Diagnostic aptitude of West Nile virus-like particles expressed in insect cells

Rebollo, Belén; Sarraseca, Javier; Rodríguez, Ma José Rodríguez; Sanz, Antonio; Jiménez‐Clavero, Miguel Ángel; Venteo, Ángel

doi:10.1016/j.diagmicrobio.2018.02.003

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…VLPs can also be used as a diagnostic tool by their antigenic properties in the recognition of native virions. Co-expression of C, M and E structural proteins of WNV yielded noninfectious WNN VLPs, and the VLPs were shown to perform correctly in two different ELISAs for WNV diagnosis [96]. The potential use of VLPs as diagnostic tests for the above-mentioned common flaviviruses has also been reported [56].…”

Section: Diagnostic Assays Of Vlps For Flavivirus Detectionmentioning

confidence: 92%

“…Expression of WNV E protein without prM led to the proteolytic cleavage of the expressed E protein [55]. Co-expression of three WNV structural proteins in an insect cell expression system resulted in the assembly of VLPs in high yields [96]. Mutations in the prM protein (T20D, K31A, K31V, or K31T) reduced, or eliminated, the secretion of WNV VLPs, whereas they had less effect on wild-type WNV [97].…”

Section: Factors Affecting the Production Or Packaging Of Wnv Vlpsmentioning

confidence: 99%

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Recent Advances in the Development of Virus-Like Particle-Based Flavivirus Vaccines

Zhang

Jiang

et al. 2020

Vaccines

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Flaviviruses include several medically important viruses, such as Zika virus (ZIKV), Dengue virus (DENV), West Nile virus (WNV) and Japanese encephalitis virus (JEV). They have expanded in geographic distribution and refocused international attention in recent years. Vaccination is one of the most effective public health strategies for combating flavivirus infections. In this review, we summarized virus-like particle (VLP)-based vaccines against the above four mentioned flaviviruses. Potential strategies to improve the efficacy of VLP-based flavivirus vaccines were also illustrated. The applications of flavivirus VLPs as tools for viral detection and antiviral drug screening were finally proposed.

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Section: Diagnostic Assays Of Vlps For Flavivirus Detectionmentioning

confidence: 92%

Section: Factors Affecting the Production Or Packaging Of Wnv Vlpsmentioning

confidence: 99%

Recent Advances in the Development of Virus-Like Particle-Based Flavivirus Vaccines

Zhang

Jiang

et al. 2020

Vaccines

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“…Two recombinant baculovirus clones were generated to express, respectively, the NS1 protein and the structural proteins in the form of “virus-like particles” (VLP) of WNV, as previously described [ 8 , 9 ]. Briefly the NS1-coding region from NY99 034EDV “crow” strain was cloned into the baculovirus expression vector pAcHLA, expressed in Sf9 insect cells and purified by Ni-affinity column.…”

Section: Methodsmentioning

confidence: 99%

Monitoring Anti-NS1 Antibodies in West Nile Virus-Infected and Vaccinated Horses

Rebollo¹,

Sarraseca²,

Lecollinet

et al. 2018

BioMed Research International

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West Nile virus (WNV) is a zoonotic arboviral pathogen affecting humans, birds, and horses. Vaccines are available for veterinary use, which efficiently prevent the infection in horses. Most common diagnostic tools rely on the identification of the agent (RT-PCR, virus isolation), or on the detection of antibodies (IgM and IgG) recognizing structural proteins of the virus or neutralizing virus infection in cell cultures (virus-neutralization tests). The recent emergence of WNV in different parts of the world has resulted in an increase in the vaccination of horses in many countries. Methods for differentiation between infected and vaccinated animals (“DIVA” assays) would be useful for surveillance and control purposes but are still not available. A usual approach in this regard is the use of antibodies to nonstructural proteins as markers of nonvaccinated, infected animals, and the nonstructural NS1 protein of WNV has been proposed as a candidate for such a marker. The aim of this study was to test the hypothesis that NS1 can be a useful antigen in DIVA assays for differentiating WNV vaccinated and infected horses in field conditions. For that, we examined serum samples from either vaccinated and infected horses both from experimental infections/vaccinations (under controlled conditions) and from the field, exposed to natural infection or vaccinated in response to a risk of infection. The overall conclusion of the study is that NS1 antigen can effectively differentiate WNV infected from vaccinated horses in experimental (controlled) conditions, but this differentiation might be difficult depending on the conditions prevailing in the field.

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“…Mosquito cell-derived dengue 2 VLPs produced from stable C6/36 cell clones have been characterized in immunogenicity [35]. However, an understanding of VLP structures requires a characterization in the presence of epitopes and an estimate of their resemblance to authentic virions [36][37][38][39][40]. Researchers have demonstrated the efficacy of baculovirus as a vector for gene delivery into mosquitoes [41].…”

Section: Introductionmentioning

confidence: 99%

Mosquito Cell-Derived Japanese Encephalitis Virus-Like Particles Induce Specific Humoral and Cellular Immune Responses in Mice

Chang

Chiao

Hsu

et al. 2020

Viruses

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The Japanese encephalitis virus (JEV) is the major cause of an acute encephalitis syndrome in many Asian countries, despite the fact that an effective vaccine has been developed. Virus-like particles (VLPs) are self-assembled multi-subunit protein structures which possess specific epitope antigenicities related to corresponding native viruses. These properties mean that VLPs are considered safe antigens that can be used in clinical applications. In this study, we developed a novel baculovirus/mosquito (BacMos) expression system which potentially enables the scalable production of JEV genotype III (GIII) VLPs (which are secreted from mosquito cells). The mosquito-cell-derived JEV VLPs comprised 30-nm spherical particles as well as precursor membrane protein (prM) and envelope (E) proteins with densities that ranged from 30% to 55% across a sucrose gradient. We used IgM antibody-capture enzyme-linked immunosorbent assays to assess the resemblance between VLPs and authentic virions and thereby characterized the epitope specific antigenicity of VLPs. VLP immunization was found to elicit a specific immune response toward a balanced IgG2a/IgG1 ratio. This response effectively neutralized both JEV GI and GIII and elicited a mixed Th1/Th2 response in mice. This study supports the development of mosquito cell-derived JEV VLPs to serve as candidate vaccines against JEV.

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Diagnostic aptitude of West Nile virus-like particles expressed in insect cells

Cited by 8 publications

References 25 publications

Recent Advances in the Development of Virus-Like Particle-Based Flavivirus Vaccines

Recent Advances in the Development of Virus-Like Particle-Based Flavivirus Vaccines

Monitoring Anti-NS1 Antibodies in West Nile Virus-Infected and Vaccinated Horses

Mosquito Cell-Derived Japanese Encephalitis Virus-Like Particles Induce Specific Humoral and Cellular Immune Responses in Mice

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