Diagnosis of Paragonimiasis by Immunoblot

Slemenda, Susan B.; Maddison, Shirley E.; Jong, Elaine C.; Moore, Deborah

doi:10.4269/ajtmh.1988.39.469

Cited by 86 publications

(31 citation statements)

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“…These antigens are clearly different from the 8 kDa P. westermani antigen that was previously reported to be specific for paragonimiasis. 6 Another study showed that only a 31.5 kDa protein in the Paragonimus heterotremus antigen was recognized by all sera from proven paragonimiasis cases. 9 It is possible that this P. heterotremus antigen corresponds to the 34 kDa protein recognized by all NAP and P. westermani cases in this study.…”

Section: Discussionmentioning

confidence: 99%

“…In the past, worm extracts or e/s antigens from a number of old-world Paragonimus species such as P. westermani, P. heterotremus, and Paragonimus africanus have been used for serological diagnosis of paragonimiasis. 6,[15][16][17][18] Only a few studies identified single antigens of P. westermani with diagnostic potential. Since the P. kellicotti adult worm transcriptome has been sequenced (Mitreva M, McNulty SN, and others, unpublished data), we were able to compare the amino acid sequences of several of the previously described Paragonimus antigens to predicted sequences of P. kellicotti orthologues.…”

Section: Discussionmentioning

confidence: 99%

“…5 A P. westermani Chafee extract of adult worms was provided by CDC. 6,7 Excretory/secretory (e/s) antigens of P. kellicotti were obtained by culturing two adult flukes (65 days p.i.) overnight in 500 μL phosphate buffered saline (PBS) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

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Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

Fischer

Curtis²,

Folk³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. We studied the value of an IgG Western blot (WB) with Paragonimus kellicotti (Pk) antigen for diagnosis of North American paragonimiasis. The test was evaluated with sera from patients with Pk and Paragonimus westermani infections, with control sera from patients with other helminth infections, and sera from healthy Americans. All 11 proven Pk infection sera and two samples from suspected cases that were negative by P. westermani WB at the Centers for Disease Control and Prevention (CDC) contained antibodies to antigens at 34 kDa and at 21/23 kDa. Seven of 7 P. westermani sera contained antibodies to the 34 kDa antigen, but only 2 recognized the 21/23 kDa doublet. No control samples were reactive with these antigens. Antibody reactivity declined after praziquantel treatment. Thus, the P. kellicotti WB appears to be superior to P. westermani WB for diagnosing Pk infections, and it may be useful for assessing responses to treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

Fischer

Curtis²,

Folk³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…As these parasites did not exist in Ivory Coast, these cross reactions might be due to Fasciola gigantica and/or local schistosomes (S. japonicum imperfectly crossed with S. haematobium and S. mansoni). Another African paragonimid species: P. uterobilateralis, might also be the cause of these cross reactions, although Slemenda et al (1988) had reported that antibody detection for Paragonimus westermani did not allow that of other Paragonimus species. Further clinical and/or serological investigations in these 41 patients would be necessary to verify either of these both hypotheses.…”

Section: Discussionmentioning

confidence: 99%

First findings on the seroepidemiology of human paragonimosis at the anti-tuberculosis centre of Divo, Republic of Ivory Coast (West Africa)

Aka

Assoumou²,

Adoubrynk³

et al. 2008

Parasite

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Summary :An epidemiological study was carried out in [2004][2005] at the anti-tuberculosis centre of Divo (Ivory Coast) to collect sera from patients who consulted for tuberculosis suspicion and to estimate the seroprevalence of human paragonimosis in the context of a systematic screening. No Paragonimus egg was found in the stools and/or sputa of the 167 persons investigated. In contrast, 41 sera were ascertained with antibodies against Paragonimus africanus using ELISA testing. As the optical density (OD) values related to seropositive findings were found under 0.6 (the minimal OD to detect an active paragonimosis), the above antibody titres might originate from patients in chronic or in convalescent stages, or might result of cross reactions with trematodes. Concomitantly, dissection of local crabs (Callinectes marginatus) demonstrated the presence of Paragonimus metacercariae in six out of 34 examined. The parasite burdens in crabs ranged from two to 35 cysts with a mean diameter of 302 µm. In Ivory Coast, the locality of Divo must be considered an at-risk zone in reason of the presence of anti-Paragonimus antibodies in several human sera and the presence of infected crabs at the local market. Résumé

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“…Other new P. kellicotti sequences showed that P. kellicotti is more similar to P. mexicanus and several other Paragonimus species than it is to P. westermani , a species from East Asia, which is currently used at the Centers for Disease Control and Prevention in Atlanta for serologic diagnosis of paragonimiasis. 22,23 Prior studies have used molecular diagnostics to speciate Paragonimus metacerariae in snails. [24][25][26] However, relatively few studies have attempted to detect Paragonimus DNA in the definitive host.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

Fischer¹,

Curtis²,

Marcos³

et al. 2011

The American Society of Tropical Medicine and Hygiene

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Human paragonimiasis is an emerging disease in Missouri. To characterize local parasites, we examined crayfish from three rivers. Metacercaeriae consistent with Paragonimus kellicotti were detected in 69%, 67%, and 37% of crayfish from the Big Piney, Huzzah, and Black Rivers, respectively. Sequencing of the second internal transcribed spacer and other DNA markers confirmed the species identification and the presence of identical parasite sequences in clinical specimens from two human cases. Mongolian gerbils were infected by intraperitoneal injection with 3–8 metacercariae. Most gerbils died 15–49 days post-infection. Necropsies showed pulmonary hemorrhage with necrosis, and flukes as long as 8 mm were recovered from intrathoracic tissues. Western blot analysis using P. kellicotti antigen showed a strong antibody response in gerbils 39 days post-infection. These results demonstrate that P. kellicotti is common in Missouri crayfish. The gerbil model may be useful for research on the pathogenesis, immunology, and treatment of paragonimiasis.

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Diagnosis of Paragonimiasis by Immunoblot

Cited by 86 publications

References 0 publications

Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

Serological Diagnosis of North American Paragonimiasis by Western Blot Using Paragonimus kellicotti Adult Worm Antigen

First findings on the seroepidemiology of human paragonimosis at the anti-tuberculosis centre of Divo, Republic of Ivory Coast (West Africa)

Molecular Characterization of the North American Lung Fluke Paragonimus kellicotti in Missouri and its Development in Mongolian Gerbils

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