Diagnostic Application of Monoclonal Antibodies to Intermediate Filaments<sup>a</sup>

Virtanen, Ismo; Miettinen, Markku; Lehto, Veli‐Pekka; Kariniemi, Arja‐Leena; Paasivuo, R

doi:10.1111/j.1749-6632.1985.tb50441.x

Cited by 153 publications

(53 citation statements)

References 40 publications

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“…In pituitary, double stainings with antibodies against COMT and neurophysin (rabbit antiserum; Dakopatts), luteinizing hormone (rabbit anti-LH; a kind gift of Dr. A. F. Parlow) were performed as described earlier (Panula and Lindberg, 1987). In hypothalamus, double staining of the sections with antibodies against COMT and glial fibrillar acidic protein (rabbit anti-GFAP Dakopatts) [specific for glial cells and a mouse monoclonal neurofilament (NF)]; (Virtanen et al, 1985) antibody, specific for neurons, was carried out. Immunohistochemistry was performed as described above but replacing the primary and secondary antibodies as follows.…”

Section: Methodsmentioning

confidence: 99%

Distribution of catechol-O-methyltransferase enzyme in rat tissues.

Karhunen

Tilgmann

Ulmanen

et al. 1994

J Histochem Cytochem.

108

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(4A3288).In the present study we show the distribution of catechol-0-methyltransferase (COMT) in various rat tissues with a highly specifk antiserum prepared against recombinant rat COMT. Immunoprecipitation and immunocytochemical controls confiied the COMT-specificity of the antibodies. The antiserum detected both the 24 w soluble and the 28 KD membrane-bound forms of the enzyme. By immunohistochemical staining the COMT enzyme was found in most rat tissues. Staining was most intense in the liver and in the kidney, in agreement with pmious studies and our immunoblotting results. In the gastrointestinal tract, epithelial cells of the stomach, duodenum, and ileum were immunoreactive for COMT. In panaeas, COMT immunoreactivity was found in insulin-producing p-cells and somatostatin-

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Section: Methodsmentioning

confidence: 99%

Distribution of catechol-O-methyltransferase enzyme in rat tissues.

Karhunen

Tilgmann

Ulmanen

et al. 1994

J Histochem Cytochem.

108

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“…We have recently raised and characterized a mouse MAb that detects the human Ln ␤ 1 chain in cryostat tissue sections as well as in Western blots (LnB1; Virtanen et al, submitted for publication). Anti-cytokeratin 19 serum (Leoncini et al 1990) and the MAb 2A4 recognizing cytokeratins 8, 18, and 19 (Virtanen et al 1985) were used to identify trophoblastic cells in double immunostainings and serial sections, respectively. The MAb Ki-67 was used to identify proliferating cells (Gerdes et al 1983).…”

Section: Antibodiesmentioning

confidence: 99%

The Distribution of Laminins and Fibronectins Is Modulated During Extravillous Trophoblastic Cell Differentiation and Decidual Cell Response to Invasion in the Human Placenta

Korhonen

Virtanen²

1997

J Histochem Cytochem.

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SUMMARYWe studied the distribution of laminin (Ln) ␣ 1-␣ 3, ␤ 1-␤ 3, and ␥ 1 chains, and of the extradomain-A (EDA) and EDB and the oncofetal epitope of fibronectin (Onc-Fn) in extravillous trophoblastic cells and decidua in the human placenta by immunohistochemistry. We found that the transition from villous to extravillous trophoblast was accompanied by emergence of immunoreactivity for EDA-, EDB-, and Onc-Fn among the cells. Furthermore, whereas the villous trophoblastic basement membrane (BM) contains Ln ␣ 1, ␣ 2, ␤ 1, ␤ 2, and ␥ 1 chains, immunoreactivity for Ln ␣ 1, ␤ 1, and ␥ 1, but not for Ln ␣ 2 and ␤ 2 chains, was detected in association with extravillous trophoblastic cells. Interestingly, although immunoreactivity for the Ln ␣ 1, ␣ 2, ␤ 1, ␤ 2, and ␥ 1 chains was detected in all decidual cell BMs, EDB-Fn and Onc-Fn were detected only in decidua that had been invaded by the trophoblast. In summary, our results describe distinct changes in the distribution of Ln and Fn isoforms during the differentiation of villous trophoblast into extravillous trophoblastic cells. Furthermore, EDB-and Onc-Fn are preferentially found in decidua that has been invaded by the trophoblast, indicating that the deposition of these Fn isoforms reflects a decidual cell response to invasion. (J Histochem Cytochem 45:569-581, 1997)

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“…Keratinous microcysts did not react with cither 35 [SHI1 or PKK3, whereas the cords and nests, and ductal structures were positive for both antibodies. These two antibodies arc known to recognize cytokeratins expressed in simple epithclia [19,20] but not in stratified squamous epithclia. This finding appears to be consistent with our speculation that keratinous microcysts tend to dif ferentiate toward hair follicles, whereas the epithelial cords and nests, and ductal structures differentiate toward eccrinc sweat glands.…”

Section: Discussionmentioning

confidence: 99%