Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients

Bravo, Dayana; Blanquer, José; Tormo, Mar; Aguilar, Gerardo; Borrás, Rafael; Solano, Carlos; Clari, María Ángeles; Costa, Elisa; Muñoz-Cobo, Beatriz; Argüeso, Mónica; Pineda, José Roberto; Navarro, David

doi:10.1016/j.ijid.2011.01.003

Cited by 58 publications

(24 citation statements)

References 21 publications

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“…In our research in DNA-based detection systems, we found genetic material from P aeruginosa, A baumannii, and A fumigatus in patients with blood cultures that showed no growth. This finding does not seem to depend on the characteristics of the patients, as it has also been reported in other studies focused on immunosuppressed patients, 34,35 transplant recipients, 36 and patients with sepsis. 11,37 SeptiFast has some disadvantages, including false-negatives and problems derived from the technique itself.…”

Section: Discussionsupporting

confidence: 86%

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Suberviola

Márquez-López

Castellanos-Ortega

et al. 2015

American Journal of Critical Care

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Objective To compare the utility of a multiplex polymerase chain reaction system (SeptiFast) and blood cultures for detecting bacteria and fungi in blood samples from patients with severe sepsis or septic shock. Methods In a prospective observational study, whole blood samples for SeptiFast testing and for culture were collected on admission from all patients with severe sepsis or septic shock admitted to the intensive care unit between July 2011 and September 2012. SeptiFast results were compared with blood and other culture results. Results The probability of at least 1 microorganism being isolated at 6 hours was 13-fold higher with the SeptiFast test than with blood cultures (relative risk, 13.5; 95% CI, 5.05-36.06). Unlike culture results, SeptiFast test results were not associated with previous antibiotic consumption. The median time to the first positive blood culture result was 17 hours; SeptiFast results were available in 6 hours. SeptiFast detected genetic material from potentially multiresistant microorganisms in patients whose blood cultures showed no growth at all. Conclusions The SeptiFast test provided quicker microbiological diagnosis and identified significantly more microorganisms than blood cultures did, particularly when samples were collected after antibiotic therapy had started or infections were due to resistant bacteria and yeast.

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Section: Discussionsupporting

confidence: 86%

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Suberviola

Márquez-López

Castellanos-Ortega

et al. 2015

American Journal of Critical Care

View full text Add to dashboard Cite

show abstract

“…These results stand in contrast to the results of our study, where the blood cultures showed a higher pathogen detection rate. Other recent research is in line with our results: studies investigating patients with suspected sepsis in the emergency department [21], patients with malignancies and sepsis [22], critically ill neutropenia patients [23] and etiological diag- nosis of community-onset bloodstream infections [24]. The other two PCR assays (SepsiTest® and VYOO®) are less well studied in respect to sepsis.…”

Section: Discussionsupporting

confidence: 88%

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Schreiber

Nierhaus

Braune

et al. 2013

Med Klin Intensivmed Notfmed

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“…Multiplex realtime PCR assays, on the other hand, facilitate the rapid identification of pathogens and are a promising approach for routine use. Several studies have shown the value of this diagnostic tool for rapid detection of bloodstream pathogens, particularly in pretreated patients (4,5,25). Also, for clinical specimens other than blood, these assays show a great potential for diagnosing bacterial infections (21).…”

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confidence: 99%

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

et al. 2012

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A new commercially available universal 16S and 18S rRNA gene PCR test, which is followed by sequence analysis of amplicons (SepsiTest), was evaluated for rapid identification of pathogens in the diagnosis of bone and joint infections. Eighty-three orthopedic samples and 21 specimens from other normally sterile body sites collected from 84 patients were analyzed in parallel by culture and PCR for detection of bacteria and fungi. Compared to culture, the diagnostic sensitivity and specificity of PCR were 88.5% and 83.5%, respectively. The detection rate of PCR (34.6%) was higher than that of bacterial culture (25.0%) as a consequence of the presence of fastidious and noncultivable species in samples and antibiotic treatment of patients. Thirteen culturenegative infections were identified by PCR, and PCR was able to detect culture-proven polymicrobial infections. On the other hand, three samples were culture positive but PCR negative. SepsiTest was demonstrated to be a valuable supplemental tool in the rapid detection of bacteria, especially for fastidious and noncultivable organisms, allowing earlier initiation of pathogenadapted therapy in patients with bone and joint infections.

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Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients

Abstract: The SeptiFast assay is a valuable ancillary method for the diagnosis of bloodstream infections in neutropenic and ICU patients. In these clinical settings, results of the SeptiFast assay may lead to a more targeted antibiotic therapy early after the onset of fever.

Cited by 58 publications

References 21 publications

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Microbiological Diagnosis of Sepsis: Polymerase Chain Reaction System Versus Blood Cultures

Comparison of three different commercial PCR assays for the detection of pathogens in critically ill sepsis patients

Improvement of Detection of Bacterial Pathogens in Normally Sterile Body Sites with a Focus on Orthopedic Samples by Use of a Commercial 16S rRNA Broad-Range PCR and Sequence Analysis

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