Diagnosis of tuberculosis in an Indian population by an indirect ELISA protocol based on detection of Antigen 85 complex: a prospective cohort study

Kashyap, Rajpal S.; Rajan, A N; Ramteke, Suman; Agrawal, Vijay S; Kelkar, Sanjivani S; Purohit, Hemant J.; Taori, Girdhar M.; Daginawala, Hatim F.

doi:10.1186/1471-2334-7-74

Cited by 63 publications

(54 citation statements)

References 20 publications

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“…A monoclonal antibody was also generated against the Ag85c and the antigen was found in the sputum of patients with PTB (Wallis et al 1998) and in patients with EPTB. A study performed using cerebrospinal fluid indicated that an indirect ELISA test using monoclonal antibodies against the Ag85c yielded 82% sensitivity and 86% specificity (Kashyap et al 2007). In this study, the Ag85c test (Ag85c) reached 85% sensitivity and 77.5% specificity and the Ag85A peptide tests achieved better sensitivity and specificity when compared to Ag85c: peptides 11005 and 11006 had a sensitivity of 98.3% for the EPTB group and peptide 29878 exhibited specificities of 97.4% and 96.4%, for the PTB and EPTB groups, respectively.…”

Section: Discussionmentioning

confidence: 99%

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

Araujo¹,

Giampietro²,

Bochichio³

et al. 2013

Mem. Inst. Oswaldo Cruz

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The World Health Organization (WHO) lists tuberculosis (TB) as the most important fatal infection worldwide (WHO 2009). The development of a new diagnostic test for TB infection is an important component of the Global Plan to Stop TB and the WHO Stop TB Strategy. In 2005 alone, an estimated 8.8 million individuals were infected with TB and 1.6 million people died of the disease (WHO 2009). Importantly, less than one half of the total 8.8 million estimated cases were diagnosed as smear-positive; the diagnosis of smear-negative patients has proven to be more challenging. Currently, a definitive diagnosis of both pulmonary TB (PTB) and extrapulmonary TB (EPTB) relies on the time consuming culture of mycobacteria. A vast majority of TB cases occur in developing countries that have limited resources. Rapid, inexpensive diagnostic tests would aid these countries in limiting the spread of infection within their communities (WHO 2009). In addition, molecular methods based on nucleic acid amplification to diagnose TB infection are rapid, highly specific and more sensitive than microscopic examination of smears, but are less sensitive compared to culture assays for smear-negative TB cases (Abebe et al. 2007).Serological tests that rely on the detection of antibodies against Mycobacterium tuberculosis-specific antigens possess several advantages: they are simple, inexpensive and feasible for the diagnosis of TB. Potential M. tuberculosis antigens were recently reviewed in a metaanalysis. A total of 254 studies were identified that encompassed nine native proteins, 27 recombinant proteins, 15 lipid-derived antigens and 30 combination antigen targets. These results indicated that highly specific tests frequently exhibited poor sensitivity, which limited the use of these antigens when a single antigen was used in the assay (Steingart et al. 2009). Recently, there has been renewed interest in the development of antibody-based diagnostic assays that utilise multiple antigens to achieve high sensitivity and specificity (Abebe et al. 2007). Many attempts to develop a serologic TB test have been made. These assays need to discriminate active from latent infection, avoid cross-reactivity with Bacillus Calmette-

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Section: Discussionmentioning

confidence: 99%

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

Araujo¹,

Giampietro²,

Bochichio³

et al. 2013

Mem. Inst. Oswaldo Cruz

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“…Simultaneously in the year 2007, monoclonal antibodies against antigen 85 complex have been used for serodiagnosis by indirect ELISA method [16]. LED based fluorescence microscopy has been reported to be 10% more sensitive than ZN microscopy [16].…”

Section: Proteins Released By M Tb Bacilli During Growthmentioning

confidence: 99%

“…LED based fluorescence microscopy has been reported to be 10% more sensitive than ZN microscopy [16]. The transformation in TB diagnosis was brought about with the introduction of Genexpert test, which diagnoses TB by detecting the presence of TB bacteria, as well as testing for resistance to the drug Rifampicin [17].…”

Section: Proteins Released By M Tb Bacilli During Growthmentioning

confidence: 99%

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Waghmare¹,

Wankhade²,

Jena³

et al. 2016

Mycobact Dis

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TB immunodiagnostics based on antibody and antigen detection for early detection and monitoring tubercular infections at low cost and flexible to adapt to field laboratories are a boon to developing countries. In this context we have explored in-house developed penicillinase based ELISA assays for the detection of antigen, antibody as well as immune-complexed antigen using various excretory secretory antigenic proteins and specific antibodies. Excretory secretory protein antigens such as ES-31, ES-41, ES-43, ES-6, ES-20, ES-100 and EST-6 were studied extensively and found to be useful in various pulmonary and extra pulmonary cases of tuberculosis infection. ES-31 has shown its diagnostic potential in chronic PTB cases, ES-43 in relapse cases and ES-41 in bone and joint TB. Elevated level of ES-20 antigen was observed in patients with weak immune response in TB lymphadenitis. Detection of ES-100 antigen and antibody by penicilinase ELISA was observed to be useful in detection of TB meningitis. ES-6 antigen was shown to be useful in detection of latent infection. These proteins with antigenic properties are utilized for production of specific antibodies against them and observed to be useful in immune diagnostics for detection of specific circulating and immune complexed antigens. Further user friendly peroxidase immunoassay has been standardised and is being routinely used for suspected TB cases attending 1000 bedded Kasturba Hospital attached to medical institute on physician's request.

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“…Antigen85 complex and CFP M. tuberculosis elicits detectable IgG response in tuberculosis patients. Sensitivity and specificity of assays utilizing Ag85 complex have been reported to range from 82-84.1% and 85.2-86% respectively [8]. Serological tests detecting IgG antibody against CFP showed sensitivity and specificity of 60.4 to 66% and 73.8 to 85.2% respectively [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Humoral immune response to selective mycobacterial antigens and serodiagnosis of active tuberculosis in Bangladeshi patients

Mohiuddin¹,

Haq²

2017

IMC J Med Sci

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Background and objective: Serological test has become an important tool in the diagnosis of TB cases. This study focused on the analysis and comparison of antibody response to two Mycobacterium tuberculosis (MTB) antigens namely Ag85 complex and culture filtrate protein (CFP) in patients with tuberculosis. Antibody response to specific antigen was utilized as a diagnostic tool to detect active tuberculosis (TB) cases.

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Diagnosis of tuberculosis in an Indian population by an indirect ELISA protocol based on detection of Antigen 85 complex: a prospective cohort study

Cited by 63 publications

References 20 publications

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

Excretory Secretory Proteins Released during Growth of Mycobacterium tuberculosis (H37Ra), With Diagnostic Potential in Pulmonary and Extra Pulmonary Tuberculosis

Humoral immune response to selective mycobacterial antigens and serodiagnosis of active tuberculosis in Bangladeshi patients

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