Diagnosis of progressive multifocal leucoencephalopathy by PCR detection of JC virus from CSF

Brouqui, P.; Bollet, C.; Delmont, J.; Bourgeade, A.

doi:10.1016/0140-6736(92)90790-a

Cited by 23 publications

(8 citation statements)

References 6 publications

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“…From these studies it appears likely that BKV activation in the urinary tract under AIDS is correlated with the state of immunoincompetence. Contrasting reports with 6% BKV viruria in AIDS and AIDS/PML patients as detected by nested PCR technique could probably be explained by technical differences between laboratories (Brouqui et al, 1992;Ferrante et al, 1997).…”

Section: Activation Of Bkv Infection In the Urogenital Tractmentioning

confidence: 59%

Latent and Persistent Polyomavirus Infection

Dörries

2001

Human Polyomaviruses

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Section: Activation Of Bkv Infection In the Urogenital Tractmentioning

confidence: 59%

Latent and Persistent Polyomavirus Infection

Dörries

2001

Human Polyomaviruses

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“…After this reaction, the viruses are differentiated by digestion with the Barn HI restriction enzyme, which cleaves only the amplified JCV but not the BKV DNA and by DEIA. Several studies have already been conducted on the detection of human polyomavirus DNA in samples ranging from urine, cerebrospinal fluid (CSF) and brain tissue, using the single-step PCR Henson et al, 1991;Telenti et al, 19921, while others have employed the n-PCR to detect JCV in CSF and in brain autopsy tissue specimens of PML patients [Brouqui et al, 1992;Gibson et al, 1993;Bogdanovic et al, 19941. As the single-step PCR was not sufficiently sensitive, particularly in the case of the paraffin-embedded and formalin-fixed brain tissue specimens, an attempt was made to enhance sensitivity by a n-PCR using two sets of primers, the specificity of which was verified by restriction with the Barn HI enzyme.…”

Section: Discussionmentioning

confidence: 99%

PCR detection of JC virus DNA in brain tissue from patients with and without progressive multifocal leukoencephalopathy

Ferrante

Caldarelli-Stefano

Omodeo-Zorini

et al. 1995

Journal of Medical Virology

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Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system, which is thought to be a result of the reactivation of JC virus (JCV), a human polyomavirus. The disease occurs in individuals with immunosuppression and in recent years there has been an increase in PML cases due to AIDS. A nested polymerase chain reaction (n-PCR) was employed to detect JCV and BK virus (BKV) DNA in brain tissue collected postmortem from 28 AIDS patients with PML and from 13 patients without PML, but with other diagnoses, including solid tumors, Alzheimer's disease, thromboembolism, myocardial infarction and acute cerebrovascular diseases. All 28 brain specimens from the patients with PML were positive for JCV DNA when tested by n-PCR and three of the latter were also positive for BKV DNA. These results were confirmed by an enzyme restriction analysis and a DNA hybridization assay. Interestingly, in this study, JCV DNA was also found in 6 brain tissue specimens from 4 subjects with diseases unrelated to PML or AIDS. All the brain specimens from the control group were negative for BKV DNA. The results confirm that the n-PCR is a useful tool for PML diagnosis. The presence of JCV DNA in the brain tissue of patients without PML is particularly important since it indicates that JCV could be latent in the brains of immunocompetent individuals. Moreover, detection of simultaneous presence of JCV and BKV in the brain tissue of the patients with PML demonstrates that BKV may also infect the human brain without causing any apparent neurological disease.

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“…Consequently, molecular DNA detection methods were applied for the demonstration of the virus in the CSF [52,53], In PML cases studied in our laboratory, detection of JCV DNA by radioactive Southern blot hybridizations almost always failed, although in 1 case faint radioactive signals in the position of 5kb JCV DNA demonstrated the presence of full-length genomic DNA in the CSF. This was confirmed by electron-microscopic visualization of virus-like particles after ultracentrifugation of CSF from PML patients [54], However, PCR is necessary to detect low concentrations of virus with a specificity comparable to that of Southern blot hybridizations and to render bet ter rates of detection [ 16,20,44,52,53,[55][56][57][58].…”

Section: Demonstration Ofpolyomavirus Dna In Csfmentioning

confidence: 96%

Virus-Host Interactions and Diagnosis of Human Polyomavirus-Associated Disease

Dörries

1996

Intervirology

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Persistent human polyomavirus infection is asymptomatic with limited states of virus production in the healthy individual, in immunocompromised patients, however, infection may lead to uncontrolled virus growth and fatal disease. Predominantly the CNS and in rare cases the urogenital tract are affected. During the AIDS epidemic, the number of cases is steadily increasing, thus dictating the need for an early and fast differential diagnosis. Virological diagnosis is essentially based on the detection of virus products in tissue or body fluids. Although sensitive and specific diagnostic techniques are available for the diagnosis of polyomavirus infection, widely accepted standards are not yet reached. This is in part due to the low number of cases in the past, but also to asymptomatic viral activation in nondiseased patients. Thus, additional parameters have to be evaluated to provide complementary tools for differential diagnosis.

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Diagnosis of progressive multifocal leucoencephalopathy by PCR detection of JC virus from CSF

Cited by 23 publications

References 6 publications

Latent and Persistent Polyomavirus Infection

Latent and Persistent Polyomavirus Infection

PCR detection of JC virus DNA in brain tissue from patients with and without progressive multifocal leukoencephalopathy

Virus-Host Interactions and Diagnosis of Human Polyomavirus-Associated Disease

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