Diagnosis of Tuberculous Lymphadenitis in Butajira, Rural Ethiopia

Yassin, Mohammed A.; Olobo, Joseph; Kidane, Dawit; Negesse, Yohannes; Shimeles, Ezra; Tadesse, Azeb; Demissie, Abebech; Britton, Sven; Harboe, Morten; Aseffa, Abraham; Abate, Getahun

doi:10.1080/00365540310004027

Cited by 35 publications

(36 citation statements)

References 12 publications

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“…The presence of granulomas in 24 of 49 culture-negative patients might be due to infection by either tuberculous or nontuberculous mycobacteria (NTM) which were not adequately detected by the sample culture. Our results are compatible with a study conducted in Butajira, Southern Ethiopia, which reported that in 23 patients identified with nontuberculous lymphadenitis by cytology, PCR confirmed the presence of MTC in 15 and in the remaining 8, MTC was not detected [21]. Moreover, a recent study conducted at AHRI using FNAs and biopsies of 117 TBLN patients from eastern, central and northern Ethiopia identified only M. tuberculosis as the etiological agent of TBLN by using the RD10 primers system [22].…”

Section: Discussionsupporting

confidence: 82%

Identification and genotyping of the etiological agent of tuberculous lymphadenitis in Ethiopia

Beyene

Bergval

Hailu

et al. 2009

J Infect Dev Ctries

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Background: In Ethiopia, little has been done to assess how Mycobacterium bovis has contributed to human tuberculosis, though the population routinely consumes unpasteurized milk and raw meat. The aim of this study was to determine the proportion of M. tuberculosis and M. bovis as etiological agents of tuberculous lymphadenitis (TBLN). Methods: Patients with lymphadenopathy (n = 171) were included in a cross-sectional study at Butajira Hospital, Southern Ethiopia. Lymph node biopsies were cultured. Patients' HIV status was identified. DNA from positive cultures was tested by PCR to identify M. bovis and M. tuberculosis. Isolates were genotyped by multiplex ligation-dependent probe amplification (MLPA) assay. Results: Among 171 patients, 156 had culture results. Of these, 107 (69%) were positive for M. tuberculosis complex (MTC). Six of the 10 HIV-positive patients were culture positive. M. tuberculosis specific sequences were identified in the DNA of each of 100 samples as assessed by RD10 targeted PCR, and each of the 95 isolates exhibited the M. tuberculosis specific TbD1 deletion by MLPA analysis. No M. bovis was identified. These results indicate that all the isolates were modern M. tuberculosis strains. Furthermore, MLPA studies confirmed that 42% of the isolates showed the Haarlem genotype and 12% displayed sequences compatible with INH resistance. No mutations conferring resistance to ethambutol or rifampicin were detected. Conclusions: Our data showed that M. tuberculosis strains had common characteristics with strains causing pulmonary TB, which appears to be the main etiological agent of TBLN.

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Section: Discussionsupporting

confidence: 82%

Identification and genotyping of the etiological agent of tuberculous lymphadenitis in Ethiopia

Beyene

Bergval

Hailu

et al. 2009

J Infect Dev Ctries

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“…32,33 Expertise in excisional biopsy is often limited in developing countries. 34 Histology of excisional biopsy shows in over 90% evidence of caseous granulomas. 35 Culture of biopsy samples have shown positive culture rates in 77-90%.…”

Section: Tuberculous Lymphadenitismentioning

confidence: 99%

“…Culture alone was relied upon in seven studies (44%) and culture plus anti-T therapy treatment, histology or X-ray in six studies. The remaining two did not include culture as part of the reference standard but relied on cytology alone 34 or histology plus an anti-TB therapy trial. 217 …”

Section: Reference Standardsmentioning

confidence: 99%

A systematic review of rapid diagnostic tests for the detection of tuberculosis infection

Dinnes¹,

Deeks²,

Kunst³

et al. 2007

Health Technol Assess

529

361

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“…This observation was comparable to finding of a study where 67.5% cervical, 9% axillary and 6.4% inguinal lymph node involvements were reported 15 . A study in Butajira, Ethiopia, revealed that 88(82.2%) of 105 tuberculous lymphadenitis cases had cervical involvement 16 .…”

Section: Discussionmentioning

confidence: 99%