Diagnostic and Epitope Mapping Potential of Single-Chain Antibody Fragments Against Foot-and-Mouth Disease Virus Serotypes A, SAT1, and SAT3

Chitray, Melanie; Opperman, Pamela Anne; Rotherham, Lia S.; Fehrsen, Jeanni; Wyngaardt, Wouter Van; Frischmuth, Janine; Rieder, Elizabeth; Maree, Francois Frederick

doi:10.3389/fvets.2020.00475

Cited by 6 publications

(3 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Of these, four (one each in VP2 and VP3 and two in VP1) had very high scores (above 0.9; Table 3 a). These residues could be of antigenic significance, as they are either part of neutralizing antigenic sites, for example VP1 47 (antigenic site 3), or located in an adjacent area that are known to strongly influence the binding of neutralizing mabs [ 35 , 36 , 37 ]. All these residues were found to be surface-exposed, except one in VP2 (VP2-163) and two residues in VP3 (VP3-36 and VP3-99) ( Figure 3 A–C).…”

Section: Resultsmentioning

confidence: 99%

“…Of the residues identified in VP1, VP1-141 was within antigenic site 1, while VP1-149 formed antigenic site 5 in serotype O viruses [ 35 , 42 ]. In addition, the antigenic significance of both VP1-149 and -150 have been reported in serotype A viruses [ 37 ]. Studying the antigenic and genetic characteristics of the serotype O/Cathay viruses between 2010 and 2013 could elucidate the antigenic drift of these viruses better, which was not possible within the scope of this study.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013–2018) from Southeast Asia

Upadhyaya

Mahapatra

Mioulet

et al. 2021

Viruses

View full text Add to dashboard Cite

Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals with serious economic consequences. FMD is endemic in Southeast Asia (SEA) and East Asia (EA) with the circulation of multiple serotypes, posing a threat to Australia and other FMD-free countries. Although vaccination is one of the most important control measures to prevent FMD outbreaks, the available vaccines may not be able to provide enough cross-protection against the FMD viruses (FMDVs) circulating in these countries due to the incursion of new lineages and sub-lineages as experienced in South Korea during 2010, a FMD-free country, when a new lineage of serotype O FMDV (Mya-98) spread to the country, resulting in devastating economic consequences. In this study, a total of 62 serotype O (2013–2018) viruses selected from SEA and EA countries were antigenically characterized by virus neutralization tests using three existing (O/HKN/6/83, O/IND/R2/75 and O/PanAsia-2) and one putative (O/MYA/2009) vaccine strains and full capsid sequencing. The Capsid sequence analysis revealed three topotypes, Cathay, SEA and Middle East-South Asia (ME-SA) of FMDVs circulating in the region. The vaccines used in this study showed a good match with the SEA and ME-SA viruses. However, none of the recently circulating Cathay topotype viruses were protected by any of the vaccine strains, including the existing Cathay topotype vaccine (O/HKN/6/83), indicating an antigenic drift and, also the urgency to monitor this topotype in the region and develop a new vaccine strain if necessary, although currently the presence of this topotype is mainly restricted to China, Hong Kong, Taiwan and Vietnam. Further, the capsid sequences of these viruses were analyzed that identified several capsid amino acid substitutions involving neutralizing antigenic sites 1, 2 and 5, which either individually or together could underpin the observed antigenic drift.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013–2018) from Southeast Asia

Upadhyaya

Mahapatra

Mioulet

et al. 2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Besides, the antigenic sites on VP3 were targeted by most of NAbs [11,12]. Unlike A5 and A10 strains, the G-H loop on VP1 seems a major antigenic site on A12, A22 and A24 strains [13][14][15], and the key determinations involving this site are diverse, consisting of both conformational and linear epitopes [16,17]. Obviously, the antigenic structure of divergent serotype A reported above was only focused on some strains of specific lineage, and the conserved antigenic structure and viral evolution of FMDV serotype A were less understood.…”

Section: Introductionmentioning

confidence: 99%

Conserved antigen structures and antibody-driven variations on foot-and-mouth disease virus serotype A revealed by bovine neutralizing monoclonal antibodies

Li,

He,

Wang

et al. 2023

PLoS Pathog

View full text Add to dashboard Cite

Foot-and-mouth disease virus (FMDV) serotype A is antigenically most variable within serotypes. The structures of conserved and variable antigenic sites were not well resolved. Here, a historical A/AF72 strain from A22 lineage and a latest A/GDMM/2013 strain from G2 genotype of Sea97 lineage were respectively used as bait antigen to screen single B cell antibodies from bovine sequentially vaccinated with A/WH/CHA/09 (G1 genotype of Sea97 lineage), A/GDMM/2013 and A/AF72 antigens. Total of 39 strain-specific and 5 broad neutralizing antibodies (bnAbs) were isolated and characterized. Two conserved antigenic sites were revealed by the Cryo-EM structures of FMDV serotype A with two bnAbs W2 and W125. The contact sites with both VH and VL of W125 were closely around icosahedral threefold axis and covered the B-C, E-F, and H-I loops on VP2 and the B-B knob and H-I loop on VP3; while contact sites with only VH of W2 concentrated on B-B knob, B-C and E-F loops on VP3 scattering around the three-fold axis of viral particle. Additional highly conserved epitopes also involved key residues of VP158, VP1147 and both VP272 / VP1147 as determined respectively by bnAb W153, W145 and W151-resistant mutants. Furthermore, the epitopes recognized by 20 strain-specific neutralization antibodies involved the key residues located on VP3 68 for A/AF72 (11/20) and VP3 175 position for A/GDMM/2013 (9/19), respectively, which revealed antigenic variation between different strains of serotype A. Analysis of antibody-driven variations on capsid of two virus strains showed a relatively stable VP2 and more variable VP3 and VP1. This study provided important information on conserve and variable antigen structures to design broad-spectrum molecular vaccine against FMDV serotype A.

show abstract

Detection of Harmful Microbes

Demchenko

2023

Introduction to Fluorescence Sensing

View full text Add to dashboard Cite

Diagnostic and Epitope Mapping Potential of Single-Chain Antibody Fragments Against Foot-and-Mouth Disease Virus Serotypes A, SAT1, and SAT3

Cited by 6 publications

References 72 publications

Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013–2018) from Southeast Asia

Molecular Basis of Antigenic Drift in Serotype O Foot-and-Mouth Disease Viruses (2013–2018) from Southeast Asia

Conserved antigen structures and antibody-driven variations on foot-and-mouth disease virus serotype A revealed by bovine neutralizing monoclonal antibodies

Detection of Harmful Microbes

Contact Info

Product

Resources

About