Diagnostic deoxyribonucleic acid probes for infectious diseases.

Tenover, Fred C.

doi:10.1128/cmr.1.1.82-101.1988

Cited by 12 publications

(18 citation statements)

References 82 publications

(127 reference statements)

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“…Nucleic acid probes have become valuable tools in diagnostic microbiology (13). The distinct nucleotide base composition of P. gingivalis should facilitate the development of a specific DNA probe for diagnosis of the organism (1).…”

mentioning

confidence: 99%

Evaluation of a non‐radioactive DMA probe for detecting Porphyromonas gingivalis in subgingival specimens

Tay

Liu

Flynn

et al. 1992

Oral Microbiology and Immunology

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This study compared the ability of a nonradioactive digoxigenin-labeled DNA probe and anaerobic culture to identify subgingival Porphyromonas gingivalis. Total cellular DNA from P. gingivalis ATCC 33277T was labeled using the Genius kit from Boehringer Mannheim Biochemicals. Anaerobic culture was performed using VMGA III transport medium and enriched brucella blood agar. The DNA probe could detect as little as 1000 P. gingivalis cells added to supragingival plaque. Also, the probe could detect P. gingivalis when it was present in proportions too low to be visualized on overgrown bacterial plates. The probe showed no visible reaction with strains of various oral species or with thousands of non-P. gingivalis colonies from plaque samples. VMGA III could maintain the viability of P. gingivalis for up to 6 days, as evidenced by DNA probing of colony blot of subgingival cultures. A total cellular DNA probe for detecting P. gingivalis seems to offer a simple and reliable method of detecting the organism in subgingival specimens.

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mentioning

confidence: 99%

Evaluation of a non‐radioactive DMA probe for detecting Porphyromonas gingivalis in subgingival specimens

Tay

Liu

Flynn

et al. 1992

Oral Microbiology and Immunology

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“…Firstly, the classical hybridisation with a labelled DNA probe. In this technique, bacteria are applied to a solid phase, usually a nitrocellulose or nylon filter, After lysis of the bacteria the DNA is fixed to the filter and labelled DNA probes are added and allowed to react with their counterstrand, if present [5,6]. In this type of reaction, the DNA present in the sample is not multiplied.…”

Section: Nucleic Acid Hybridisation Technologymentioning

confidence: 99%

“…DNA probes, to be used for identification purposes may be derived from several sources. The most common way to obtain specific probes is the selection of random DNA fragments from a gene bank [6,13]. In this case, fragments are selected on the basis of the specificity that is desired for their application.…”

Section: Microbial Identificationmentioning

confidence: 99%

“…A second source of DNA probes for microbial identifications are specific virulence genes [6,16]. Genes coding for microbial virulence factors allow a direct answer to the question whether certain pathogenic types of bacteria are present in a specimen, without the need to detect non-virulent types of the same or related microorganisms.…”

Section: Microbial Identificationmentioning

confidence: 99%

“…The most important group of genes in this respect are the ribosomal RNA coding genes, especially the 16S rRNA genes in numerous bacteria. The use of the variable regions of the 16S rRNA as a source of specific microbial probes has been documented extensively [6,18,19]. A less well-known source of probes, especially for the Gram-negative bacteria, is given by the genes coding for the outer membrane proteins.…”

Section: Microbial Identificationmentioning

confidence: 99%

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Microbes in food processing technology

Hofstra

Vossen

Plas

1994

FEMS Microbiology Reviews

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There is an increasing understanding that the microbial quality of a certain food is the result of a chain of events. It is clear that the microbial safety of food can only be guaranteed when the overall processing, including the production of raw materials, distribution and handling by the consumer are taken into consideration. Therefore, the microbiological quality assurance of foods is not only a matter of control, but also of a careful design of the total process chain. Food industry has now generally adapted quality assurance systems and is implementing the Hazard Analysis Critical Control Point (HACCP) concept. Rapid microbiological monitoring systems should be used in these cases. There is a need for rapid and simple microbiological tests which can be adapted to the technology and logistics of specific production processes. Traditional microbiological methods generally do not meet these high requirements. This paper discusses the tests, based on molecular biological principles, to detect and identify microbes in food-processing chains. Tests based on DNA technology are discussed, including in vitro DNA amplification like the polymerase chain reaction (PCR) method and identifications based on RFLP, RAPD and DNA fingerprinting analysis. PCR-based methodology can be used for the rapid detection of microbes in food manufacturing environments. In addition, DNA fingerprinting methods are suitable for investigating sources and routes of microbial contamination in the food cycle.

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A biotinylated DNA probe to detect bacterial cells in artificially contaminated foodstuffs

Dovey

Towner

1989

Journal of Applied Bacteriology

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A biotin-labelled DNA probe was used in a dot-blot hybridization test to demonstrate the presence of Escherichia coli in a variety of artificially contaminated foodstuffs. Positive hybridization was detected by using a streptavidine/polyalkaline phosphatase conjugate to generate an insoluble coloured precipitate in the presence of an appropriate dye. The colour intensity was measured with a computer-controlled image analysis system which assessed objectively the hybridization signal produced by each sample. The method was capable of distinguishing positive hybridization at cell concentrations exceeding 10(4) cells/dot-blot, equivalent to 2 x 10(7) cells/g food, and had none of the drawbacks normally associated with the use of radioactively labelled DNA in hybridization techniques. The procedure is highly specific and takes less than 30 h. Many samples can be screened simultaneously and the procedure can be used to detect any species for which a suitable DNA probe is available.

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Diagnostic deoxyribonucleic acid probes for infectious diseases.

Cited by 12 publications

References 82 publications

Evaluation of a non‐radioactive DMA probe for detecting Porphyromonas gingivalis in subgingival specimens

Evaluation of a non‐radioactive DMA probe for detecting Porphyromonas gingivalis in subgingival specimens

Microbes in food processing technology

A biotinylated DNA probe to detect bacterial cells in artificially contaminated foodstuffs

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