Diagnostic application of monoclonal antibody KB90 (CD11c) in acute myeloid leukaemia

Master, Peter S.; Richards, Stephen J.; Kendall, Jane C.; Roberts, B. E.; Scott, C S

doi:10.1007/bf00320851

Cited by 8 publications

(5 citation statements)

References 10 publications

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“…Firstly, and in conkmation of previous investigations (1-3), it is evident that CD 14 expression when present is diagnostically indicative of a monocytic AML subtype. Additionally, the results obtained by two-colour analysis show that membrane C D l l c and CD14 expression are independent indicators of monocytic involvement and lend support to a previous contention that CD 1 l c should be used as a "second line" reagent in CD 14 -acute leukaemias where morphology and cytochemistry suggests a diagnosis of M4 or M5 (5). However, whilst the relative value of these two reagents is apparent, the finding that 5/15 morphologically and cytochemically (ANAE + ) defined M4/M5 cases were both C D l l c -and CD14emphasises that the diagnostic subdivision of AML should not be based on immunophenotyping alone.…”

Section: Discussionsupporting

confidence: 80%

Flow cytometric analysis of membrane CD11b, CD11c and CD14 expression in acute myeloid leukaemia: relationships with monocytic subtypes and the concept of relative antigen expression

Scott¹,

Richards²,

Master³

et al. 1990

European J of Haematology

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Blast cells from 26 cases of acute myeloid leukaemia (AML) were examined, by single and “two‐colour” flow cytometry, for relationships between membrane CD11b (monoclonal antibody OKM1), CD11c (KB90) and CD14 (Leu‐M3). Increased expression of all three determinants was associated with myelomonocytic leukaemias, with their relative diagnostic value in discriminating monocytic (M4 and M5) from non‐monocytic (M1, M2 and M3) subtypes being CD14 > CD11c > CD11b. However, the results also indicated, because of the heterogenous expression of CD11c in particular, and to a lesser extent CD11b, that the patterns or histograms of fluorescent staining were potentially more informative than an empirical subdivision of blasts into positive and negative sub‐populations. In addition, analysis of phenotypic correlations by simultaneous two‐colour fluorescence showed that the expression of CD11b and CD11c determinants by leukaemic myeloid blasts was highly correlated, in contrast to the expression of CD14 and CD11c which were relatively independent. Consequently, CD11c + myeloid blasts almost always co‐expressed CD11b whereas CD14 + cases of AML often comprised CD14 + CD11c + and CD14 + CD11c‐ subpopulations. It is concluded from these observations that CD11c immunophenotyping is a useful supplementary investigation, particularly in CD14‐ cases of myelomonocytic leukaemia. However, it is also apparent that the presence of membrane CD11c per se is not lineage‐specific and that the level of expression is perhaps a more discriminatory factor.

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Section: Discussionsupporting

confidence: 80%

Flow cytometric analysis of membrane CD11b, CD11c and CD14 expression in acute myeloid leukaemia: relationships with monocytic subtypes and the concept of relative antigen expression

Scott¹,

Richards²,

Master³

et al. 1990

European J of Haematology

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“…We employed CD4, 4 , 10 CD11c, 10 , 12 , 16 CD14, 5-11 , 13-16 and CD64 5-15 antigens as monocyte-related antigens, and diagnosed a patient with AML-M5 when the leukemic cells expressed at least one of these antigens at the level of 10% in the present study. Of these antigens, CD4, CD14, and CD64 may have been established as monocyte-related antigens.…”

Section: Discussionmentioning

confidence: 99%

“…Of these antigens, CD4, CD14, and CD64 may have been established as monocyte-related antigens. 4-16 CD11c is expressed on immature monocytes 10 , 12 , 16 like CD4. CD11c is also expressed on granulocytes, but mainly on mature granulocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Surface antigens that indicate leukemic cells to be of a monocytic nature include CD4, CD11c, CD14, and CD64. Immature and mature monocytes express CD64, 5-15 CD4, 4 , 10 and CD11c 10 , 12 , 16 , antigens on their surface membranes, whereas CD14 5-11 , 13-16 is mostly expressed on mature monocytes. However, all of these antigens are not simultaneously expressed on leukemic cells in a single AML-M5 case; sole or dual expression of these antigens appears to be common.…”

Section: Introductionmentioning

confidence: 99%

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Acute monocytic leukemia diagnosed by flow cytometry includes acute myeloid leukemias with weakly or faintly positive non-specific esterase staining

et al. 2018

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A diagnosis of acute monocytic leukemia (AML-M5) based on α-naphthyl butyrate esterase (α-NB) staining has some problems, because AML-M5 leukemic cells often show weak or faint positivity on α-NB staining. In these situations, some cases of AML-M5 tend to be misdiagnosed as AML-M0. Therefore, we evaluated the significance of weak or faint α-NB staining in AML-M5 diagnosed by flow cytometry (FCM). Nineteen AML cases in which leukemic cells were negative for naphthol AS-D chloroacetate esterase staining were studied. For FCM, we defined leukemic cells as having a monocytic nature when more than 10% of the leukemic cells were positive for at least one of the following antigens: CD4, CD11c, CD14, and CD64. The monocytic nature determined by FCM was consistent with positive or weak positivity on α-NB staining. Five of 6 cases in which leukemic cells exhibited faint positivity for α-NB staining could be diagnosed as AML-M5 by FCM, while negative α-NB staining was consistent with a diagnosis of AML-M0. These results suggest that AML-M5 should be taken into consideration even when leukemic cells are faintly positive for α-NB staining.

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“…In addition, it was not surprising to find genes of cell lineage markers such as CD33 or CD11c. These lineage markers belong to a family of proteins used to distinguish the cell lineage of leukemic proliferating blasts by immunophenotyping techniques (2)(3)(4). The presence of these lineage markers corresponds to a stage of cell differentiation.…”

mentioning

confidence: 99%

Differentiation of Acute Myeloid Leukemia from B- and T-Lineage Acute Lymphoid Leukemias by Real-Time Quantitative Reverse Transcription-PCR of Lineage Marker mRNAs

et al. 2004

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Results: Three patterns of expression were detected. In the first, CD19, CD79a, and MPO mRNAs were less abundant than CD3e. In the second pattern, MPO mRNA was more abundant than the other three mRNAs. In the third, CD19 or CD79a was more highly expressed than CD3e and MPO. The three patterns corresponded to T-ALL, AML, and B-ALL, respectively. The use of cutoffs to establish qualitatively the pattern of coexpression of the four lineage markers provided the same

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Diagnostic application of monoclonal antibody KB90 (CD11c) in acute myeloid leukaemia

Cited by 8 publications

References 10 publications

Flow cytometric analysis of membrane CD11b, CD11c and CD14 expression in acute myeloid leukaemia: relationships with monocytic subtypes and the concept of relative antigen expression

Flow cytometric analysis of membrane CD11b, CD11c and CD14 expression in acute myeloid leukaemia: relationships with monocytic subtypes and the concept of relative antigen expression

Acute monocytic leukemia diagnosed by flow cytometry includes acute myeloid leukemias with weakly or faintly positive non-specific esterase staining

Differentiation of Acute Myeloid Leukemia from B- and T-Lineage Acute Lymphoid Leukemias by Real-Time Quantitative Reverse Transcription-PCR of Lineage Marker mRNAs

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