, 15 quartz crystal microbalance, 16 scanometry, 17 digital cameras, 17 or light scattering. 18 However, only some of these techniques have been applied to the high-throughput screening of DNA microarrays.…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle Probes and Mid-Infrared Chemical Imaging for DNA Microarray Detection

et al. 2010

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To date most mid-infrared spectroscopic studies have been limited, due to lack of sensitivity, to the structural characterization of a single oligonucleotide probe immobilized over the entire surface of a gold-coated slide or other infrared substrate. By contrast, widely used and commercially available glass slides and a microarray spotter that prints approximately 120-μm-diameter DNA spots were employed in the present work. To our knowledge, mid-infrared chemical imaging (IRCI) in the external reflection mode has been applied in the present study for the first time to the detection of nanostructure-based DNA microarrays spotted on glass slides. Alkyl amine-modified oligonucleotide probes were immobilized on glass slides that had been prefunctionalized with succinimidyl ester groups. This molecular fluorophore-free method entailed the binding of gold-nanoparticle-streptavidin conjugates to biotinylated DNA targets. Hybridization was visualized by the silver enhancement of gold nanoparticles. The adlayer of silver, selectively bound only to hybridized spots in a microarray, formed the external reflective infrared substrate that was necessary for the detection of DNA hybridization by IRCI in the present proof-of-concept study. IRCI made it possible to discriminate between diffuse and specular external reflection modes. The promising qualitative results are presented herein, and the implications for quantitative determination of DNA microarrays are discussed.

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Section: Introductionmentioning

confidence: 99%

Nanoparticle Probes and Mid-Infrared Chemical Imaging for DNA Microarray Detection

et al. 2010

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“…Auf diese Weise gelang der Nachweis von Gold‐Nanopartikel‐markierten DNA‐Molekülen, die spezifisch an eine DNA‐funktionalisierte Oberfläche gebunden waren 248. Die Vergrößerung der Gold‐Nanopartikel durch katalytische Reduktion von Silberionen und die Abscheidung von metallischem Silber auf den Gold‐Nanopartikeln ergab eine 100fache Verstärkung des Signals 248a,d. Zwei unterschiedliche DNA‐Sequenzen konnten simultan nachgewiesen werden, wenn zwei Gold‐Nanopartikel unterschiedlicher Größe zur Markierung unterschiedlicher Oligonucleotide eingesetzt wurden 248c.…”

Section: Oberflächengebundene Biomolekül‐nanopartikel‐hybride In Dunclassified

“…In einem Experiment zur Detektion von Pestiziden mit Gold‐Nanopartikel‐markierten Antikörpern diente ein einfacher Flachbett‐Scanner dazu, Signale von der immunsensorischen Oberfläche auszulesen 251. In einem anderen Versuch wurde ein preiswerter Bildanalysator eingesetzt, um die von den evaneszenten Wellen induzierte Lichtstreuung an den silberverstärkten Gold‐Nanopartikel‐Markierungen zu erfassen 248d…”

Section: Oberflächengebundene Biomolekül‐nanopartikel‐hybride In Dunclassified

Integrierte Hybridsysteme aus Nanopartikeln und Biomolekülen: Synthese, Eigenschaften und Anwendungen

2004

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Metall‐ oder Halbleiter‐Nanopartikel und Nanostäbchen haben ähnliche Dimensionen wie Proteine (Enzyme, Antigene, Antikörper) oder DNA. Die Integration von Nanopartikeln (mit ihren besonderen elektronischen, photonischen und katalytischen Eigenschaften) mit Biomaterialien (mit ihren einzigartigen Erkennungsmechanismen sowie katalytischen und inhibierenden Fähigkeiten) ergibt hybride Nanobiomaterialien mit synergistischen Eigenschaften und Funktionen. Dieser Aufsatz beschreibt die aktuellsten Entwicklungen bei der Synthese von Hybridsystemen aus Biomolekülen und Nanopartikeln oder Nanostäbchen und deren Anwendung zum Aufbau von geordneten 2D‐ und 3D‐Strukturen in Lösung und auf Oberflächen. Schwerpunkte sind der Einsatz von Biomolekül‐Nanopartikel‐Konjugaten für die Bioanalytik sowie zur Herstellung bioelektronischer Funktionseinheiten und Nanoleiterbahnen aus Metall, Halbleitern oder magnetischen Materialien.

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“…Using unamplified bacterial genomic DNA, the detection of methicillin resistance in Staphylococci was recently demonstrated. 59 More importantly, the detection of specific single nucleotide polymorphisms (SNPs) in total human DNA without complexity reduction appears equally feasible through application of this technology. 60,61 CONCLUSIONS Considering these advances in array manufacture, assay development, miniaturization, and microfluidics, combined with nanoparticle-based ultrahigh sensitivity and specificity, the development of fully automated and even hand-held systems for genotyping and expression analysis at the point of care should not be far off.…”

Section: Approaches For Improvementsmentioning

confidence: 99%

Section: Definition Of Assay Sensitivity and Approaches For Improvementsmentioning

confidence: 99%

Evolution of High-Sensitivity Microarray Technology

Müller¹

2003

JALA: Journal of the Association for Laboratory Automation

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Nanosphere M icroarray technology is a multiplex analytical technique for the detection of many different analytes in a mixture of biomolecules. The detection limits for each of the analytes for which the array is designed depend on a multiplicity of reaction parameters, the array itself, and profoundly on the label and detection technology employed. Significant improvements in assay sensitivity have been achieved by optimizing all steps that affect the generation of signal and noise. Nanoparticle technology brings a new dimension to this technology by providing not only higher sensitivity but also improved specificity for hybridization-based microarray assay systems. ( JALA 2003;8:96-100)

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Diagnostic Detection Systems Based on Gold Nanoparticle Probes

Cited by 23 publications

References 0 publications

Nanoparticle Probes and Mid-Infrared Chemical Imaging for DNA Microarray Detection

Nanoparticle Probes and Mid-Infrared Chemical Imaging for DNA Microarray Detection

Integrierte Hybridsysteme aus Nanopartikeln und Biomolekülen: Synthese, Eigenschaften und Anwendungen

Evolution of High-Sensitivity Microarray Technology

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