Diagnosis of microfilaria using modified Knott’s technique in buffalo and its treatment

Abstract

Filariasis is a vector-transmitted parasitic disease caused by Filarioidea nematode with world wide spread especially in tropical and sub-tropical countries. This is highly prevalent in hot and humid areas of India mainly hilly parts of India like Tarai region of Uttar Pradesh and coastal areas of Andhra Pradesh. The tentative diagnosis was carried out by direct faecal smear examinations, wet blood film observation and blood smear examinations, and modified Knott's technique, further case history was reviewed. The collected blood was subjected to different staining techniques including modified Knott's technique. On modified Knott's method examination detected microfilaria is long slender anterior blunt and tapering posterior ends. Ivermectin 200 μg/kg body weight subcutaneous route (orally microfilaricide 50 ug/ kg) is used as a curative drug in present clinical case and animal was responded well with the treatment. Hematological parameters of buffalo includes decreased hemoglobin (6.5 grs /dl), packed cell volume (20%), and total erythrocytic count (4.5×106/ul) were recovered within 1 week after treatment.

Morphology of Setaria spp. (Setariidae; Nematoda) in Aceh cattle, Indonesia

“…The examination result of 150 blood samples from Aceh cattle to determine the presence of Setaria spp. microfilaria by using Modified Knott's Technique (Weerasekara et al 2019;Basude 2023) appeared in Table 2, whereas the blood examination of Aceh cattle by hematocrit microcapillary method showed in Table 3 and Figures 3A and 3B. The microfilaria infection rate of Setaria spp.…”

“…Observation of Setaria spp. microfilaria in blood was conducted by Modified Knott's Technique method (Weerasekara et al 2019;Basude 2023). The blood samples were collected and put inside Ethylene Diamine Tetraacetic acid (EDTA) mixture.…”