Diagnosis of Toxoplasma gondii infection in pregnant women using automated chemiluminescence and quantitative real time PCR

Ahmadpour, Ehsan; Zargami, Elmira; Mahami-Oskouei, Mahmoud; Spotin, Adel; Shahbazi, A; Kafil, Hossein Samadi; Rajabi, Saba; Alizadeh, Paria; Azadi, Yagoob; Bahaj, Reza; Shahrivar, Firooz; Barać, Aleksandra

doi:10.4103/1995-7645.250341

Cited by 6 publications

(4 citation statements)

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“…Toxoplasmosis is considered as a global health problem with nearly a billion people infected, reaching a prevalence of the disease in 71.5% ( Ahmadpour et al., 2019 ). Regarding the treatment, a combination of sulfadiazine and pyrimethamine (SDZ + PYR) is the first choice to treat congenital toxoplasmosis, one of the most serious forms of the disease ( Abugri et al., 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Leaf hydroalcoholic extract and oleoresin from Copaifera multijuga control Toxoplasma gondii infection in human trophoblast cells and placental explants from third-trimester pregnancy

Martínez

Teixeira²,

Souza³

et al. 2023

Front. Cell. Infect. Microbiol.

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The conventional treatment of congenital toxoplasmosis is mainly based on the combination of sulfadiazine and pyrimethamine. However, therapy with these drugs is associated with severe side effects and resistance, requiring the study of new therapeutic strategies. There are currently many studies with natural products, including Copaifera oleoresin, showing actions against some pathogens, as Trypanosoma cruzi and Leishmania. In the present study, we investigated the effects of the leaf hydroalcoholic extract and oleoresin from Copaifera multijuga against Toxoplasma gondii in human villous (BeWo) and extravillous (HTR8/SVneo) trophoblast cells, as well as in human villous explants from third-trimester pregnancy. For this purpose, both cells and villous explants were infected or not with T. gondii, treated with hydroalcoholic extract or oleoresin from C. multijuga and analyzed for toxicity, parasite proliferation, cytokine and ROS production. In parallel, both cells were infected by tachyzoites pretreated with hydroalcoholic extract or oleoresin, and adhesion, invasion and replication of the parasite were observed. Our results showed that the extract and oleoresin did not trigger toxicity in small concentrations and were able to reduce the T. gondii intracellular proliferation in cells previously infected. Also, the hydroalcoholic extract and oleoresin demonstrated an irreversible antiparasitic action in BeWo and HTR8/SVneo cells. Next, adhesion, invasion and replication of T. gondii were dampened when BeWo or HTR8/SVneo cells were infected with pretreated tachyzoites. Finally, infected and treated BeWo cells upregulated IL-6 and downmodulated IL-8, while HTR8/SVneo cells did not change significantly these cytokines when infected and treated. Finally, both the extract and oleoresin reduced the T. gondii proliferation in human explants, and no significant changes were observed in relation to cytokine production. Thus, compounds from C. multijuga presented different antiparasitic activities that were dependent on the experimental model, being the direct action on tachyzoites a common mechanism operating in both cells and villi. Considering all these parameters, the hydroalcoholic extract and oleoresin from C. multijuga can be a target for the establishment of new therapeutic strategy for congenital toxoplasmosis.

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Section: Discussionmentioning

confidence: 99%

Leaf hydroalcoholic extract and oleoresin from Copaifera multijuga control Toxoplasma gondii infection in human trophoblast cells and placental explants from third-trimester pregnancy

Martínez

Teixeira²,

Souza³

et al. 2023

Front. Cell. Infect. Microbiol.

