Diagnosis of HIV infection and laboratory monitoring of its therapy

Yılmaz, Gülden

doi:10.1016/s1386-6532(01)00165-2

Cited by 24 publications

(9 citation statements)

References 34 publications

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“…This increased variability appeared in run 5, which contributed to the increased intervari- VOL. 47,2009 EVALUATING THE Abbott m2000 RealTime HIV-1 ASSAY 2211…”

Section: Resultsmentioning

confidence: 99%

“…In September 2007, approximately 329,000 individuals received antiretroviral (ARV) treatment as part of the national program (24a, 40). Globally, viral load (VL) testing has been used to monitor treatment, determine the prognosis and the risk of disease progression, and identify treatment failures (47). South African treatment guidelines use VL results to determine the time for the switch from a first-line to a second-line drug regimen (24).…”

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confidence: 99%

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Evaluation of the Abbott m 2000 RealTi m e Human Immunodeficiency Virus Type 1 (HIV-1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep-Cobas Amplicor, Roche Cobas AmpliPrep-Cobas TaqMan HIV-1, and BioMerieux NucliSENS EasyQ HIV-1 Assays

et al. 2009

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The implementation of antiretroviral therapy demands the need for increased access to viral load (VL) monitoring. Newer real-time VL testing technologies are faster and have larger dynamic ranges and fully automated extraction to benefit higher throughputs in resource-poor environments. The Abbott RealTime human immunodeficiency virus type 1 (HIV-1) assay was evaluated as a new option for testing for HIV-1 subtype C in South Africa, and its performance was compared to the performance of existing assays (the Cobas AmpliPrep-Cobas TaqMan HIV-1, version 1, assay; the AmpliPrep-Cobas Monitor standard HIV-1 assay; and the NucliSENS EasyQ-EasyMag HIV-1 assay) in a high-throughput laboratory. The total precision of the RealTime HIV-1 assay was acceptable over all viral load ranges. This assay compared most favorably with the Cobas AmpliPrep-Cobas TaqMan HIV-1 assay (R 2 ‫؍‬ 0.904), with a low standard deviation of difference being detected (0.323 copies/ml). The bias against comparator assays ranged from ؊0.001 copies/ml to ؊0.228 copies/ml. Variability in the reporting of VLs for a 20-member subtype panel compared to the variability of other assays was noted with subtypes G and CRF02-AG. The RealTime HIV-1 assay can test 93 samples per day with minimal manual preparation, less staff, and the minimization of contamination through automation. This assay is suitable for HIV-1 subtype C VL quantification in South Africa.

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“…This increased variability appeared in run 5, which contributed to the increased intervari- VOL. 47,2009 EVALUATING THE Abbott m2000 RealTime HIV-1 ASSAY 2211…”

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Evaluation of the Abbott m 2000 RealTi m e Human Immunodeficiency Virus Type 1 (HIV-1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep-Cobas Amplicor, Roche Cobas AmpliPrep-Cobas TaqMan HIV-1, and BioMerieux NucliSENS EasyQ HIV-1 Assays

et al. 2009

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“…The qualitative and quantitative evaluation of both serum HIV-1 RNA genome and HIV-1 proviral DNA are pivotal markers in the diagnosis and prognosis of HIV-1 infection (7, 8). Although the quantitative determination of plasma HIV RNA copies directly represents viral replication and is the main prognostic parameter for disease progression (9), the HIV-1 proviral DNA load represents the infection reservoir in PBMC and lymphoid tissues and plays a pivotal role in immune surveillance escape (2, 10).…”

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confidence: 99%

Factors Associated with HIV-1 Proviral DNA Loads in Patients with Undetectable Plasma RNA Load

et al. 2009

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To evaluate factors associated with human immunodeficiency virus type 1 (HIV-1) proviral DNA load, we conducted a cross-sectional study of 36 chronically HIV-1-infected individuals with undetectable plasma viral RNA. We used real-time polymerase chain reaction to determine the number of HIV-1 proviral DNA copies per 106 peripheral blood mononuclear cells. The mean level of plasma viral RNA when the CD4+ T cell count was above 500 cells/µL without highly active antiretroviral therapy (HAART) was significantly associated with proviral DNA load at the time of undetectable plasma HIV RNA with HAART. Strategies to reduce the level of plasma viral RNA when patients' CD4+ T cell counts are above 500 cells/µL without HAART could help reduce HIV-1 proviral DNA load.

