Diagnostic Amsel criteria compared standardized method of Gram stain for the diagnosis of bacterial vaginosis

Moussavi, Ziaeddine; Behrouzi, Roxana

doi:10.1016/j.ics.2004.05.094

Cited by 10 publications

(10 citation statements)

References 11 publications

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“…These findings were consistent with other studies (Table 5). 6,7 Among all Amsel criteria, PH>4.5 had highest sensitivity (97.50%), and lowest specificity (77.78%), which was similar to various other studies. [8][9][10][11] It can be explained by the fact that measurement of vaginal pH is easily affected by the presence of blood or semen or application of lubricant gel which can increase the vaginal ph.…”

Section: Resultssupporting

confidence: 89%

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Comparison of Amsel’s criteria and Nugent’s criteria for diagnosis of bacterial vaginosis in tertiary care centre

Bansal

Garg

2019

Int J Reprod Contracept Obstet Gynecol

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Background: Bacterial vaginosis is an imbalance in the ecology of the normal vaginal flora which is characterized by depletion of lactobacilli, and proliferation of anaerobic bacteria. It most often manifests clinically as a vaginal pH of > 4.5, presence of thin whitish homogenous vaginal discharge, detection of “clue” cells and presence of an amine odour after the addition of 10 percent potassium hydroxide. These anaerobic bacteria through specific products stimulate the decidual tissue causing an increase of cytokine level, release of phospholipase A2 and prostaglandins leading to preterm labor, premature rupture of membranes, chorioamnionitis and development of PID following abortion. To compare Amsel Criteria and Nugent Criteria for diagnosis of bacterial vaginosis.Methods: A cross sectional study involving 260 patients with preterm and term labour was conducted at a tertiary care hospital in North India. BV was determined to be present or absent on the basis of Amsel’s criteria and Nugent’s criteria. Pearson’s chi-square test was used to demonstrate the difference between both groups with respect to various categorical data.Results: Amsel’s criteria and Nugent’s criteria were reliable diagnostic methods. As compared to Nugent scoring system, Amsel’s criteria had sensitivity of 75%, specificity of 95%, positive predictive value of 90% and negative predictive value of 86%.Conclusions: Although the Amsel’s criteria is a convenient and inexpensive method of diagnosing bacterial vaginosis, it is not always reliable. Nugent’s criteria is considered as a gold standard for the diagnosis of bacterial vaginosis but it requires an experienced slide reader and considerable time and skill. If lab equipment is not available as in many developing countries, the diagnosis of BV can be simplified by using a combination of any two Amsel’s criteria like vaginal pH and whiff test which had highest sensitivity and specificity (90.19% and 97.78%) respectively as seen in present study.

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Section: Resultssupporting

confidence: 89%

“…In present study whiff test had best sensitivity (92.50%) and specificity (90.00%) which was similar to other studies. 7 The combination of PH and whiff test had high sensitivity (90.19%) and specificity (97.78%) so recommended for evaluation of vaginosis similar to previous studies. [13][14]…”

Section: Resultssupporting

confidence: 66%

Comparison of Amsel’s criteria and Nugent’s criteria for diagnosis of bacterial vaginosis in tertiary care centre

Bansal

Garg

2019

Int J Reprod Contracept Obstet Gynecol

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“…Landers et al (28) estudiaron una población de 598 mujeres (con una prevalencia de 62 % de VB diagnosticada por coloración de Gram) y encontraron un mayor rendimiento para el diagnóstico clínico de VB, con una sensibilidad de 92 %, una especificidad de 77 % y valor predictivo positivo de 80 %. Moussavi y Behrouzi (29), en una población de 102 pacientes, encontraron que la presencia de flujo vaginal presenta una sensibilidad de 63 % y una especificidad de 80 % para el diagnóstico de VB, siendo la especificidad mayor a la encontrada en el presente estudio. La prueba de aminas o de whiff, como único criterio diagnóstico, tuvo una una sensibilidad del 99,3 % y una especificidad del 99,8 % en el presente estudio.…”

Section: Discussionunclassified

“…En un estudio de Thulkar et al (30), en un grupo de mujeres con flujo vaginal anormal, y con una prevalencia de VB de 39 %, la prueba de aminas tuvo una sensibilidad de 94,0 %, similar a la de este estudio, pero con una menor especificidad, de 67,7 %. Para la prueba de aminas, otras investigaciones, como la de Moussavi y Behrouzi (29), encontraron características operativas inferiores a la de nuestro estudio (sensibilidad de 75 %, especificidad de 73 %).…”

Section: Discussionunclassified

Características operativas del diagnóstico clínico con y sin pruebas de consultorio (pH y prueba de aminas) para el diagnóstico de vaginosis bacteriana, en pacientes sintomáticas en Bogotá, Colombia

Mejía-Pérez¹,

Angel‐Müller²,

Rodríguez-Hernández³

et al. 2015

Rev. Colomb. Obstet. Ginecol.