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“…Molecular methods are efficient techniques that allow specific amplification of DNA. The real-time qPCR could be successfully used to T. gondii diagnosis and is capable to detect low concentrations of target DNA [3,22,32]. Besides, to increase the specificity and sensitivity of immunological assays in immunocompromised patients, new antigenic targets should be designed the parasite to have a potential immunogenic antigen in immunodiagnostic tools of toxoplasmosis in patients with cancer [5].…”

Section: Discussionmentioning

confidence: 99%

“…Genomic DNA was extracted from the isolated buffy coat samples using DNA extraction mini kit (YTA Co., Iran) according to the manufacturer's instruction. Specific primers for repeated element (RE) gene of T. gondii (F 5'-AGG GAC AGA AGT CGA AGG GG-3' and R 5'-GCA GCC AAG CCG GAA ACA TC-3') was designed using NCBI blast software [3]. The DNA samples were tested for the presence of the 164-bp fragment of RE gene of T. gondii by realtime PCR machine (Rotor-Gene Q, Qiagen, USA), in 20 μl volumes, as previously described [3].…”

Section: Methodsmentioning

confidence: 99%

“…Specific primers for repeated element (RE) gene of T. gondii (F 5'-AGG GAC AGA AGT CGA AGG GG-3' and R 5'-GCA GCC AAG CCG GAA ACA TC-3') was designed using NCBI blast software [3]. The DNA samples were tested for the presence of the 164-bp fragment of RE gene of T. gondii by realtime PCR machine (Rotor-Gene Q, Qiagen, USA), in 20 μl volumes, as previously described [3]. Briefly, 1 μg of DNA template was added to SYBR Green PCR Master Mix (YTA Co., Iran).…”

Section: Methodsmentioning

confidence: 99%

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<i>Toxoplasma gondii</i> infection in patients with malignant and benign bone tumours

Hajizadeh

Falak

Tavakoli

et al. 2021

Russian Journal of Infection and Immunity

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Toxoplasma gondii (T. gondii) is an intracellular parasite that infects humans and seroprevalence of its infection varies from about 10 to 80 percent in different countries with a higher prevalence in warmer and humid regions. In this study, the rate of acute and chronic toxoplasmosis in patients with benign or malignant bone tumours was investigated. Fifty-three patients who suffered from various bone tumours, as well as sixty-five healthy controls with an unknown serological profile for anti-Toxoplasma antibodies, were enrolled in this cross-sectional study. Anti-toxoplasma antibodies were detected in serum samples using enzyme-linked immunosorbent assay (ELISA) and blood samples of them were used for real-time PCR. Thirty-two (60.32%) and twenty-one (39.63%) of patients had malignant tumours and benign tumours, respectively. The results showed a higher and significant seropositivity rate of IgM antibodies in primary bone tumour patients compared to the control group and Toxoplasma DNA became positive in 18.86% of patients with primary bone tumours and 6.15% of controls. Surprisingly, the high presence of parasite DNA was detected in patients with malignant tumours. The seroprevalence of T. gondii IgM antibody and DNA positivity among the cancer patients were significantly higher than healthy individuals. Also, chronic toxoplasmosis (it was shown with IgG positive) appears to be more common in people with benign cancers than malignancies. The study showed a relatively high seroprevalence of anti-T. gondii antibodies in patients with primary bone cancer. However, the considerable rate of positive blood samples for the presence of parasite’s DNA should not be ignored. A key to the effective management of diseases in immunosuppressed individuals is prompt and accurate diagnosis of toxoplasmosis. Moreover, it seems that PCR tests may be more reliable than serological methods and it could be considered as a precise method for diagnosis of acute toxoplasmosis.

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A novel enhanced dot blot immunoassay using colorimetric biosensor for detection of Toxoplasma gondii infection

Safarpour

Pourhassan‐Moghaddam

Spotin

et al. 2021

Comparative Immunology, Microbiology and Infectious Diseases

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Diagnosis of Toxoplasma gondii infection in pregnant women using automated chemiluminescence and quantitative real time PCR

Cited by 6 publications

References 1 publication

Leaf hydroalcoholic extract and oleoresin from Copaifera multijuga control Toxoplasma gondii infection in human trophoblast cells and placental explants from third-trimester pregnancy

Leaf hydroalcoholic extract and oleoresin from Copaifera multijuga control Toxoplasma gondii infection in human trophoblast cells and placental explants from third-trimester pregnancy

<i>Toxoplasma gondii</i> infection in patients with malignant and benign bone tumours

A novel enhanced dot blot immunoassay using colorimetric biosensor for detection of Toxoplasma gondii infection

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