Diagnostic application of total antioxidant capacity in seminal plasma to assess oxidative stress in male factor infertility

Roychoudhury, Shubhadeep; Sharma, Rakesh; Sikka, Suresh C.; Agarwal, Ashok

doi:10.1007/s10815-016-0677-5

Cited by 73 publications

(66 citation statements)

References 57 publications

(61 reference statements)

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“…Our laboratory standardized a colorimetric TAC assay in a kit form that was used successfully with seminal plasma [Mahfouz et al 2009]. In a recent study, we established a new diagnostic cutoff TAC value of 1947 µm in seminal plasma with 63.0% specificity and 59.5% sensitivity to distinguish the prevalence of oxidative stress in infertile patients compared with healthy men [Roychoudhury et al 2016]. At this cutoff value the sensitivity was 59.5% and the specificity was 63.0%.…”

Section: Measurement Of Seminal Oxidative Stressmentioning

confidence: 99%

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Agarwal

Roychoudhury

Bjugstad³

et al. 2016

Therapeutic Advances in Urology

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128

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The diagnosis of male infertility relies largely on conventional semen analysis, and its interpretation has a profound influence on subsequent management of patients. Despite poor correlation between conventional semen parameters and male fertility potential, inclusion of advanced semen quality tests to routine male infertility workup algorithms has not been widely accepted. Oxidative stress is one of the major mediators in various etiologies of male infertility; it has deleterious effects on spermatozoa, including DNA damage. Alleviation of oxidative stress constitutes a potential treatment strategy for male infertility. Measurement of seminal oxidative stress is of crucial role in the identification and monitoring of patients who may benefit from treatments. Various tests including reactive oxygen species (ROS) assay, total antioxidant capacity (TAC) assay or malondialdehyde (MDA) assay used by different laboratories have their own drawbacks. Oxidation-reduction potential (ORP) is a measure of overall balance between oxidants and antioxidants, providing a comprehensive measure of oxidative stress. The MiOXSYS™ System is a novel technology based on a galvanostatic measure of electrons; it presents static ORP (sORP) measures with static referring to the passive or current state of activity between oxidants and antioxidants. Preliminary studies have correlated sORP to poor semen qualities. It is potentially useful in prognostication of assisted reproductive techniques outcomes, screening of antioxidants either in vivo or during IVF cycles, identification of infertile men who may benefit from treatment of oxidative stress, and monitoring of treatment success. The simplified laboratory test requiring a small amount of semen would facilitate clinical application and research in the field. In this paper, we discuss the measurement of ORP by the MiOXSYS System as a real-time assessment of seminal oxidative stress, and argue that it is a potential valuable clinical test that should be incorporated into the male infertility workup and become an important guide to the treatment of oxidative stress-induced male infertility.

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Section: Measurement Of Seminal Oxidative Stressmentioning

confidence: 99%

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Agarwal

Roychoudhury

Bjugstad³

et al. 2016

Therapeutic Advances in Urology

Self Cite

128

118

View full text Add to dashboard Cite

show abstract

“…High levels of ROS are found in 25%–40% of infertile men and in 40%–80% of infertile patients with spinal cord injury (de Lamirande, Leduc, Iwasaki, Hassouna, & Gagnon, ; Sharma & Agarwal, ). In addition, infertile patients also have low levels of antioxidants in their seminal plasma (Ko, Sabanegh, & Agarwal, ; Roychoudhury, Sharma, Sikka, & Agarwal, ; Sharma, Pasqualotto, Nelson, Thomas, & Agarwal, ). Yet, for successful fertilisation, a balance between oxidants and available antioxidants is essential for normal chromatin compaction in maturing spermatozoa during epididymal transit, capacitation, hyperactivation, acrosome reaction and sperm–oocyte fusion (Henkel et al., ; Ko et al., ; Sharma & Agarwal, ).…”

