Diagnosis of Intestinal Amebiasis Using Salivary IgA Antibody Detection

Muro, R del; Acosta, E.; Merino, Emilio F.; Glender, Walter; Ortiz‐Ortiz, Librado

doi:10.1093/infdis/162.6.1360

Cited by 28 publications

(10 citation statements)

References 23 publications

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“…In addition, salivary antibodies concentration also presents diagnostic value for intestinal infection due to parasitic agents such as Entamoeba histolytica [133, 134], Trichuris trichiura , Ascaris lumbricoides , and hookworms [135]. Another prominent infectious parasitic organism is Toxoplasma gondii that causes toxoplasmosis disease.…”

Section: Salivary Antibodiesmentioning

confidence: 99%

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Malon

Sadir

Malarvili

et al. 2014

BioMed Research International

183

108

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Saliva is increasingly recognised as an attractive diagnostic fluid. The presence of various disease signalling salivary biomarkers that accurately reflect normal and disease states in humans and the sampling benefits compared to blood sampling are some of the reasons for this recognition. This explains the burgeoning research field in assay developments and technological advancements for the detection of various salivary biomarkers to improve clinical diagnosis, management, and treatment. This paper reviews the significance of salivary biomarkers for clinical diagnosis and therapeutic applications, with focus on the technologies and biosensing platforms that have been reported for screening these biomarkers.

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Section: Salivary Antibodiesmentioning

confidence: 99%

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Malon

Sadir

Malarvili

et al. 2014

BioMed Research International

183

108

View full text Add to dashboard Cite

show abstract

“…The anti‐amoebic sIgA antibodies can be detected in secretions of several compartments belonging to the GALT such as ileum, colon and faeces of patients with ALA as well as calostrum, milk and saliva from patients with amoebic dysentery. Moreover, the search of those antibodies in the different body fluids has been intended as a diagnostic tool of intestinal amoebiasis (30).…”

Section: Secretory Immune Response In the Infection By Entamoeba Histmentioning

confidence: 99%

“…Initial studies suggested that the sIgA response raised during the intestinal infection with E. histolytica was short‐lasting, remaining just for few weeks. However, recent studies have demonstrated that this response is long‐lasting, up to 1 year, and noteworthy, its persistence could protects against intestinal re‐infection with E. histolytica in patients of endemic areas (28–31). Moreover, sIgA response, particularly evaluated against the Gal‐lectin, was also sustained by over 18 months in patients cured of ALA and it was associated to immunity against the nonpathogenic E. dispar ; however, immunity against E. histolytica could not be determined in that study (32).…”

Section: Secretory Immune Response In the Infection By Entamoeba Histmentioning

confidence: 99%

The role of the secretory immune response in the infection by Entamoeba histolytica

Carrero

Cervantes-Rebolledo

Aguilar-Díaz

et al. 2007

Parasite Immunology

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Intestinal infection with the protozoan parasite Entamoeba histolytica elicits a local immune response with rising of specific secretory IgA (sIgA) antibodies detectable in several compartments associated to mucosa. Anti-amoebic sIgA antibodies have been reported in faeces, saliva, bile and breast milk from dysenteric patients and research trying to elucidate their role in protection has recently intensified. IgA antibodies inhibit the in vitro adherence of E. histolytica trophozoites to epithelial cell monolayers by recognizing several membrane antigens, including the galactose-binding lectin (Gal-lectin), main surface molecule involved in adherence, and the serine and cystein-rich proteins, all of them potential vaccine candidates. In fact, the presence of sIgA anti-Gal lectin in faeces of patients recovered from amoebic liver abscess (ALA) was associated with immunity to E. dispar. Moreover, the combined nasal and intraperitoneal vaccination of C3H/HeJ mice with native and recombinant Gal-lectin protected mice against an intracecal challenge with virulent E. histolytica trophozoites, protection that seemed to be associated with the induction of specific intestinal sIgA antibodies. Therefore, the stimulation of intestinal secretory response by mucosal delivery of amoebic antigens has been positioned as a promising strategy for inducing protection against human amoebiasis.

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“…Anti-lectin IgA is a well-defined surrogate marker for immunity to amebiasis, and the Gal/GalNAc lectin is a promising candidate for vaccine development. Anti-lectin IgA was identified in saliva of school children with intestinal infection of E. histolytica (del Muro et al, 1990). It was also found in patients who developed invasive diseases (Aceti et al, 1991;Kelsall et al, 1994).…”

Section: Acquired Immune Response: Intestinal Anti-lectin Igamentioning

confidence: 90%

Molecular biology research to benefit patients with Entamoeba histolytica infection

Watanabe

Petri

2015

Molecular Microbiology

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SummaryThe development of molecular microbiology has made it possible for us to deepen our understanding of the pathogenesis of amebiasis. Research using the trophozoite form of Entamoeba histolytica has clearly shown us the importance of the interface between the parasite and host cells in vitro. Immunopathogenesis after excystation was similarly well advanced by the use of a novel murine model of amebic colitis. However, it is still challenging to apply these findings to clinical and epidemiological settings. This is mainly because of the lack of a complete infection animal model of amebiasis by oralfecal infection. Moreover, in vitro experiments have predominantly been performed using the same axenic cultured strain HM-1: IMSS isolated about 50 years ago, whereas highly diverse strains are prevalent all over the world. Translational research informed by clinical observations has the greatest potential for the development of effective interventions. Here, we highlight discoveries of the experiments designed from cohort observation and discuss remaining problems to be solved.

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Diagnosis of Intestinal Amebiasis Using Salivary IgA Antibody Detection

Cited by 28 publications

References 23 publications

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

Saliva-Based Biosensors: Noninvasive Monitoring Tool for Clinical Diagnostics

The role of the secretory immune response in the infection by Entamoeba histolytica

Molecular biology research to benefit patients with Entamoeba histolytica infection

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