Diagnostic and prognostic value of anti-CarP antibodies in a sample of Egyptian rheumatoid arthritis patients

Elsayed, Sahar A.; Esmail, Mohamed; Ali, Randa M; Mohafez, Omar Mahmoud Mohamed

doi:10.1007/s10067-019-04616-z

Cited by 9 publications

(2 citation statements)

References 35 publications

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“…Approximately 35 to 50% of RA subjects develop antibodies against carbamylated fetal bovine serum (FBS) (anti-CarP antibodies) (5,16,17), but the specific carbamylated antigens and their source remain unclear. We found that anti-CarP antibodies were significantly elevated in the two RA cohorts tested in this study, when compared to healthy volunteers (Fig.…”

Section: Ra Patients Develop Autoantibodies Against Cnet Proteinsmentioning

confidence: 99%

Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis

et al. 2020

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Formation of autoantibodies to carbamylated proteins (anti-CarP) is considered detrimental in the prognosis of erosive rheumatoid arthritis (RA). The source of carbamylated antigens and the mechanisms by which anti-CarP antibodies promote bone erosion in RA remain unknown. Here, we find that neutrophil extracellular traps (NETs) externalize carbamylated proteins and that RA subjects develop autoantibodies against carbamylated NET (cNET) antigens that, in turn, correlate with levels of anti-CarP. Transgenic mice expressing the human RA shared epitope (HLADRB1* 04:01) immunized with cNETs develop antibodies to citrullinated and carbamylated proteins. Furthermore, anti–carbamylated histone antibodies correlate with radiographic bone erosion in RA subjects. Moreover, anti–carbamylated histone–immunoglobulin G immune complexes promote osteoclast differentiation and potentiate osteoclast-mediated matrix resorption. These results demonstrate that carbamylated proteins present in NETs enhance pathogenic immune responses and bone destruction, which may explain the association between anti-CarP and erosive arthritis in RA.

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Section: Ra Patients Develop Autoantibodies Against Cnet Proteinsmentioning

confidence: 99%

Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis

et al. 2020

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“…Имеются данные об особенностях анти-Карб-позитивного субтипа РА. У анти-Карб-позитивных пациентов наблюдается более эрозивный артрит по данным рентгенографии, причем в большей степени это характерно для АЦЦП-негативных пациентов [3,9,11,12,[16][17][18]. Также с повышением анти-Карб ассоциировано интерстициальное заболевание легких как системное проявле-ние РА [19,20].…”

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Anti-carbamylated protein antibodies in ACCP-negative and ACCP-positive patients with rheumatoid arthritis

Dibrov,

Avdeeva,

Diatroptov

et al. 2024

Naučno-praktičeskaâ revmatologiâ

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Objective. Assess the level of antibodies to carbamylated proteins (anti-CarP) and analyse the clinical and immunological associations in patients with ACCP-negative and ACCP-positive variants of rheumatoid arthritis.Materials and methods. 150 patients with a reliable diagnosis of rheumatoid arthritis and 25 patients as healthy controls were included in the study. Depending on ACCP values, two groups of patients were recruited: ACCP-positive (n=75) and ACCP-negative (n=75). RA activity was assessed by the DAS28 (Disease Activity Score 28) index. Determination of antibodies to carbamylated proteins was performed by enzyme-linked immunosorbent assay (BlueGene Biotech, China). Quantitative determination of ACCP in serum was performed by enzyme immunoassay using a commercial reagent kit (AxisShield, UK; upper limit of normal 5.0 U/ml; Orgentec, Germany; upper limit of normal 20.0 U/ml).Results and discussion. Me for anti-CarP in patients with RA was 126.2 [100.83; 157.41] ng/ml and was statistically significantly higher (p<0.001) than healthy controls 88.89 [70.53; 107.75] ng/ml. Among all patients with RA, 50 (33.3%) were anti-Carp positive, 22 (29.3%) were anti-Carp(+) in the ACCP(+) group, 28 (37.3%) in the ACCP(–) group, and 1 (2%) volunteer from healthy controls (p=0.002). In ROC analysis to assess the diagnostic significance of anti-Carp for RA for all patients with RA, the area under the curve was 0.783±0.047 (95% CI: 0.691–0.874; p<0.001), with a cut-off point of 143 ng/ml, specificity 96%, sensitivity 36.7%.In the ACCP(+) RA group, the erosion count was statistically significantly higher (p=0.044) in anti-CarP(+) patients than in anti-CarP(–) patients. A weak direct correlation between anti-CarP and DAS28 was found in the ACCP(–) RA group.Conclusion. We studied the predictive value of anti-CarP as an adjuvant biomarker in ACCP(+) and ACCP(–) subtypes of RA. ACCP(+), anti-CarP(+) patients have a more “erosive” subtype of the disease than ACCP(+), anti-CarP(–) patients. In ACCP(+) patients, anti-CarP helps to identify a more erosive subtype of the disease, and among ACCP(–) patients, it helps to reduce the proportion of seronegative patients. Further studies are needed to determine the optimal standards for the laboratory diagnosis of anti-CarP and to clarify the diagnostic potential of these antibodies as part of the differential diagnosis of arthritis in other rheumatic diseases.

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Investigating the stabilisation of IFN-α2a by replica exchange molecular dynamics simulation

Chen²,

Dong³

et al. 2022

J Mol Model

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Diagnostic and prognostic value of anti-CarP antibodies in a sample of Egyptian rheumatoid arthritis patients

Cited by 9 publications

References 35 publications

Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis

Neutrophil-mediated carbamylation promotes articular damage in rheumatoid arthritis

Anti-carbamylated protein antibodies in ACCP-negative and ACCP-positive patients with rheumatoid arthritis

Investigating the stabilisation of IFN-α2a by replica exchange molecular dynamics simulation

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