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Objetivo: evaluar las características operativas del diagnóstico clínico (síntomas y signos) solo y con pruebas de diagnóstico en el consultorio (pH y prueba de aminas), teniendo como patrón de oro el puntaje de Nugent, para el diagnóstico de vaginosis bacteriana.Materiales y métodos: estudio de validez diagnóstica, ensamblado en un estudio de corte transversal en pacientes con síntomas de infección del tracto genital inferior, quienes consultaron a tres centros de Bogotá en 2010. Se calculó un tamaño de muestra de 1400 participantes. Se realizó un muestreo secuencial. Se tomaron muestras de la pared vaginal para medición de pH, prueba de aminas y tinción de Gram para puntaje de para puntaje de Nugent, considerado como patrón de oro de la entidad. Las bacteriólogas estaban enmascaradas con respecto al diagnóstico clínico y a las pruebas de consultorio. Se midieron la sensibilidad y especificidad, razón de probabilidades RP (+) y RP (-). Se presentan como proporciones con su respectivo intervalo de confianza del 95%.Resultados: la prevalencia de VB fue de 39.6 %, las características operativas de las pruebas con sus respectivos IC 95 %, con el puntaje de Nugent como patrón de oro, fueron: diagnóstico clínico, sensibilidad 75 % (71-78), especificidad 54 % (51-57), pH ≥ 5 sensibilidad 99,3 % (98,2-99.8), especificidad 0,57 % (0,2-1,3); prueba de aminas, sensibilidad 99,3 % (98,2- 99,8 %), especificidad 99,8 % (99,2-100 %); diagnóstico clínico, más prueba de aminas, más pH ≥ 5,0, sensibilidad 74 % (71-78), especificidad 100 % (99-100).Conclusiones: el diagnóstico clínico es una estrategia efectiva para el diagnóstico de VB; la especificidad mejora cuando se combina con la prueba de aminas y el pH vaginal. La prueba de aminas presentó excelente sensibilidad y especificidad, y es útil para mejorar el diagnóstico de VB.

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“…The three dominant bacteria were stained according to the Gram procedure (Moussavi and Behrouzi 2004). The results showed that the bacterium of Colony 1 was rod-shaped with uniform red color observed by the microscope, indicating it was gram negative bacterium.…”

Section: Identification Of Dominant Bacteriamentioning

confidence: 99%

Screening Predominant Bacteria and Construction of Efficient Microflora for Treatment of Papermaking White Water

Lan¹,

Zhang²,

Yang³

et al. 2018

BioResources

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Three strains of bacteria were isolated and purified from activated sludge for white water treatment in the laboratory. These strains were identified as Bacillus subtilis, Bacillus cereus, and Virgibacillus pantothenticus through a morphological analysis, the MIDI Sherlock automatic microbial identification system, and 16S rRNA methods. The results of the construction of efficient microflora for white water showed that a mass percentage ratio of B. subtilis, B. cereus, and V. pantothenticus of 50%:35%:15% achieved an optimal treatment effect. Analysis by gas chromatograph-mass spectrometer (GC-MS) established that the content of characteristic pollutants in white water decreased notably after treatment with the efficient microflora, and detected the intermediate products of short chain fatty acids, alcohols, and other compounds. Moreover, through measuring the removal rate of chemical oxygen demand (COD), electrical conductivity, and cationic demand (CD), the optimal retention time for white water treatment with the efficient microflora was 4 h to 6 h, and when the removal rate of COD reached approximately 90%, the electrical conductivity and the cationic demand were reduced to lower values.

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Diagnostic Amsel criteria compared standardized method of Gram stain for the diagnosis of bacterial vaginosis

Cited by 10 publications

References 11 publications

Comparison of Amsel’s criteria and Nugent’s criteria for diagnosis of bacterial vaginosis in tertiary care centre

Comparison of Amsel’s criteria and Nugent’s criteria for diagnosis of bacterial vaginosis in tertiary care centre

Características operativas del diagnóstico clínico con y sin pruebas de consultorio (pH y prueba de aminas) para el diagnóstico de vaginosis bacteriana, en pacientes sintomáticas en Bogotá, Colombia

Screening Predominant Bacteria and Construction of Efficient Microflora for Treatment of Papermaking White Water

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