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“…El HIV Ag/Ac está diseñado para detectar antígeno p24 así como anticuerpos contra HIV-1, HIV-1 grupo O y HIV-2 (28) . Diversas evaluaciones entre estos métodos demuestran ventas y desventajas símiles, siendo importante su valoración concernientes a interferentes, rendimiento diagnóstico y costo-efectividad (29,30,31) . La variación en el rendimiento de cada método puede deberse al tamaño de análisis y al flujo de pacientes seropositivos durante este tiempo dado que se utilizaron por separado (Tabla 1).…”

Section: Discussionunclassified

Efecto del extracto acuoso, ácido y alcohólico de las hojas secas de Erythroxylum coca var coca (coca) en Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Candida albicans in vitro

Moya‐Salazar

Pio-Dávila

2017

HorizMed

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RESUMENObjetivo: Evaluar la depleción del suministro de sangre y el costo por donaciones indeterminadas del Hospital Nacional Guillermo Almenara Irigoyen durante el 2014. Materiales y métodos: Estudio retrospectivo de corte transversal y análisis de costo-utilidad en donaciones con resultados en zona gris (valor de la muestra/ valor de corte entre los límites > 0.85 y < 1) luego del tamizaje serológico para los siete marcadores infecciosos (HIV, HBsAg, HBcAb, HCV, HTLV-1/2, SÍFILIS y CHAGAS) y la determinación en simultáneo del HIV Ag/Ab combo, por ELISA de cuarta generación. Desde el sistema e-Delphyn® se codificaron y tabularon los datos. El análisis de costos se realizó considerando el tipo de cambio vigente. Resultados: De 9 560 donaciones, el 20.7 % (1977 donaciones) fueron resultados en zona gris lo que ocasionó una pérdida de 863.9 litros de sangre y 92 640 USD. HbcAb con 10.18% (973 resultados indeterminados) y anti-HIV con 0.39% (37 resultados indeterminados) fueron los resultados con mayor y menor seroprevalencia, respectivamente (p<0.05). No se encontró diferencias significativas entre los marcadores anti-HIV y HIV Ag/Ab combo (p=0.776). Conclusiones: Se demostró que >800 litros de sangre descartada generó >90 mil USD de costo por donaciones indeterminadas ocasionando un perjuicio económico al presupuesto hospitalario y una disminución del suministro de sangre disponible para el tratamiento de pacientes en el Hospital Nacional Guillermo Almenara Irigoyen.Palabras clave: Zona gris; donación de sangre; marcadores infecciosos; tamizaje. Depletion of blood supply and cost due to indeterminate donations at the Guillermo Almenara Irigoyen National Hospital ABSTRACTObjective: To evaluate the depletion of blood supply and the cost due to indeterminate donations at Hospital Nacional Guillermo Almenara Irigoyen during 2014. Materials and methods: A retrospective cross-sectional study and a cost-utility analysis were conducted in donations showing results in the gray zone (sample value/cutoff value between >0.85 and <1) after a serological screening of seven infectious markers (HIV, HBsAg, HBcAb, HCV, HTLV-1/2, syphilis and Chagas disease) and the simultaneous determination of the HIV Ag/Ab combo by means of a fourth generation ELISA. Data was encoded and tabulated using the e-Delphyn® system. The cost-utility analysis was performed considering the current exchange rate. Results: Out of 9,560 donations, 20.7% (1977 donations) showed results in the gray zone which caused a loss of 863.9 liters of blood and USD 92,640. The highest and lowest rate of seroprevalence were observed in HBcAb with 10.18% (973 indeterminate test results) and anti-HIV with 0.39% (47 indeterminate test results), respectively (p<0.05). No significant differences were found between the anti-HIV y HIV Ag/Ab combo screening methods (p=0.776). Conclusions: It was demonstrated that more than 800 liters of discarded blood generated a more than USD 90,000 cost due to indeterminate donations causing economic damages to the hospital budget and a de...

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Diagnosis of HIV infection and laboratory monitoring of its therapy

Cited by 24 publications

References 34 publications

Evaluation of the Abbott m 2000 RealTi m e Human Immunodeficiency Virus Type 1 (HIV-1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep-Cobas Amplicor, Roche Cobas AmpliPrep-Cobas TaqMan HIV-1, and BioMerieux NucliSENS EasyQ HIV-1 Assays

Evaluation of the Abbott m 2000 RealTi m e Human Immunodeficiency Virus Type 1 (HIV-1) Assay for HIV Load Monitoring in South Africa Compared to the Roche Cobas AmpliPrep-Cobas Amplicor, Roche Cobas AmpliPrep-Cobas TaqMan HIV-1, and BioMerieux NucliSENS EasyQ HIV-1 Assays

Factors Associated with HIV-1 Proviral DNA Loads in Patients with Undetectable Plasma RNA Load

Efecto del extracto acuoso, ácido y alcohólico de las hojas secas de Erythroxylum coca var coca (coca) en Trichophyton rubrum, Trichophyton mentagrophytes, Microsporum canis y Candida albicans in vitro

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