Diagnosis of larval cyathostominosis in horses in Belgium

Smets, Katelijne; Shaw, Darren; Deprez, Piet; Vercruysse, Jozef

doi:10.1136/vr.144.24.665

Cited by 23 publications

(29 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…25% of cases in our group (5, 8 and 9) were presented because of rapid weight loss, subcutaneous oedemas and mild intermittent diarrhoea in some of them. Similarly in the studies of Smets et al (1999) and Peregrine et al (2006) reporting 24 cases, weight loss and colic were the second most common clinical signs of cyathostomosis after diarrhoea. In case 5 of our group homeostasis was severely affected despite the fact that the horse did not exhibit diarrhoea.…”

Section: Discussionmentioning

confidence: 58%

See 1 more Smart Citation

Equine cyathostomosis: case reports

Bodeček¹,

Jahn²,

Dobešová³

et al. 2010

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:Twelve clinical cases of cyathostomosis in horses treated at the Equine Clinic University of Veterinary and Pharmaceutical Sciences in Brno, the Czech Republic, between the years 1999 and 2008 are described in this report. Six cases (50%) were hospitalized in the period from 2007 to 2008. Eleven of them were hospitalized in the period from December to March. Only one case was admitted in June, but the clinical signs had appeared for the first time in January. All horses described in these cases were younger than six years of age. Diarrhoea as a predominant clinical sign was present in four horses and colic in four horses. One horse showed both colic and diarrhoea whilst three horses had weight loss and subcutaneous oedema. Metabolic acidosis was found in three horses, eight patients had leucocytosis. Hypoproteinemia was found in four horses, hypoalbuminemia in seven horses, hypokalemia in three horses and increased alkaline phosphatase (ALP) activity in five horses. Seven horses recovered, one horse died and four horses were euthanized.

show abstract

Section: Discussionmentioning

confidence: 58%

“…Clinical signs include diarrhoea (Mair et al, 1990;Love et al, 1992;Mair, 1993), subcutaneous oedema, intermittent colics and weight loss Smets et al, 1999). Several different clinical syndromes connected with cyathostome infection are distinguished by Mair (2002): 1.…”

mentioning

confidence: 99%

Equine cyathostomosis: case reports

Bodeček¹,

Jahn²,

Dobešová³

et al. 2010

Vet. Med. - Czech

View full text Add to dashboard Cite

show abstract

“…Clinical diagnosis of this disease can be difficult and relies on various parameters (Giles et al, 1985;Reilly et al, 1993;Mair, 1994;Smets et al, 1999). Clinicians have previously ascribed variation in clinical signs to the presence of different cyathostomin species, level of larval burden or individual host immune response (Mair, 1994).…”

Section: Discussionmentioning

confidence: 99%

A PCR–ELISA for the identification of cyathostomin fourth-stage larvae from clinical cases of larval cyathostominosis

Hodgkinson

Lichtenfels

Mair

et al. 2003

International Journal for Parasitology

View full text Add to dashboard Cite

We report the use of six oligoprobes designed from intergenic spacer region sequences to identify fourth-stage larvae (L4) of the tribe Cyathostominae. Oligoprobes were designed for identification of the following species: Cylicocyclus ashworthi, Cylicocyclus nassatus, Cylicocyclus insigne, Cyathostomum catinatum, Cylicostephanus goldi, and Cylicostephanus longibursatus. A seventh probe was designed as a positive control to identify all these members of the Cyathostominae. The intergenic spacer region was amplified by PCR using conserved primers. Initially, three oligoprobes were used in Southern blot analysis. To facilitate high-throughput identification, these and a further four oligoprobes were developed for use in a PCR -ELISA. All probes were validated for their ability to detect cyathostomin PCR products in the PCR -ELISA, using DNA from morphologically identified adult parasites. Initially, 712 L4 were isolated from the diarrhoeic faeces from horses (n ¼ 17) with clinical larval cyathostominosis. PCR products from 522 of these L4 were subjected to analysis, with 413 L4 being identified as one of the aforementioned species. With reference to individual species analysis, 28.5% of the 522 L4 were identified as C. longibursatus, 25.7% as C. nassatus, 15.9% as C. ashworthi, 7.3% as C. goldi and 1.7% as C. catinatum. No L4 were identified as being C. insigne species. When L4 within faeces from individual horses were compared, no sample was found to comprise parasites of one species. The least number of species identified in a single sample was two. This study suggests that clinical larval cyathostominosis is predominantly caused by mixed-species infections. q

show abstract

“…Diagnosis of cyathostomiasis is challenging and definite confirmation can so far only be obtained by post mortem examination or, in selected cases, by examination of large intestinal or rectal biopsies (Love 1992, Peregrine et al 2006. Faecal examination may reveal L4 larvae and adult parasites in 30% or strongyle eggs in 36% of horses with clinical signs compatible with cyathostomiasis, respectively (Smets et al 1999). Treatment includes larvicidal doses of fenbendazole (10mg/kg for 5 consecutive days) (Lyons et al 2000).…”

Section: Other Parasitesmentioning

confidence: 99%

Diagnosis and treatment of non-infectious and infectious foal diarrhoea

Dunkel¹

2008

PHK

View full text Add to dashboard Cite

Diagnosis of larval cyathostominosis in horses in Belgium

Cited by 23 publications

References 9 publications

Equine cyathostomosis: case reports

Equine cyathostomosis: case reports

A PCR–ELISA for the identification of cyathostomin fourth-stage larvae from clinical cases of larval cyathostominosis

Diagnosis and treatment of non-infectious and infectious foal diarrhoea

Contact Info

Product

Resources

About