Diagnostic and Prognostic Characteristics of Circulating Free DNA Methylation Detected by the Electrochemical Method in Malignant Tumors

Sun, Li-Yue; Du, Ziwei; Liu, Yuying; Li, Yanhong; Liu, Xiaomin; Wang, Ting; Shao, Jian Yong

doi:10.3390/cancers13040664

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…29 Another technique used is to measure the change in angle by attenuated total reflection, evaluating DNA methylation using fluorimetric, SERS, SPR, FRET, and colorimetric methods 30 between healthy DNA samples and those of diseased individuals. Electrochemical method 31 is another technique applied with the goal of diagnostics, studying differences between unmethylated and methylated DNA. Several studies confirm that changes occur in DNA at the structural level.…”

Section: ■ Introductionmentioning

confidence: 99%

DNA Hyperstructure

León-Paz-de-Rodríguez,

Rodríguez-León,

Iñiguez-Palomares

2024

ACS Omega

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This study presents a new procedure to condense DNA molecules and precipitate them onto a glass slide. The resulting DNA molecules undergo autonomous self-assembly, creating closed superstructures on the micrometer scale, which are called DNA hyperstructures. These structures can be observed using lowmagnification (4×) light microscopy. Precisely controlling the alcohol/glacial acetic acid ratio and DNA concentration during precipitation enabled the regulation of structure compaction on the slide. The alcohol/glacial acetic acid ratio is inversely proportional to the DNA concentration to achieve optimal compaction on the slide. Confocal microscopy fluorescence analysis of DNA extracts stained with DAPI shows that nucleic acids self-assemble to form structures during precipitation on the slide. This methodology is relevant since it facilitates the precipitation and visualization of DNA, regardless of its origin or molecular weight. To confirm its versatility, results with DNA extracted from human peripheral blood, the Lambda virus, and plasmid pBR322 are presented. The study examined the morphological features of DNA hyperstructures in both healthy individuals and those diagnosed with different medical conditions or illnesses, revealing distinct patterns specific to each case. This innovative technology has potential for disease detection in peripheral blood samples, ranging from cancer and Alzheimer's disease to determining the gender of the gestational product at an early stage.

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Section: ■ Introductionmentioning

confidence: 99%

DNA Hyperstructure

León-Paz-de-Rodríguez,

Rodríguez-León,

Iñiguez-Palomares

2024

ACS Omega

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ctDNA as a prognostic biomarker in resectable CLM: Systematic review and meta-analysis

Wang

Zhao

et al. 2023

Open Life Sciences

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Cell-free circulating tumor DNA (ctDNA) is synthesized by tumor cells, including metastatic tumors, and circulates in the bloodstream. Evidence suggests that ctDNA is a potential predictive and prognostic biomarker for colorectal cancer (CRC), but its predictive efficacy in detecting CRC liver metastasis (CLM) remains unclear. Additionally, its utility in the clinical setting needs further investigation. We conducted a meta-analysis to determine the utility of ctDNA as a biomarker for predicting the prognosis of CLM and investigate the relationship between CLM and ctDNA positivity. A literature search was performed in electronic databases to identify relevant studies published up to March 19, 2022. We retrieved data on overall survival (OS), disease-free survival (DFS), and recurrence-free survival (RFS) for both ctDNA-positive and ctDNA-negative colorectal liver metastasis (CLM) patients from the selected articles. Hazard ratios (HRs) were also calculated for these survival outcomes analysis was also performed. The stability of the combined meta-analysis was verified by sensitivity analysis and publication bias evaluation. Ten trials were included, and 615 patients were evaluated. In patients with CLM, pooled HRs revealed a substantial link between ctDNA positivity and RFS/DFS. Subgroup analysis revealed that ctDNA had a prospective detection value. Sensitivity analysis and publication bias evaluation indicated stable results. Although the results on pooled HR for OS suggested that ctDNA-positive patients had a shorter survival time, their pooled HRs had a relatively evident heterogeneity, and sensitivity analysis and publication bias evaluation indicated that pooled HRs were extremely unstable. In conclusion, our results demonstrate that ctDNA appears to be a prognostic biomarker for resectable CLM patients.

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Advances in detection of carcinoembryonic antigen for diagnosis of esophageal cancer based on electrochemical aptamer sensors

Wang¹

2022

International Journal of Electrochemical Science

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The determination of carcinoembryonic antigen (CEA) has been an hot topic in the medical analysis due to the positive correlation between its elevation and many cancers. How to detect CEA with high sensitivity and speed has therefore been of great interest in analytical chemistry. Electrochemical analytical techniques have been considered well suited for screening specific molecules due to their high sensitivity and rapidity. This review summarizes the advances in electrochemical analytical techniques for CEA detection. We divided the electrochemical sensor into impedance, amperage, voltammetry, potentiometry, electrogenerated chemiluminescence (ECL), and photoelectrochemical (PEC). We presented their primary methodologies and important works in them separately. In addition, we compared the performance of recently published sensors through a table. Finally, we present perspectives on this topic based on the review.

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Diagnostic and Prognostic Characteristics of Circulating Free DNA Methylation Detected by the Electrochemical Method in Malignant Tumors

Cited by 3 publications

References 41 publications

DNA Hyperstructure

DNA Hyperstructure

ctDNA as a prognostic biomarker in resectable CLM: Systematic review and meta-analysis

Advances in detection of carcinoembryonic antigen for diagnosis of esophageal cancer based on electrochemical aptamer sensors

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