Diagnosis of human granulocytic anaplasmosis in Belgium by combining molecular and serological methods

Hing, Mony; Woestyn, Sophie; Bosterhaut, B. Van; Desbonnet, Y.; Heyman, Paul; Cochez, Christel; Silaghi, Cornelia; Sprong, Hein; Fournier, Pierre‐Edouard; Raoult, Didier; Neirinckx, Pierre; Heuninckx, W.

doi:10.1002/nmi2.65

Cited by 14 publications

(3 citation statements)

References 12 publications

(12 reference statements)

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“…Only a few studies have investigated the incidence of other TBDs transmitted by I. ricinus , such as anaplasmosis and babesiosis, in Europe [ 75 ]. For example, a sero-epidemiological study estimated between 10 and 40 human anaplasmosis cases in Belgium per year [ 11 , 76 ]. Human cases of other TBDs are being reported in the literature, mostly as case studies or series.…”

Section: Policy Driven Changes In Abundance and Spreadmentioning

confidence: 99%

Control of Lyme borreliosis and other Ixodes ricinus-borne diseases

et al. 2018

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Lyme borreliosis (LB) and other Ixodes ricinus-borne diseases (TBDs) are diseases that emerge from interactions of humans and domestic animals with infected ticks in nature. Nature, environmental and health policies at (inter)national and local levels affect the risk, disease burden and costs of TBDs. Knowledge on ticks, their pathogens and the diseases they cause have been increasing, and resulted in the discovery of a diversity of control options, which often are not highly effective on their own. Control strategies involving concerted actions from human and animal health sectors as well as from nature managers have not been formulated, let alone implemented. Control of TBDs asks for a “health in all policies” approach, both at the (inter)national level, but also at local levels. For example, wildlife protection and creating urban green spaces are important for animal and human well-being, but may increase the risk of TBDs. In contrast, culling or fencing out deer decreases the risk for TBDs under specific conditions, but may have adverse effects on biodiversity or may be societally unacceptable. Therefore, in the end, nature and health workers together must carry out tailor-made control options for the control of TBDs for humans and animals, with minimal effects on the environment. In that regard, multidisciplinary approaches in environmental, but also medical settings are needed. To facilitate this, communication and collaboration between experts from different fields, which may include patient representatives, should be promoted.

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Section: Policy Driven Changes In Abundance and Spreadmentioning

confidence: 99%

Control of Lyme borreliosis and other Ixodes ricinus-borne diseases

et al. 2018

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“…Both experimental and field studies have pointed out the utility of molecular laboratory diagnosis for sensitive and specific identification of Anaplasma infections (Eriks et al 1989, Palmer et al 1998, Eddlestone et al 2007, Haigh et al 2008, Hing et al 2014. The growing number of highperformance molecular protocols with increased potential for automation and multiplex detection has resulted in these becoming indispensable laboratory tools.…”

Section: Molecular Detectionmentioning

confidence: 99%

European Network for Neglected Vectors and Vector-Borne Infections COST Action Guidelines: What Is This About and What Is This For?

Charrel

Lempereur

Mihalca

et al. 2017

Vector-Borne and Zoonotic Diseases

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“…In a clinical setting, real-time polymerase chain reaction (qPCR) is widely used as a time-and cost-effective specific method for TBP detection during the acute phase of infection [4,21]. As the infectious dose for TBPs may be low, a low detection limit is required to detect the pathogen, especially in the early stage of the disease [22].…”

Section: Introductionmentioning

confidence: 99%

Technical Evaluation of qPCR Multiplex Assays for the Detection of Ixodes ricinus-Borne Pathogens

et al. 2022

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Background: The extent to which infections with Ixodes ricinus-borne pathogens (TBPs), other than Borrelia burgdorferi s. l. and tick-borne encephalitis virus (TBEV), cause disease in humans remains unclear. One of the reasons is that adequate diagnostic modalities are lacking in routine or research settings. Methods: We evaluated the analytical specificity, sensitivity and robustness of qPCR assays for the detection of Anaplasma phagocytophilum, Neoehrlichia mikurensis, Spiroplasma ixodetis, several Babesia species and Spotted Fever Rickettsia species as well as Bartonella species in human samples. Results: The qPCRs were found to perform well, given the difficulties of dealing with microorganisms for which confirmed patient materials are scarce or non-existent, a hurdle that was partially overcome by using synthetic controls. Spiking blood samples with the tested microorganisms showed that the detection of the TBPs was not inhibited by the presence of blood. The acceptable sensitivity when multiplexing the different pathogens, the good inter-assay variability and the absence of cross-reactivity make them potentially suitable as human diagnostics. Conclusions: The qPCRs evaluated in this study are technically suitable for the laboratory diagnostic assessment of clinical samples for infection with tick-borne pathogens. However, clinical validation and independent confirmation are still needed, pending the availability of sufficient human samples for testing in different laboratories.

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Diagnosis of human granulocytic anaplasmosis in Belgium by combining molecular and serological methods

Abstract: We report here one new, hospitalized case of Anaplasma phagocytophilum in Belgium. The clinical presentation of anaplasmosis, its treatment and the molecular and serological relevant laboratory methods are briefly developed.

Cited by 14 publications

References 12 publications

Control of Lyme borreliosis and other Ixodes ricinus-borne diseases

Control of Lyme borreliosis and other Ixodes ricinus-borne diseases

European Network for Neglected Vectors and Vector-Borne Infections COST Action Guidelines: What Is This About and What Is This For?

Technical Evaluation of qPCR Multiplex Assays for the Detection of Ixodes ricinus-Borne Pathogens

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