Diagnostic Ability of Methods Depicting Distress of Tumor-Bearing Mice

Xie, Wenjie; Kordt, Marcel; Palme, Rupert; Grambow, Eberhard; Vollmar, Brigitte; Zechner, Dietmar

doi:10.3390/ani11082155

Cited by 3 publications

(4 citation statements)

References 37 publications

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“…Subcutaneous (SC) tumor Xenograft models are widely used in cancer research, and nude mice are the most commonly used rodents for the establishment of xenografts models owing to their compromised immune system. , In the present study, a lung tumor xenograft model was successfully established and used to evaluate in vivo antitumor activity of the formulated CLA-coated PTX-SPIONs@HRH. Accordingly, a rapid tumor growth was recorded in the PBS (placebo) group, with tumors reaching an average volume of 1484.7 mm 3 at day 20 (Figure a).…”

Section: Resultsmentioning

confidence: 99%

Surface Immobilization of Anti-VEGF Peptide on SPIONs for Antiangiogenic and Targeted Delivery of Paclitaxel in Non-Small-Cell Lung Carcinoma

Ngema

Adeyemi

Marimuthu

et al. 2023

ACS Appl. Bio Mater.

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A design has been established for the surface decoration of superparamagnetic iron oxide nanoparticles (SPIONs) with anti-vascular endothelial growth factor peptide, HRH, to formulate a targeted paclitaxel (PTX) delivery nanosystem with notable tumor targetability and antiangiogenic activity. The design methodology included (i) tandem surface functionalization via coupling reactions, (ii) pertinent physicochemical characterization, (iii) in vitro assessment of drug release, antiproliferative activity, and quantification of vascular endothelial growth factor A (VEGF-A) levels, and (iv) in vivo testing using a lung tumor xenograft mouse model. Formulated CLA-coated PTX-SPIONs@HRH depicted a size and surface charge of 108.5 ± 3.5 nm and −30.4 ± 2.3 mV, respectively, and a quasi-spherical shape relative to pristine SPIONs. Fourier transform infrared (FTIR) analysis and estimation of free carboxylic groups supported the preparation of the CLA-coated PTX-SPIONs@HRH. CLA-coated PTX-SPIONs@HRH exhibited high PTX loading efficiency (98.5%) and sustained release in vitro, with a marked dose dependent anti-proliferative activity in A549 lung adenocarcinoma cells, complimented by an enhanced cellular uptake. CLA-coated PTX-SPIONs@HRH significantly reduced secretion levels of VEGF-A in human dermal microvascular endothelial cells from 46.9 to 35.6 pg/mL compared to untreated control. A 76.6% tumor regression was recorded in a lung tumor xenograft mouse model following intervention with CLA-coated PTX-SPIONs@HRH, demonstrating tumor targetability and angiogenesis inhibition. CLA-coated PTX-SPIONs@HRH enhanced the half-life of PTX by almost 2-folds and demonstrated a prolonged PTX plasma circulation time from a subcutaneous injection (SC). Thus, it is suggested that CLA-coated PTX-SPIONs@HRH could provide a potential effective treatment modality for non-small-cell lung carcinoma as a nanomedicine.

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Section: Resultsmentioning

confidence: 99%

Surface Immobilization of Anti-VEGF Peptide on SPIONs for Antiangiogenic and Targeted Delivery of Paclitaxel in Non-Small-Cell Lung Carcinoma

Ngema

Adeyemi

Marimuthu

et al. 2023

ACS Appl. Bio Mater.

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“…In essence, an increase in tumor size should account for a reduced total body weight due to weight loss from cancer cachexia [ 56 , 57 , 58 ]. As such, the control group with a high tumor volume displayed a reduced total body weight (29.20–22.96 g) compared with the treated group ( Figure 11 ) ( p < 0.05).…”

Section: Resultsmentioning

confidence: 99%

“…More importantly, the ENT-loaded LyP-1-modified nanosystem provided targeted drug delivery with superior anti-tumor effects of ENT to potentially treat OSCC. In essence, an increase in tumor size should account for a reduced total body weight due to weight loss from cancer cachexia [56][57][58]. As such, the control group with a high tumor volume displayed a reduced total body weight (29.20-22.96 g) compared with the treated group (Figure 11) (p < 0.05).…”

