Diagnostic Biomarker KIF23 is Associated With Immune Infiltration in Gastric Cancer

Abstract

Background: Kinesin family member 23 (KIF23), an index of tumor proliferation, can serve as a prognostic marker in numerous tumors. However, the relationship between KIF23 expression and immune infiltration and the diagnostic value of KIF23 remain unclear in GC (gastric cancer). This study aimed to explored the diagnostic value of KIF23 and its interactions with tumor-infiltrating immune cells in GC by bioinformatics analysis. Mothods: The relationship between clinicopathologic features and KIF23 expression was also analyzed using the Wilcoxon rank-sum test and logistic regression. And the expression level of KIF23 was validated by IHC and GEO databases, which was consistent with informatics results. Receiver operating characteristic (ROC) curves were generated to evaluate the value of KIF23 as a binary classifier using the area under the curve (AUC value).Results: High expression of KIF23 was significantly associated with longer overall survival and progression-free survival in GC. The mutations of KIF23 in GC were analyzed using cBioPortal and the Catalogue of Somatic Mutations in Cancer database. Enrichment analysis of co-expressed genes and KIF23 analysis was performed using LinkedOmics. By using STRING and GeneMANIA databases, we investigated the protein-coding genes related to KIF23 and its co-expression genes in GC tissues. Then, the relationship between KIF23 expression and immune infiltration in GC was investigated using Timer and GEPIA. We found that KIF23 might be used as a potential diagnostic biomarker in GC. Subsequently, KIF23 expression level was correlated with the infiltration levels of CD8 + T cells, macrophages, neutrophils, and more obviously with B cells and dendritic cells. In addition, KIF23 expression was significantly associated with T cell exhaustion (CTLA-4 and GZMB). KIF23 expression showed correlations with the infiltration of diverse immune markers in GC. Conclusions: Our findings suggest KIF23 can serve as a marker for immune infiltration and diagnostic in GC, making it a potential treatment of target.

Tumour‐associated macrophages in gastric cancer: From function and mechanism to application

“…Moreover, overexpressed MAT2A elevated the expression of RIP1 by increasing the H3K4 methylation at its promoter regions to induce the polarisation of M2 TAMs 47 . Several studies have demonstrated that the high expression of TUBA1A, ELK3, GKN2, COL1A2, RAI14, KIF23, GHRL, KCND2, SERPINE1, COL5A2, ALDOC and SLC38A2 and low expression of CD155 and AKR1B10 in GC indicate higher M2 TAM infiltration in the TME using data from public databases 93–107 . Furthermore, TAMs elevated the expression of PI3K‐γ by increasing the uptake of extracellular lipids, thereby inducing M2 phenotype polarisation 44 .…”