Diagnostic Accuracy of Dried Blood Spots Collected on HemaSpot HF Devices Compared to Venous Blood Specimens To Estimate Measles and Rubella Seroprevalence

Prosperi, Christine; Kaduskar, Ojas; Bhatt, Vaishali; Hasan, Alvira Z.; Thangaraj, Jeromie Wesley Vivian; Kumar, Muthusamy Santhosh; Sabarinathan, R.; Kumar, Saravana; Duraiswamy, Augustine; Deshpande, Gururaj; Thankappan, Ullas Padinjaremattathil; Chauhan, Sanjay; Kulkarni, Ragini; Bansal, Avi Kumar; Chaaithanya, Itta Krishna; Salvi, Neha; Sharma, Sandeep; Moss, William J.; Sangal, Lucky; Gupta, Nivedita; Murherkar, Manoj V.; Mehendale, Sanjay; Sapkal, Gajanan; Hayford, Kyla

doi:10.1128/msphere.01330-20

Cited by 5 publications

(3 citation statements)

References 21 publications

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“…First, voluntary participation entails a selection bias; possibly, participants who were vaccinated or who assumed an undiagnosed previous infection were more likely to participate in this serological survey. Secondly, we used dried blood spots for serology because of the less invasive finger-prick sampling [23,[51][52][53]. The quality of such results can be hampered depending on blood volume [23,53,54], but to avoid that, we only included samples with sufficient volume and confirmed results.…”

Section: Discussionmentioning

confidence: 99%

“…Secondly, we used dried blood spots for serology because of the less invasive finger-prick sampling [23,[51][52][53]. The quality of such results can be hampered depending on blood volume [23,53,54], but to avoid that, we only included samples with sufficient volume and confirmed results. Another limitation is that antibodies can decrease over time to an undetectable level [25,38,55,56], and among vaccinated participants aged 12 years and older, previous infections could be masked by a comparatively faster decrease in anti-N antibodies than anti-S antibodies [24].…”

Section: Discussionmentioning

confidence: 99%

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SARS-CoV-2 Seroprevalence in a Berlin Kindergarten Environment: A Cross-Sectional Study, September 2021

Bernhard,

Theuring,

van Loon

et al. 2024

Children

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SARS-CoV-2 serology may be helpful to retrospectively understand infection dynamics in specific settings including kindergartens. We assessed SARS-CoV-2 seroprevalence in individuals connected to kindergartens in Berlin, Germany in September 2021. Children, staff, and household members from 12 randomly selected kindergartens were interviewed on COVID-19 history and sociodemographic parameters. Blood samples were collected on filter paper. SARS-CoV-2 anti-S and anti-N antibodies were assessed using Roche Elecsys. We assessed seroprevalence and the proportion of so far unrecognized SARS-CoV-2 infections. We included 277 participants, comprising 48 (17.3%) kindergarten children, 37 (13.4%) staff, and 192 (69.3%) household members. SARS-CoV-2 antibodies were present in 65.0%, and 52.7% of all participants were vaccinated. Evidence of previous infection was observed in 16.7% of kindergarten children, 16.2% of staff, and 10.4% of household members. Undiagnosed infections were observed in 12.5%, 5.4%, and 3.6%, respectively. Preceding infections were associated with facemask neglect. In conclusion, two-thirds of our cohort were SARS-CoV-2 seroreactive in September 2021, largely as a result of vaccination in adults. Kindergarten children showed the highest proportion of non-vaccine-induced seropositivity and an increased proportion of previously unrecognized SARS-CoV-2 infection. Silent infections in pre-school children need to be considered when interpreting SARS-CoV-2 infections in the kindergarten context.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

SARS-CoV-2 Seroprevalence in a Berlin Kindergarten Environment: A Cross-Sectional Study, September 2021

Bernhard,

Theuring,

van Loon

et al. 2024

Children

View full text Add to dashboard Cite

show abstract

“…Dried blood analytes collected with HemaSpot have been applied as an alternative to DBS for serologic tests for several human viral pathogens [ 53 , 54 , 55 , 56 ]. While HemaSpot appears to be a promising technology, data on the usage of HemaSpot specimens for HIV molecular assays are scarce.…”

Section: Varied Analytes For Hivdrtmentioning

confidence: 99%

Overview of the Analytes Applied in Genotypic HIV Drug Resistance Testing

Sandstrom

2022

Pathogens

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The close monitoring of HIV drug resistance using genotypic HIV drug resistance testing (HIVDRT) has become essential for effective HIV/AIDS management at both individual and population levels. Over the years, a broad spectrum of analytes or specimens have been applied or attempted in HIVDRT; however, the suitability and performance of these analytes in HIVDRT and the clinical relevance of the results from them may vary significantly. This article provides a focused overview of the performance, strengths, and weaknesses of various analytes while used in HIVDRT, which may inform the optimal analytes selection in different application contexts.

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Measles Vaccines

Moss¹,

Strebel²

2023

Plotkin's Vaccines

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Diagnostic Accuracy of Dried Blood Spots Collected on HemaSpot HF Devices Compared to Venous Blood Specimens To Estimate Measles and Rubella Seroprevalence

Cited by 5 publications

References 21 publications

SARS-CoV-2 Seroprevalence in a Berlin Kindergarten Environment: A Cross-Sectional Study, September 2021

SARS-CoV-2 Seroprevalence in a Berlin Kindergarten Environment: A Cross-Sectional Study, September 2021

Overview of the Analytes Applied in Genotypic HIV Drug Resistance Testing

Measles Vaccines

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