Diagnosis of histoplasmosis by detection of the internal transcribed spacer region of fungal rRNA gene from a paraffin-embedded skin sample from a dog in Japan

Ueda, Yachiyo; Sano, Ayako; Tamura, Miki; Inomata, Tomo; Kamei, Katsuhiko; Yokoyama, Koji; Kishi, Fukuko; Itô, Junko; Mikami, Yuzuru; Miyaji, Makoto; Nishimura, Kazuko

doi:10.1016/s0378-1135(03)00104-4

Cited by 34 publications

(25 citation statements)

References 7 publications

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“…farciminosum in the USA (AB071838) indicated that autochthonous human cases have been caused by the same genotype of H. capsulatum var. farciminosum as that found in dogs [8].…”

Section: Introductionsupporting

confidence: 72%

Molecular epidemiology of canine histoplasmosis in Japan

Murata¹,

Sano²,

Ueda³

et al. 2007

Med Mycol

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A recent case of canine histoplasmosis, the first confirmed case of disseminated infection accompanied by carcinoma in Japan, was diagnosed by clinical characteristics, histopathological examination, chest radiographs, ocular fundoscopy and molecular biological data. The clinical manifestations were not limited to cutaneous symptoms but were referable to disseminated infection, similar to human autochthonous cases. The partial sequences of the internal transcribed spacer (ITS1/2) regions of the ribosomal DNA genes of this and other Japanese canine histoplasmosis strains were 99-100% identical to the sequence AB211551 derived from a human isolate in Thailand, and showed a close relationship to the sequences derived from Japanese autochthonous systemic and cutaneous human cases. The phylogenetic analysis of 97 sequences of the ITS1/2 region disclosed six genotypes. The genotypes derived from Japanese autochthonous human and dog cases belonged to the cluster consisting of Histoplasma capsulatum var. capsulatum and H. capsulatum var. farciminosum sequences, indicating that these varieties might cause not only cutaneous but also systemic histoplasmosis, regardless of their host species. The current status of the 3 varieties of Histoplasma capsulatum according to the host species remains a subject of further investigation.

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“…farciminosum in the USA (AB071838) indicated that autochthonous human cases have been caused by the same genotype of H. capsulatum var. farciminosum as that found in dogs [8].…”

Section: Introductionsupporting

confidence: 72%

Molecular epidemiology of canine histoplasmosis in Japan

Murata¹,

Sano²,

Ueda³

et al. 2007

Med Mycol

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“…Recently, however, autochthonous cases have been reported in humans [13]. Seven cases of canine histoplasmosis have been reported in Japan [12,17,18,20,22]. In addition, Murata et al have compared canine cases with worldwide human and equine cases with respect to partial sequences on the internal transcribed spacer (ITS1/ 2) regions of the ribosomal DNA genes [17].…”

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confidence: 99%

First Case Report of Histoplasmosis in a Cat in Japan

Kobayashi

Tanaka²,

Asai³

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Cytological, histopathological and immunohistochemical examinations were carried out on a presumed 10-year-old Japanese cat showing vomiting and emaciation. On cytologic examination of the mass of the upper abdominal cavity, many yeast-like organisms were detected in the macrophages. At necropsy, the upper part of colon was markedly dilated with a thickened wall. The lung did not show significant changes. Histologically, severe necrotic and granulomatous lesions were observed in the colon. In the colonic lesion, the cytoplasm of the macrophages contained yeast-like organisms with irregularly shaped dots, and the cell walls of these organisms were stained black by Grocott-Gomori methenamine-silver stain. Immunohistochemically, they were found to be positive for anti-histoplasma yeast antibody. This is the first report of feline histoplasmosis in Japan.

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“…Some of these markers present different types of limitations associated with complicated methodologies that involve high costs. A small number of markers have been obtained from ribosomal genes, whose conserved nature within the fungi kingdom can lead to nonspecific results among various fungal species (16,25,26,33,38,43,49). Commercially available probes used for diagnostic tests are not a panacea, because such probes have shown nonspecific results (6).…”

Section: Discussionmentioning

confidence: 99%

“…Although several immunological and molecular methods for identifying H. capsulatum have been reported (4,5,16,19,22,26,27,30,33,38,40,42,43,49), some of them have distinct limitations, such as low sensitivity and specificity (12). Among the molecular methods used for diagnosis, some fail to detect the fungal presence due to the high genetic variability of H. capsulatum.…”

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confidence: 99%