Diagnosis of Trichomonas vaginalis in adolescent females: ‘Inpouch TV’ culture versus wet mount microscopy

Ohlemeyer, Chris L.; Hornberger, Laurie L.; Lynch, Daryl A.; Swierkosz, Ella M.

doi:10.1016/1054-139x(96)81151-7

Cited by 9 publications

(12 citation statements)

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“…2,15,[21][22][23][24] When compared to IP, the reference test, the sensitivity of WM in the present study was 67%, consistent with other published studies comparing these 2 testing methodologies in sexually active adolescent and adult females. 11,12 As previously mentioned, studies evaluating STIs in children often used WM as the only diagnostic method and confined testing to those girls with vaginal discharge. [13][14][15] Although it may be intuitive that the rate of recovery for TV is greater in the presence of vaginal discharge, 42% (5/12) of these patients in whom TV was diagnosed had no vaginal discharge (P !…”

Section: Discussionmentioning

confidence: 97%

“…Advantages of IP in comparison with Diamond's medium include lower cost, less strict storage and transportation requirements, and decreased time and preparation. 12 Additionally, IP does not promote the growth of Trichomonas hominis.…”

Section: Introductionmentioning

confidence: 99%

“…However, comparative studies of WM and culture in sexually active adolescent and adult females have demonstrated the sensitivity of WM in the detection of TV to be notably less (36%-75%) than the sensitivity of culture. 8,[10][11][12] Ohlemeyer and colleagues compared WM to 2 culture methods, InPouch TV (IP; Biomed Diagnostics, San Jose, CA) and Diamond's medium, and noted that the sensitivity of IP (81%) exceeded that of WM (36%) using Diamond's as the ''gold standard.'' 12 They concluded that IP was a superior method of diagnosing TV because its sensitivity approached that of Diamond's medium.…”

Section: Introductionmentioning

confidence: 99%

“…8,[10][11][12] Ohlemeyer and colleagues compared WM to 2 culture methods, InPouch TV (IP; Biomed Diagnostics, San Jose, CA) and Diamond's medium, and noted that the sensitivity of IP (81%) exceeded that of WM (36%) using Diamond's as the ''gold standard.'' 12 They concluded that IP was a superior method of diagnosing TV because its sensitivity approached that of Diamond's medium. Advantages of IP in comparison with Diamond's medium include lower cost, less strict storage and transportation requirements, and decreased time and preparation.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diagnosis of Trichomonas vaginalis in Female Children and Adolescents Evaluated for Possible Sexual Abuse: A Comparison of the InPouch TV Culture Method and Wet Mount Microscopy

Gallion

Dupree²,

Scott

et al. 2009

Journal of Pediatric and Adolescent Gynecology

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Diagnosis of Trichomonas vaginalis in Female Children and Adolescents Evaluated for Possible Sexual Abuse: A Comparison of the InPouch TV Culture Method and Wet Mount Microscopy

Gallion

Dupree²,

Scott

et al. 2009

Journal of Pediatric and Adolescent Gynecology

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“…Culture for Trichomonas, currently considered the gold standard diagnostic method, may be performed by inoculation of the vaginal swab sample into a medium specifically optimized for anaerobic growth of T. vaginalis with microscopic examination of the culture for up to 5 days. This process has been improved by the In-Pouch culture system (BioMed Diagnostics, San Jose, CA), which is a closed culture tube that allows visual inspection of the entire culture [17][18][19]. Of critical importance to understanding the existing literature regarding Trichomonas diagnosis is the fact that culture is often the reference method by which the performance of a diagnostic method is evaluated, and culture depends on capture of viable organisms and efficient clinical laboratory transportation systems.…”

Section: T Vaginalismentioning

confidence: 99%

Diagnosing Vaginal Infections: It’s Time to Join the 21st Century

Pol

2010

Curr Infect Dis Rep

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Vaginal infections are one of the most common reasons for medical healthcare visits in the United States and Western Europe. These diseases are also significantly associated with HIV acquisition in resource-constrained settings throughout the world. However, despite ample opportunity to improve diagnosis, and therefore management, of vaginitis and vaginosis, the healthcare community continues to rely on diagnostic methods with poor performance characteristics. This state of affairs results in part from the lack of a public health mandate to reduce the burden of these infections in women. Without such a mandate, there is a lack of funding for reimbursement for more sensitive and specific diagnostic assays. Sensitive assays do exist for these infections, and their use should be encouraged when appropriate for clinical care.

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Efficacy and Safety of Single Oral Dosing of Secnidazole for Trichomoniasis in Women: Results of a Phase 3, Randomized, Double-Blind, Placebo-Controlled, Delayed-Treatment Study

Muzny

Schwebke

Nyirjesy

et al. 2021

Clinical Infectious Diseases

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Background Trichomonas vaginalis is the most prevalent non-viral sexually transmitted infection. We evaluated the efficacy and safety of secnidazole vs. placebo in women with trichomoniasis. Methods Women with trichomoniasis, confirmed by a positive T. vaginalis culture, were randomized to single-dose oral secnidazole 2g or placebo. The primary endpoint was microbiological test of cure (TOC) by culture 6–12 days after dosing. At the TOC visit, participants were given the opposite treatment. They were followed for resolution of infection afterward and offered treatment at subsequent visits, if needed. Fifty patients per group (N=100) provided ~95% power to detect a statistically significant difference between treatment groups. Results Between April 2019 and March 2020, 147 women enrolled at 10 US sites. The modified intent-to-treat (mITT) population included 131 randomized patients (64/67, in secnidazole/placebo). Cure rates were significantly higher in the secnidazole vs. placebo group (92.2% [95% CI: 82.7–97.4] vs. 1.5% [95% CI: 0.0–8.0]) for the mITT population and for the per-protocol population (94.9% [95% CI: 85.9–98.9]) vs. 1.7% [95% CI: 0.0–8.9]). Cure rates were 100% (4/4) in women with HIV and 95.2% (20/21) in women with bacterial vaginosis (BV). Secnidazole was generally well tolerated. The most frequently reported treatment-emergent adverse events (TEAEs) were vulvovaginal candidiasis and nausea (each 2.7%). No serious TEAEs were observed. Conclusion A single oral 2g dose of secnidazole was associated with significantly higher microbiological cure rates vs. placebo, supporting a role for secnidazole in treating women with trichomoniasis, including those with HIV and/or BV.

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Diagnosis of Trichomonas vaginalis in adolescent females: ‘Inpouch TV’ culture versus wet mount microscopy

Cited by 9 publications

References 0 publications

Diagnosis of Trichomonas vaginalis in Female Children and Adolescents Evaluated for Possible Sexual Abuse: A Comparison of the InPouch TV Culture Method and Wet Mount Microscopy

Diagnosis of Trichomonas vaginalis in Female Children and Adolescents Evaluated for Possible Sexual Abuse: A Comparison of the InPouch TV Culture Method and Wet Mount Microscopy

Diagnosing Vaginal Infections: It’s Time to Join the 21st Century

Efficacy and Safety of Single Oral Dosing of Secnidazole for Trichomoniasis in Women: Results of a Phase 3, Randomized, Double-Blind, Placebo-Controlled, Delayed-Treatment Study

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