Section: Introductionmentioning

confidence: 99%

Determination of seminal oxidation-reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility

et al. 2017

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Oxidative stress (OS) is an important contributing factor to male infertility. While previous methods to measure seminal OS are time-consuming and limited to the use of freshly produced semen, oxidation reduction potential (ORP) is easier and quicker to perform and can also be used in frozen semen. Therefore, this study evaluated the clinical utility of ORP as a potential marker of male infertility. ORP was measured in semen samples from 293 patients and 15 fertile controls and categorised according to WHO criteria as normozoospermic, oligozoospermic, asthenozoospermic, teratozoospermic and oligoasthenoteratozoospermic. Receiver operating characteristic (ROC) curves were generated to differentiate these categories. Semen parameters were significantly different when subjects were grouped as control and patients or between the patient and normozoospermic group for concentration and morphology. ORP levels were significantly different between the control and normozoospermic group. When subjects were grouped based on concentration, motility, morphology or a combination of these, the area under the ROC curve, sensitivity, specificity, positive predictive value and cut-off values were significantly different. These differences were significant when combined with ORP and grouped with any two sperm abnormalities. In conclusion, ORP is a quick, easy, cost-effective and reliable marker of semen quality as well as oxidative stress for use in a clinical setting.

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“…We can explain this only from a theoretical point of view, given spermatozoa have no mechanism to repair DNA damage produced by oxidative stress. Sperm use oxidative phosphorylation from mitochondria located in the middle part and Glycolysis in the head and the main part for the movement of flagella and energy production, and at the same time, these processes generate reactive oxygen species (ROS), thus contributing to the formation of oxidative stress and direct injury to sperm's DNA [8]. There is evidence that increased ROS formation happens in sperm with excess of cytoplasm around the middle part, resulting in dysfunction in sperm's motility and abnormal morphology that impact negatively on the potential of fertilization [9].…”

Section: Discussionmentioning

confidence: 99%

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

Sánchez¹

2017

OGIJ

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The semen volume is made up by the accessory glands (90%) and germinal cells (10%) (RB Mayer, 2015). Seminal plasma has enabling factors for sperm microenvironment (Aida Pujol, 2016), allowing among many other processes, sperm maturation (Yu-Wen Kuo, 2016).Objective: To analyze the correlation of seminal volume parameters with the outcome of fertilized oocytes. Material and methods:It is a prospective observational study, including 100 couples undergoing in vitro fertilization. The seminal sample was obtained by ejaculation and analyzed with WHO's 2010 criteria (Andrology laboratory procedures manual, 2010). Data encompassed seminal volumes and fertilized oocytes. Statistical analysis was made with Kolmogorov-Smirnov normalization, determining correlation of Pearson and significance of p ≤0.05. Results:The following parameters were registered: Seminal volume: 1.5-7.6 (average 2.7 ml), number of fertilized MII oocytes: 1-33 (average 10 oocytes). Pearson correlation of 0.737 (p= 0.002). By dividing groups of fertilized oocytes and seminal volumes by standard Kolmogorov we obtained significance with the number of fertilized eggs with 4.8 and 14 oocytes (p=0.001), and we also obtained significance with the seminal volume, ranging from 2 to 3.2ml (p=0.003).Discussion: Our study shows that it exists a prognostic relationship between the seminal volume and the number of fertilized eggs in an in vitro fertilization. A seminal volume ranging from 2 to 3.2ml has a better chance to produce adequate fertilization. Volumes lower than this values may reflect inadequate sperm formation and regulation and volumes greater may reflect other inflammatory conditions, such as infection (Jared M Bienek, 2016). Conclusion:Seminal volume reflects a healthy testicular function, producing spermatozoa capable of achieving good fertilization. Alterations on the seminal volume indicate inadequate conditions for the correct formation of spermatozoa, or increased inflammation molecules that damage the microenvironment.

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Diagnostic application of total antioxidant capacity in seminal plasma to assess oxidative stress in male factor infertility

Cited by 73 publications

References 57 publications

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Oxidation-reduction potential of semen: what is its role in the treatment of male infertility?

Determination of seminal oxidation-reduction potential (ORP) as an easy and cost-effective clinical marker of male infertility

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

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