Diagnosis of Sarcoidosis

Parrish, Scott; Turner, J. Francis

doi:10.1016/j.disamonth.2009.06.001

Cited by 18 publications

(29 citation statements)

References 36 publications

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“…The diagnosis of sarcoidosis was made on the basis of the compatible clinical pictures and histologic demonstration of noncaseating granulomas [12, 13] The diagnosis of HP and sarcoidosis needed exclusion of other diseases capable of producing a similar histologic picture: Biopsy tissues were subjected to special stains (acid fast bacilli stain and Gömöri methenamine silver stain) to rule out microorganisms and fungi. IPF patients were evaluated using serial FVC and DLCO measurements.…”

Section: Methodsmentioning

confidence: 99%

Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

et al. 2017

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BackgroundIdiopathic pulmonary fibrosis (IPF) is characterized by the complex interaction of cells involved in chronic inflammation and fibrosis. Global gene expression of a homogenous cell population will identify novel candidate genes.MethodsGene expression of fibroblasts derived from lung tissues (8 IPF and 4 controls) was profiled, and ontology and functional pathway were analyzed in the genes exhibiting >2 absolute fold changes with p-values < 0.05. CCL8 mRNA and protein levels were quantified using real-time PCR and ELISA. CCL8 localization was evaluated by immunofluorescence staining.ResultsOne hundred seventy eight genes differentially expressed and 15 genes exhibited >10-fold change. Among them, 13 were novel in relation with IPF. CCL8 expression was 22.8-fold higher in IPF fibroblasts. The levels of CCL8 mRNA and protein were 3 and 9-fold higher in 14 IPF fibroblasts than those in 10 control fibroblasts by real-time PCR and ELISA (p = 0.022 and p = 0.026, respectively). The CCL8 concentrations in BAL fluid was significantly higher in 86 patients with IPF than those in 41 controls, and other interstitial lung diseases including non-specific interstitial pneumonia (n = 22), hypersensitivity pneumonitis (n = 20) and sarcoidosis (n = 19) (p < 0.005, respectively). Cut-off values of 2.29 pg/mL and 0.43 pg/mL possessed 80.2 and 70.7% accuracy for the discrimination of IPF from NC and the other lung diseases, respectively. IPF subjects with CCL8 levels >28.61 pg/mL showed shorter survival compared to those with lower levels (p = 0.012). CCL8 was expressed by α-SMA-positive cells in the interstitium of IPF.ConclusionsTranscriptome analysis identified several novel IPF-related genes. Among them, CCL8 is a candidate molecule for the differential diagnosis and prediction of survival.Electronic supplementary materialThe online version of this article (doi:10.1186/s12931-016-0493-6) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Gene profile of fibroblasts identify relation of CCL8 with idiopathic pulmonary fibrosis

et al. 2017

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“…A medical centre that performed[10,000 Kveim-Siltzbach tests over 50 years identified a true positive rate of[50% and false negative rate of nearly zero (Iannuzzi et al 2007). This test is now less often used since no commercially available preparation of the reagent exists and the problem that each new Kveim-Siltzbach preparation requires validation in vivo (Parrish and Turner 2009). Furthermore, use of human tissue extracts for clinical purposes presents constraints, including risks of transmitting infections such as Creutzfeldt-Jakob disease even after heating, phenol and irradiation (de Silva and Will 1993).…”

Section: The Kveim-siltzbach Testmentioning

confidence: 98%

“…It was performed by intradermal injection of Kveim reagent, a validated suspension of human sarcoid tissue, typically sarcoid spleen homogenised in phosphate-buffered saline (Siltzbach 1961;Teirstein 1986). The resultant papule at the injection site was biopsied 4 weeks later and presence of non-caseating granulomas indicated sarcoidosis (Kveim 1941;Chase 1961;Parrish and Turner 2009). In 1967 Siltzbach demonstrated that sarcoid patients worldwide reacted positively to a single batch of Kveim reagent indicating the possibility of a common antigen in the aetiology of sarcoidosis (Siltzbach 1967).…”

Section: The Kveim-siltzbach Testmentioning

confidence: 98%

“…BAL fluid has high positive predictive values for the diagnosis of pulmonary sarcoidosis by detecting a lymphocytosis with elevated ratios of CD4?/CD8? cells typically [3.5:1, in the absence of other causes (Winterbauer et al 1977;Parrish and Turner 2009). Analysis of T lymphocyte subsets and cytokine levels from BAL fluid and peripheral blood using flow cytometry have been compared (Iida et al 1997;Jamaati et al 2003).…”

Section: Immunopathogenesis Of Sarcoidosismentioning

confidence: 98%

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The potential of the immunological markers of sarcoidosis in exhaled breath and peripheral blood as future diagnostic and monitoring techniques

2011

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Sarcoidosis is characterised by non-caseating granulomatous inflammation, with exaggerated immune responses at sites of disease and derangements of normal tissue architecture. The lungs are most commonly involved and progressive inflammation may result in pulmonary fibrosis. The immunopathogenesis and aetiology remain uncertain, which has made it difficult to identify a single sufficiently sensitive or specific diagnostic marker. Further investigation is needed to identify sensitive and specific markers of disease, such as in peripheral blood and exhaled breath condensate (EBC). Identification of disease markers may also be useful for investigating disease activity and predicting progression to fibrosis. This review explores the literature on the cytokine profiles of blood and bronchoalveolar lavage lymphocytes following ex vivo stimulation, as well as disease markers measured using the medium of EBC.

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“…Sarcoidosis is a chronic multisystem inflammatory granulomatous disease. The etiology of sarcoidosis is unknown, but it is believed to be due to immunodysregulation . Sarcoidosis can be insidious and develop over several years.…”

Section: Introductionmentioning

confidence: 99%