Diagnostic accuracy and distress associated with oropharyngeal suction in cystic fibrosis

Doumit, Michael; Belessis, Yvonne; Stelzer-Braid, Sacha; Mallitt, Kylie‐Ann; Rawlinson, William D.; Jaffé, Adam

doi:10.1016/j.jcf.2015.09.001

Cited by 14 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Participants in the younger cohort had annual bronchoalveolar lavage (BAL) as part of an annual surveillance programme separate to this study, and some participants in the older cohort also had additional BAL in 10 instances for clinical reasons (respiratory or symptomatic deterioration). Participants undergoing BAL were asked to provide a paired flocked nasal swab sample, OPS and BAL sample . In a subset of these participants, a DAR™ barrier filter (Covidien) (used to filter exhaled air from the ventilatory circuit during each bronchoscopy) was collected following each procedure for viral analysis.…”

Section: Methodsmentioning

confidence: 99%

“…Participants undergoing BAL were asked to provide a paired flocked nasal swab sample, OPS and BAL sample. 11 In a subset of these participants, a DAR™ barrier filter (Covidien) (used to filter exhaled air from the ventilatory circuit during each bronchoscopy) was collected following each procedure for viral analysis.…”

Section: Participants and Participant Samplesmentioning

confidence: 99%

See 1 more Smart Citation

Association of rhinovirus with exacerbations in young children affected by cystic fibrosis: Preliminary data

Stelzer-Braid

Liu

Doumit

et al. 2017

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

Rhinovirus (RV) is a common respiratory viral infection linked to worsening of chronic respiratory diseases including cystic fibrosis (CF) and asthma. RV was tested by RT-PCR in samples (n = 465) collected from the upper (nasal swab, oropharyngeal suction, and sputum) and lower (bronchoalveolar washings) respiratory tract of 110 children with CF. Air samples (n = 52) collected from the operating theatres and outpatient clinics were tested for RV. RV was found in 43% of children <5 years suffering an exacerbation, and 12% of older children (5-17 years). RV particles were detected in the air of clinic rooms. Detection of RV is important in better understanding viral infections in patients with CF.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Participants and Participant Samplesmentioning

confidence: 99%

Association of rhinovirus with exacerbations in young children affected by cystic fibrosis: Preliminary data

Stelzer-Braid

Liu

Doumit

et al. 2017

Journal of Medical Virology

Self Cite

View full text Add to dashboard Cite

show abstract

“…In theory, direct lung sampling in infants with CF by bronchoalveolar lavage (BAL) [8] would be most representative of the local presence and relative abundance of respiratory pathogens within LRT microbial communities. However, 1) because of the invasive nature of BAL, 2) because infants and young children with CF do not spontaneously expectorate sputum and 3) because of the lack of practical experience with obtaining induced sputum in these patients [8,9], clinicians generally use alternative specimens including nasal, nasopharyngeal (NP) and oropharyngeal (OP) samples for microbial surveillance [10][11][12][13][14][15]. Recent cross-sectional studies [16,17] in older children with CF have shown that the upper and lower airways on average harbour similar bacterial communities.…”

Section: Introductionmentioning

confidence: 99%

Concordance between upper and lower airway microbiota in infants with cystic fibrosis

et al. 2017

View full text Add to dashboard Cite

Nasopharyngeal and oropharyngeal samples are commonly used to direct therapy for lower respiratory tract infections in non-expectorating infants with cystic fibrosis (CF).We aimed to investigate the concordance between the bacterial community compositions of 25 sets of nasopharyngeal, oropharyngeal and bronchoalveolar lavage (BAL) samples from 17 infants with CF aged ∼5 months (n=13) and ∼12 months (n=12) using conventional culturing and 16S-rRNA sequencing.Clustering analyses demonstrated that BAL microbiota profiles were in general characterised by a mixture of oral and nasopharyngeal bacteria, including commensals like ,, and spp. and potential pathogens like , and spp. Within each individual, however, the degree of concordance differed between microbiota of both upper respiratory tract niches and the corresponding BAL.The inconsistent intra-individual concordance between microbiota of the upper and lower respiratory niches suggests that the lungs of infants with CF may have their own microbiome that seems seeded by, but is not identical to, the upper respiratory tract microbiome.

show abstract

“…Culture-based detection of P. aeruginosa in respiratory samples is the current gold standard for infection confirmation [27]. In children acquisition of sputum is not always possible and induced sputum, deep throat swabs or aspirates are used as a surrogate [10–12, 14]. Non invasive methods that could complement isolation of P. aeruginosa for earlier detection of infection in children with CF would be beneficial.…”

Section: Discussionmentioning

confidence: 99%

Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis

et al. 2016

View full text Add to dashboard Cite

BackgroundThe aim of this study was to assess whether serology and spirometry and the combination of both can complement culture-based detection for earlier recognition of Pseudomonas aeruginosa infection in children with cystic fibrosis.MethodsA 4 year longitudinal prospective study that included 67 Slovenian children with cystic fibrosis with a mean age of 10.5 years was conducted. Serology, spirometry and a scoring system combining serology and spirometry were assessed and compared. Infection was confirmed with isolation of Pseudomonas aeruginosa from respiratory samples.ResultsThere was a significantly positive correlation between serology and the combination of serology and spirometry and Pseudomonas aeruginosa isolation (P < 0.01 for both) and a significantly negative correlation between spirometry and Pseudomonas aeruginosa isolation (P < 0.05). An increase in serology for 1 ELISA unit increased the possibility of Pseudomonas aeruginosa isolation 1.6 times. A fall in FEV1% predicted for 10% increased the possibility of Pseudomonas aeruginosa isolation 9.8 times. Binary logistic regression analysis was used to determine the odds ratios and 95% confidence intervals for all three approaches. Serology had the highest specificity (0.80) and the combination of serology and spirometry the highest sensitivity (0.90). Both had a high negative predictive value (0.93 and 0.79 respectively).ConclusionUsing serology and the combination of serology and lung function measurement can be beneficial for earlier detection of infection with Pseudomonas aeruginosa in children with cystic fibrosis when done simultaneously with standard culture-based detection from respiratory samples.

show abstract

Diagnostic accuracy and distress associated with oropharyngeal suction in cystic fibrosis

Cited by 14 publications

References 27 publications

Association of rhinovirus with exacerbations in young children affected by cystic fibrosis: Preliminary data

Association of rhinovirus with exacerbations in young children affected by cystic fibrosis: Preliminary data

Concordance between upper and lower airway microbiota in infants with cystic fibrosis

Assessment of serology and spirometry and the combination of both to complement microbiological isolation for earlier detection of Pseudomonas aeruginosa infection in children with cystic fibrosis

Contact Info

Product

Resources

About