Diagnosis of the pinwormSyphacia murisin the Wistar ratRattus norvegicus

Abstract

This study aimed to compare three qualitative parasitological methods for the diagnosis of Syphacia muris infection in 30 Wistar rats (Rattus norvegicus) infected naturally. Methods of spontaneous sedimentation (Hoffman, Pons and Janer, or HPJ) and spontaneous flotation (Willis) for faecal samples and a method of taping (Graham) were performed and compared. The Graham and Willis methods were more sensitive than the HPJ method (P< 0.05). The Graham method was able to detect S. muris eggs in 100% of the samples. Eggs were detected in 83% and 60% of the samples using the Willis and HPJ methods, respectively. Method choice is important for screening for parasites of rats kept under laboratory conditions, as accurate diagnosis helps prevent future environmental contamination and infection. We concluded that the Graham method was the most efficient of those tested in this study for detection of S. muris infection in rats. This method is also rapid, inexpensive and practical, and should be implemented as a necessary measure for infection control.

Veterinary monitoring of gastrointestinal parasites in European bison, Bison bonasus designated for translocation: Comparison of two coprological methods

“…Veterinary practice has at its disposal a variety of coprological techniques for the diagnosis of internal parasitic infections; one such tool is the flotation method, which is simple, quick, inexpensive and can be easy applied in the field ( Pouillevet et al, 2017 ; Jolles et al, 2008 ; Sousa et al, 2016 ). However, this wide variety of methods limits the possibility for comparison of coprological results between different scenarios.…”

Molecular identification and subtyping of Blastocystis sp. in laboratory rats in China

“…Parasite 27, 35 (2020) Ó J. Li et al, published by EDP Sciences, 2020 https://doi.org/10.1051/parasite/2020035 Available online at: www.parasite-journal.org reaction conditions [8]. Infections with additional pathogens, such as unrelated parasites, can be a persistent constraint for animal breeding and experimentation due to developmental interference, which may impact the progress of biomedical research [10,19]. Blastocystis sp.…”

<b>Caracterização anatomopatológica, microbiológica e parasitológica de ratos Wistar (<i>Rattus norvegicus</i>) em diferentes idades</b>

“…O programa de erradicação será eficiente quando associar protocolo terapêutico baseado em anti-helmínticos (ZENNER;JOHNSTON, 2002;BAKER, 2007;MOREIRA et al, 2013), e limpeza e desinfeção das instalações e gaiolas (PRITCHETT; JOHNSTON, 2002;DIX;ASTILL;WHELAN, 2004;MOREIRA et al, 2013;MEADE;WATSON, 2014). Porém, este protocolo é trabalhoso e de elevado custo e não elimina a possibilidade de reinfecção (ZENNER;SOUSA et al, 2014). A forma mais eficaz para a erradicação total deste parasito é a formação de uma nova colônia, com animais obtidos por técnicas de rederivação cesariana ou transferência embrionária (ZENNER;JOHNSTON, 2002).…”