Section: Effects On Tumor Volume and Mouse Weightmentioning

confidence: 97%

In Vitro and In Vivo Evaluation of a Cyclic LyP-1-Modified Nanosystem for Targeted Endostatin Delivery in a KYSE-30 Cell Xenograft Athymic Nude Mice Model

Adeyemi

Choonara

2022

Pharmaceuticals

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This work investigated the use of LyP-1 as a homing peptide for p32 receptor targeting on the surface of an endostatin (ENT)-loaded chitosan-grafted nanosystem intended for intracellular delivery of ENT and mitochondrial targeting in a squamous cell carcinoma (SCC) cell line (KYSE-30) model. The angiogenic factors for VEGF-C and MMP2 were assessed with in vivo evaluation of the nanosystem upon ENT release and tumor necrosis in nude mice with a KYSE-30 cell xenograft. The LyP-1-modified nanosystem revealed a three-fold decrease in proliferation at 1000 µg/mL compared with the control and facilitated receptor-mediated cellular uptake and internalization. In addition, targeting of the Lyp-1-functionalized nanosystem to mitochondrial and nuclear proteins in vitro and in vivo was achieved. Up to 60% inhibition of KYSE-30 cell migration was observed and the expressions of VEGF-C and MMP-2 as angiogenic markers were reduced 3- and 2-fold, respectively. A marked reduction in tumor mass was recorded (43.25%) with the control, a 41.36% decrease with the nanoparticles and a 61.01% reduction with the LyP-1-modified nanosystem following treatment in mice. The LyP-1-functionalized nanosystem targeted tumor lymphatics, instigated nuclear rupture and mitochondrial distortion, and decreased cell proliferation and migration with inhibition of VEGF-C and MMP2 expression.

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“…Since handling may affect animal distress, the distress score was assessed before weighting. The distress score sheet comprises body weight, general condition, spontaneous behaviour, flight behaviour and process-specific criteria, as previously published 40 . (Suppl.…”

Section: Methodsmentioning

confidence: 99%

3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress

Xie

Lorenz

Poosch

et al. 2022

Sci Rep

Self Cite

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The dorsal skinfold chamber is one of the most important in vivo models for repetitive longitudinal assessment of microcirculation and inflammation. This study aimed to refine this model by introducing a new lightweight chamber made from polyetheretherketone (PEEK). Body weight, burrowing activity, distress, faecal corticosterone metabolites and the tilting angle of the chambers were analysed in mice carrying either a standard titanium chamber or a PEEK chamber. Data was obtained before chamber preparation and over a postoperative period of three weeks. In the early postoperative phase, reduced body weight and increased faecal corticosterone metabolites were found in mice with titanium chambers. Chamber tilting and tilting-related complications were reduced in mice with PEEK chambers. The distress score was significantly increased in both groups after chamber preparation, but only returned to preoperative values in mice with PEEK chambers. In summary, we have shown that light chambers reduce animal distress and may extend the maximum dorsal skinfold chamber observation time. Chambers made of PEEK are particularly suitable for this purpose: They are autoclavable, sufficiently stable to withstand rodent bites, inexpensive, and widely available through 3D printing.

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Diagnostic Ability of Methods Depicting Distress of Tumor-Bearing Mice

Cited by 3 publications

References 37 publications

Surface Immobilization of Anti-VEGF Peptide on SPIONs for Antiangiogenic and Targeted Delivery of Paclitaxel in Non-Small-Cell Lung Carcinoma

Surface Immobilization of Anti-VEGF Peptide on SPIONs for Antiangiogenic and Targeted Delivery of Paclitaxel in Non-Small-Cell Lung Carcinoma

In Vitro and In Vivo Evaluation of a Cyclic LyP-1-Modified Nanosystem for Targeted Endostatin Delivery in a KYSE-30 Cell Xenograft Athymic Nude Mice Model

3D-printed lightweight dorsal skin fold chambers from PEEK reduce chamber-related animal distress

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