Diagnostic accuracy of enzyme-linked immunosorbent assays (ELISA) to detect anti-skin autoantibodies in autoimmune blistering skin diseases: A systematic review and meta-analysis

Atypical presentations of bullous pemphigoid: Clinical and immunopathological aspects

“…Circulating autoantibodies can be detected by enzyme-linked immunosorbent assay (ELISA), with sensitivity up to 100% when various ELISAs using the NC16A domain and other extracellular portions of BP180 or of BP230 are used together [1,77,140,141]. Nowadays, ELISA has mostly replaced immunoblot and immunoprecipitation techniques, which are valid techniques but not very easy for routine testing [137].…”

Autoimmune blistering diseases in females: a review

“…This is because it is believed that there may be a small risk of prilocaine fetal methemoglobinemia and uterine blood flow disruption that could happen secondary to local anesthetics use during the skin biopsy (Hrgovic, 1990, Nau, 1985, Voorbrood et al, 1982). Meanwhile, newer and safer diagnostic methods, including enzyme-linked immunosorbent assay (ELISA) of BP180 and desmoglein 1 and 3 autoantibodies, indirect immunofluorescence, and biochip immunofluorescence microscopy, should be considered as potential alternatives to biopsying (Russo et al, 2014, Sardy et al, 2013, Tampoia et al, 2012). …”

Autoantibody Levels and Clinical Disease Severity in Patients with Pemphigus: Comparison of Aggregated Anti-desmoglein ELISA Values and Indirect Immunofluorescence Titres

“…In the late 1990s, enzyme-linked immunosorbent assays (ELISA) were introduced, using recombinant human Dsg (Dsg1 and Dsg3). These test systems demonstrated higher sensitivity and specificity than IIF for the detection of IgG serum autoantibodies (9,10). Yet, IIF still has its role in detecting autoantibodies which are outside of the epitopes used in the Dsg ELISA systems or directed against non-Dsg targets such as desmocollins (11).…”