Diagnosis of ovarian tumour tissues by SR-FTIR spectroscopy: A pilot study

Grzelak, Maria Magdalena; Wróbel, Paweł; Lankosz, M.; Stęgowski, Z.; Chmura, Łukasz; Adamek, Dariusz; Hesse, Bernhard; Castillo‐Michel, Hiram

doi:10.1016/j.saa.2018.05.070

Cited by 30 publications

(19 citation statements)

References 19 publications

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“…One half of each of the root exudate dry matter collected was used for the preliminary investigation of major functional groups using Fourier Transform Infrared (FTIR) spectroscopy, which was carried out at Beamline ID21 at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France. Analysis was conducted on the Thermo Nicolet Nexus spectrometer (Artisan Technology Group, Champaign, IL, USA) associated with a SMART-orbit Attenuated Total Reflection (ATR) accessory as described previously [89]. All acquisitions were performed in the mid-IR spectral range (1700-800 cm − 1 ) in air.…”

Section: Collection and Metabolome Profiling Of Root Exudatesmentioning

confidence: 99%

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

et al. 2020

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Background: Phosphorus (P) deficiency limits crop production worldwide. Crops differ in their ability to acquire and utilise the P available. The aim of this study was to determine root traits (root exudates, root system architecture (RSA), tissue-specific allocation of P, and gene expression in roots) that (a) play a role in P-use efficiency and (b) contribute to large shoot zinc (Zn) concentration in Brassica oleracea. Results: Two B. oleracea accessions (var. sabellica C6, a kale, and var. italica F103, a broccoli) were grown in a hydroponic system or in a high-throughput-root phenotyping (HTRP) system where they received Low P (0.025 mM) or High P (0.25 mM) supply for 2 weeks. In hydroponics, root and shoot P and Zn concentrations were measured, root exudates were profiled using both Fourier-Transform-Infrared spectroscopy and gas-chromatography-mass spectrometry and previously published RNAseq data from roots was reexamined. In HTRP experiments, RSA (main and lateral root number and lateral root length) was assessed and the tissue-specific distribution of P was determined using micro-particle-induced-X-ray emission. The C6 accession had greater root and shoot biomass than the F103 accession, but the latter had a larger shoot P concentration than the C6 accession, regardless of the P supply in the hydroponic system. The F103 accession had a larger shoot Zn concentration than the C6 accession in the High P treatment. Although the F103 accession had a larger number of lateral roots, which were also longer than in the C6 accession, the C6 accession released a larger quantity and number of polar compounds than the F103 accession. A larger number of P-responsive genes were found in the Low P treatment in roots of the F103 accession than in roots of the C6 accession. Expression of genes linked with "phosphate starvation" was upregulated, while those linked with iron homeostasis were down-regulated in the Low P treatment. Conclusions: The results illustrate large within-species variability in root acclimatory responses to P supply in the composition of root exudates, RSA and gene expression, but not in P distribution in root cross sections, enabling P sufficiency in the two B. oleracea accessions studied.

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Section: Collection and Metabolome Profiling Of Root Exudatesmentioning

confidence: 99%

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

et al. 2020

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“…Ovarian cancer (OC) is the seventh most frequently occurring cancer in women and characterized by a high mortality rate of up to 40-50%, due to the fact that most OC cases are diagnosed in the late stage. 1,2 OC is xenogeneic and influenced by generated factors related to epigenetic factors, as well as several chemokines and cytokines interacting with each other to accelerate the metastasis of OC cells. 3 Most malignant ovarian tumors may originate from the surface epithelium and/or fallopian tube of the ovary, out of which more than 90% are carcinomas, with a poor prognosis and a low overall 5-year survival rate.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-23a depletion promotes apoptosis of ovarian cancer stem cell and inhibits cell migration by targeting DLG2

Zhuang

Bai

Liu

2019

Cancer Biology & Therapy

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“…The absorbance minima determined for all tissues in the protein region are vibrations of amide I (1700–1630 cm −1 ) and amide II (1580–1500 cm −1 ) functional groups 10–12 . The peaks localised at 1695 cm −1 , 1682 cm −1 and 1639 cm −1 are characteristic for aggregated β-sheet, β-turn, and β-sheet structures, respectively 11,13 .…”

Section: Resultsmentioning

confidence: 99%

“…Typically, the minimum found at 1655 cm −1 is attributed to α-helix structures of amide I 13 . The (N–H) bending coupled to (C–N) symmetric stretching vibrations assigned to amide II are localised at 1571 cm −1 and 1541 cm −1 12,14 . Of note is a peak found at 1515 cm −1 , typically attributed to (C–H) bending vibrations of tyrosine 15 .…”

Section: Resultsmentioning

confidence: 99%

Classification of aggressive and classic mantle cell lymphomas using synchrotron Fourier Transform Infrared microspectroscopy

Kołodziej

Jesionek-Kupnicka

Braun

et al. 2019

Sci Rep

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Mantle cell lymphoma (MCL) is regarded as an incurable neoplasm, even to the novel drug strategies. It is known MCL has two morphological variants- classic and aggressive. Aggressive MCL is characterized by a higher mitotic index and proliferation rate, and poor overall survival in comparison to classic subtype. The insight into the detailed biochemical composition of MCL is crucial in the further development of diagnostic and treatment guidelines for MCL patients; therefore Synchrotron radiation Fourier Transform Infrared (S-FTIR) microspectroscopy combined with Principal Component Analysis (PCA) was used. The major spectral differences were observed in proteins and nucleic acids content, revealing a classification scheme of classic and aggressive MCLs. The results obtained suggest that FTIR microspectroscopy has reflected the histopathological discrimination of both MCL subtypes.

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Diagnosis of ovarian tumour tissues by SR-FTIR spectroscopy: A pilot study

Cited by 30 publications

References 19 publications

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

Effect of phosphorus supply on root traits of two Brassica oleracea L. genotypes

MicroRNA-23a depletion promotes apoptosis of ovarian cancer stem cell and inhibits cell migration by targeting DLG2

Classification of aggressive and classic mantle cell lymphomas using synchrotron Fourier Transform Infrared microspectroscopy

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