Diagnostic accuracy of DNA methylation for head and neck cancer varies by sample type and number of markers tested

Xu, Jianrong; Chen, Guan; Jiang, Xuejun; Li, Hong

doi:10.18632/oncotarget.12219

Cited by 12 publications

(9 citation statements)

References 54 publications

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“…The threshold effect is one of the leading causes of heterogeneity in a diagnostic meta-analysis [ 38 ], and mainly caused by the different cut-off values used in individual studies [ 39 ]. Heterogeneity generated by non-threshold effect is usually addressed via pooled DOR [ 30 ].…”

Section: Discussionmentioning

confidence: 99%

The roles of ncRNAs in the diagnosis, prognosis and clinicopathological features of breast cancer: a systematic review and meta-analysis

et al. 2017

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BackgroundA number of studies have shown that noncoding RNAs (ncRNAs) are abnormally expressed in breast cancers. However, the roles of ncRNAs remain unclear in breast cancer. Here, we aim to investigate the potential diagnostic and prognostic roles of ncRNAs in breast cancer.MethodsComprehensive literature search in Medline and Web of Science and a meta-analysis were performed to identify the association between ncRNAs and diagnosis, prognosis, and clinicopathological features of breast cancer.ResultsA total of 103 eligible studies, involving16, 828 independent participants, were included in the meta-analysis. In total, there were 98 individual and 11 grouped ncRNAs. 51 studies were eligible for survival analysis, 27 studies were eligible for diagnostic analysis, and 46 studies were eligible for clinicopathological features analysis. The abnormal expression of ncRNAs is associated with OS, RFS and PFS in breast cancer patients. For the diagnosis value of ncRNAs, the pooled OR and 95% CI for sensitivity, specificity, DOR and AUC on all ncRNAs were 0.83 [95% CI: 0.82- 0.84], 0.80 [95% CI: 0.79- 0.82], 24.77 [95% CI: 17.44- 35.16] and 0.9037, respectively. The analysis showed that downregulation of ncRNAs in breast cancer was associated with decreased risk of LNM, increased tumor size and PR expression, whereas, upregulation of ncRNAs was associated with increased HER2 expression.ConclusionsHigh expression of ncRNAs was associated with poor OS, RFS, and PFS, while low expression of ncRNAs was related to favorable OS and RFS. Meanwhile, ncRNAs have potential diagnostic value for breast cancer.

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Section: Discussionmentioning

confidence: 99%

The roles of ncRNAs in the diagnosis, prognosis and clinicopathological features of breast cancer: a systematic review and meta-analysis

et al. 2017

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“…The available evidence suggests that diagnostic accuracy of DNA methylation for HNSCCs varies according the sample type and number of markers tested (7,25).…”

Section: Discussionmentioning

confidence: 99%

Analysis of salivary detection of P16INK4A and RASSF1A promoter gene methylation and its association with oral squamous cell carcinoma in a Colombian population

González-Pérez¹,

Isaza-Guzmán²,

Arango-Pérez³

et al. 2020

J Clin Exp Dent

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Background: Epigenetic factors play a fundamental role in the etiopathogenesis of oral squamous cell carcinoma (OSCC). This study evaluated if salivary detection of P16INK4A/RASSF1A gene promoter methylation might be linked to the clinical/histological features of OSCC in a Colombian population. Material and Methods: Methylation-specific polymerase chain reaction (MSP-PCR) was used to detect the methylation frequency of P16INK4A/RASSF1A genes in DNA obtained from whole saliva collected of 40 healthy controls (HC) and 43 OSCC patients. Determination of the clinical performance of MSP-PCR assay was based on standard algorithms derived from two-way contingency table analysis. The association of methylation status of targeted genes with OSCC was analyzed in a multivariate binary logistic regression model. Results: There were significantly higher proportions of promoter methylation of these target genes in OSCC patients when compared with HC. The analysis of single methylated genes showed high specificity, good positive and negative predictive values, but was accompanied by a low sensitivity. OSCC cases with clinical stage III/IV, poorly differentiated, and severe cellular atypia showed a significantly greater proportion of methylated than that of unmethylated targeted genes in saliva samples. Logistic regression analysis indicated an independent association of P16INK4A and RASSF1A promoter methylation with OSCC diagnosis. A significant interaction effect between ageing and P16INK4A promoter methylation was also detected.

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“…On the other hand, this meta-analysis showed that methylation of RASSF1A gene in HBV-positive carcinoma tissues was significantly higher than HBV-negative carcinoma tissues, while the same phenomenon was not found in carcinoma serums. The effects of this gene methylation in two sample types are different, due to the diversities of specificity and sensitivity in different sample types [25]. Also, subgroup meta-analysis by geographical populations found that methylation of GSTP1 was significant in both China and Japan.…”

Section: Discussionmentioning

confidence: 99%

Association between gene methylation and HBV infection in hepatocellular carcinoma: A meta-analysis

Zhang¹,

Huang²,

Sui³

et al. 2019

J. Cancer

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Gene methylation is an epigenetic alteration in hepatocellular carcinoma (HCC), and hepatitis B virus (HBV) plays a crucial role in carcinogenesis of HCC. However, the association between gene methylation and HBV infection in HCC remains unclear. In our study, we conducted a comprehensive meta-analysis to evaluate the association. A total of 1,148 studies were initially retrieved from some literature database. After a four-step filtration, we obtained 69 case-control studies in this meta-analysis. Our results showed six genes (p16, RASSF1A, GSTP1, APC, p15 and SFRP1) in HBV-positive carcinoma tissues, one gene (GSTP1) in HBV-positive adjacent tissues and two gene (p16 and APC) in HBV-positive carcinoma serums, which were significantly hypermethylated. Subgroup meta-analysis by geographical populations revealed that GSTP1 methylation was significantly higher in HBV-positive carcinoma tissues in China and Japan. In addition, p16 and RASSF1A methylation was significantly higher in China but not in Japan. Our study indicated that HBV infection could induce DNA methylation in HCC and DNA methylation could lead to the development of HBV-related HCC.

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Diagnostic accuracy of DNA methylation for head and neck cancer varies by sample type and number of markers tested

Cited by 12 publications

References 54 publications

The roles of ncRNAs in the diagnosis, prognosis and clinicopathological features of breast cancer: a systematic review and meta-analysis

The roles of ncRNAs in the diagnosis, prognosis and clinicopathological features of breast cancer: a systematic review and meta-analysis

Analysis of salivary detection of P16INK4A and RASSF1A promoter gene methylation and its association with oral squamous cell carcinoma in a Colombian population

Association between gene methylation and HBV infection in hepatocellular carcinoma: A meta-analysis

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