Diagnostic and therapeutic research on ultrasound microbubble/nanobubble contrast agents (Review)

Ma, Jing; Xu, Chi; Gao, Feng; Chen, Ming; Fan, Liangqian; Du, Lian Fang

doi:10.3892/mmr.2015.3941

Cited by 35 publications

(20 citation statements)

References 54 publications

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“…More and more therapeutic applications of microbubbles are emerging at present [ 9 , 12 – 14 ]. This novel ultrasound, known as microbubble-mediated ultrasound (MUS), has the ability to produce greater microstream and microjet than LIPUS [ 15 , 16 ]. To our knowledge, whether MUS has more effect on the repair progress in steroid-associated osteonecrosis is unknown.…”

Section: Introductionmentioning

confidence: 99%

Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

Yan

et al. 2018

BioMed Research International

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Microbubbles magnify the acoustic pressure of low-intensity pulsed ultrasound (LIPUS) and may enhance its bioeffect for diagnostic and therapeutic purposes. This study compared the effect of this novel microbubble-mediated ultrasound (MUS) with that of the traditional LIPUS on osteogenesis and neovascularization in a rabbit model of steroid-associated osteonecrosis. We hypothesized that MUS might outweigh LIPUS on promoting osteogenesis and neovascularization in steroid-associated osteonecrosis. The bilateral femoral head necrosis was induced by lipopolysaccharide and methylprednisolone in the rabbits. The indices of bone mineral density (BMD), trabecular number, maximal loading strength, and mineral apposition rate were analyzed, demonstrating that the animal model of steroid-associated osteonecrosis was successfully established. Both the MUS group (GM) and the LIPUS group (GL) were insonated 20 min daily for six weeks. GM received an extra intracapsular injection of microbubbles before insonation every other day. Fluorescence bone labeling, Micro-CT Analysis, biomechanical test, quantitative real-time PCR, Western blot analysis, and histological evaluation were performed for comparing GM with GL. The results demonstrated a 39% higher mineral apposition rate in GM compared with GL. The BMD and the maximal loading strength of femoral head of GM increased by 4.3% and 27.8% compared to those of GL, respectively. The mRNA and protein expression of BMP-2 and VEGF were also significantly higher in GM. The number of blood vessels of GM was 65% greater than that of GL. MUS is more potent than LIPUS in enhancing osteogenesis, neovascularization, and biomechanical strength of femoral head in the animal model of steroid-associated osteonecrosis. Without increasing the intensity of insonation or the risk of tissue damage, MUS is better for inhibiting the process of steroid-associated osteonecrosis.

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Section: Introductionmentioning

confidence: 99%

Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

Yan

et al. 2018

BioMed Research International

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“…These are generally coated with albumin or lipoproteins for stability in blood 11 . While the majority of microbubble work has focused on contrast agents, studies evaluating delivery of therapeutics or combining therapeutics with contrast is growing rapidly 12 .…”

mentioning

confidence: 99%

On-Demand and Long-Term Drug Delivery from Degradable Nanocapsules

Menikheim¹,

Leckron²,

Bernstein

et al. 2020

ACS Appl. Bio Mater.

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While there are a number of nanomaterials that can lead to on demand drug delivery or longterm delivery, there are limited nanotechnologies that are stable for long times, deliver sustained amounts of drugs, and achieve repeated, on demand delivery. We have developed a system based on polyurethane nanocapsules as a platform for long-term and on demand delivery. We synthesized nanocapsules encapsulating either a model drug, fluorescein, or a clinically relevant drug, acriflavine, a HIF-1alpha inhibitor. The nanocapsules were ~250 nm in diameter as measured by DLS and confirmed via SEM, and the molecules were localized in the walls of the nanocapsules as determined by confocal microscopy. Release studies were performed at 37 C in PBS, and both the fluorescein and acriflavine were delivered over several weeks. At the end of the release, no pellet was detectable upon centrifugation of the nanocapsules confirming degradation of the polyurethane shells. The same particles released a fraction of their payload upon the application of either an ultrasonic probe or a clinical grade, ultrasound imaging system used for assessing the retina. The amount of drug released could be tailored by the energy applied to the nanocapsules. One of the most exciting findings beyond being able to tailor how much was released based on the energy applied and the time it was applied was that these nanocapsules could be triggered to release multiple times with at least 10 separate release events triggered for each formulation. Being able to tailor the on-demand release over multiple cycles has the potential to fundamentally change how we can approach delivery of drugs for a variety of applications.

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“…An interesting application of nanotechnology for the PDT-guided treatment of PC involves the use of microbubbles. Microbubbles (MBs) are gas-filled structures stabilized by an external lipid layer, that are used as contrast agents for ultrasound (US) mediated clinical diagnosis (Ma et al, 2015; Dimcevski et al, 2016). In addition, they might represent promising platforms for cancer treatment by virtue of their responsiveness to US irradiation which is called Ultrasound Targeted Microbubble Destruction (UTMD), that enables the drug release through the cavitation effect (Lentacker et al, 2014).…”

Section: Nanoparticles-mediated Pdt Of Prostate Cancermentioning

confidence: 99%

Light-Induced Therapies for Prostate Cancer Treatment

et al. 2019

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Prostate cancer (PC) is one of the most widespread tumors affecting the urinary system and the fifth-leading cause from cancer death in men worldwide. Despite PC mortality rates have been decreasing during the last years, most likely due to an intensification of early diagnosis, still more than 300,000 men die each year because of this disease. In this view, researchers in all countries are engaged in finding new ways to tackle PC, including the design and synthesis of novel molecular and macromolecular entities able to challenge different PC biological targets, while limiting the extent of unwanted side effects that significantly limit men's life quality. Among this field of research, photo-induced therapies, such as photodynamic and photothermal therapies (PDT and PTT), might represent an important advancement in PC treatment due to their extremely localized and controlled cytotoxic effect, as well as their low incidence of side effects and tumor resistance occurrence. Based on these considerations, this review aims to gather and discuss the last 5-years literature reports dealing with the synthesis and biological activity of molecular conjugates and nano-platforms for photo-induced therapies as co-adjuvant or combined therapeutic modalities for the treatment of localized PC.

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Diagnostic and therapeutic research on ultrasound microbubble/nanobubble contrast agents (Review)

Cited by 35 publications

References 54 publications

Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

Microbubble-Mediated Ultrasound Outweighs Low-Intensity Pulsed Ultrasound on Osteogenesis and Neovascularization in a Rabbit Model of Steroid-Associated Osteonecrosis

On-Demand and Long-Term Drug Delivery from Degradable Nanocapsules

Light-Induced Therapies for Prostate Cancer Treatment

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