Diagnosis of grapevine esca disease by immunological detection of Phaeomoniella chlamydospora

Fleurat‐Lessard, Pierrette; Luini, Estelle; Berjeaud, Jean‐Marc; Roblin, Gabriel

doi:10.1111/j.1755-0238.2010.00106.x

Cited by 26 publications

(19 citation statements)

References 24 publications

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“…Although results of many research studies have led to esca being defined as a complex of diseases (esca disease complex), the term ‘esca’ is still commonly used to refer to most of the diseases forming the complex. The characterization of grapevine trunk diseases is crucial, not only for studying their phytotoxic properties, but also because their detection in grapevines represents a useful tool for an early diagnosis of trunk diseases (Fleurat‐Lessard et al. , 2010).…”

Section: Introductionmentioning

confidence: 99%

Grapevine trunk diseases: complex and still poorly understood

et al. 2012

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This review presents an overview of eutypa dieback, esca and botryosphaeria dieback, the predominant grapevine trunk diseases worldwide. It covers their symptomatologies in the trunk, leaves and berries; the characteristics of the different fungal species associated with them; and host-pathogen interactions. Here, the host-pathogen relationship is defined at the cytological, physiological and molecular levels. Currently available experimental tools for studying these diseases, both in vitro and in the field, are discussed. Finally, a progress report on their control, which, since the ban of sodium arsenite, comprises chemical, biological and ⁄ or sanitation methods, is presented.

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Section: Introductionmentioning

confidence: 99%

Grapevine trunk diseases: complex and still poorly understood

et al. 2012

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“…apoplexy) that is favored during hot and dry summers, and the chronicle form characterized by the expression of progressive symptoms on leaves and berries, that can be intermittent from year to year ( Mugnai et al, 1999 ; Guerin-Dubrana et al, 2012 ; Bertsch et al, 2013 ). P. chlamydospora possess all the traits of a vascular wilt pathogen including a systemic host colonization, spatial restriction to xylem vessels with limited ability to degrade structural plant cell wall polymers, and production of phytotoxins ( Abou-Mansour et al, 2004 ; Bruno and Sparapano, 2006a , b , c ; Santos et al, 2006 ; Valtaud et al, 2009 ; Fleurat-Lessard et al, 2010 ; Luini et al, 2010 ; Morales-Cruz et al, 2015 ). Several infection routes have been identified including infected nursery plant material ( Whiteman et al, 2007 ), soilborne ( Agusti-Brisach et al, 2013 ; Pouzoulet et al, 2013b ) and airborne infections ( Eskalen et al, 2007 ; Moyo et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Xylem Vessel Diameter Affects the Compartmentalization of the Vascular Pathogen Phaeomoniella chlamydospora in Grapevine

et al. 2017

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Fungal wilt diseases are a threat to global food safety. Previous studies in perennial crops showed that xylem vessel diameter affects disease susceptibility. We tested the hypothesis that xylem vessel diameter impacts occlusion processes and pathogen compartmentalization in Vitis vinifera L. We studied the interaction between four grape commercial cultivars with the vascular wilt pathogen Phaeomoniella chlamydospora. We used qPCR and wood necrotic lesion length to measure fungal colonization coupled with histological studies to assess differences in xylem morphology, pathogen compartmentalization, and fungal colonization strategy. We provided evidence that grape cultivar with wide xylem vessel diameter showed increased susceptibility to P. chlamydospora. The host response to pathogen included vessel occlusion with tyloses and gels, deposition of non-structural phenolic compounds and suberin in vessel walls and depletion of starch in parenchyma cells. Pathogen compartmentalization was less efficient in wide xylem vessels than in narrow diameter vessels. Large vessels displayed higher number of tyloses and gel pockets, which provided substrate for P. chlamydospora growth and routes to escape occluded vessels. We discuss in which capacity xylem vessel diameter is a key determinant of the compartmentalization process and in turn grape cultivar resistance to disease caused by P. chlamydospora.

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“…Study of these polypeptides could be utilized to develop a rapid and early detection method for fungal infection based on immunological assay. Polyclonal antibodies have been used previously to detect multiple isolates (Biazon et al, 2006;Fleurat-Lessard, Luini, Berjeaud, & Roblin, 2010;Gautam, Cahill, & Hardham, 1999). The present study was designed (i) to develop the polyclonal antibodies against the antigens isolated from P. aphanidermatum and F. oxysporum at different stages of disease development and (ii) to investigate the specificity and sensitivity of the developed antibodies and to determine the feasibility of ELISA test for rapid detection of the pathogens using the developed antibodies at different stages of infection.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

Ray

Dash

Achary

et al. 2017

Food and Agricultural Immunology

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Polyclonal antibodies against Pythium aphanidermatum and Fusarium oxysporum proteins were developed for the detection of rhizome rot in ginger using serological assays. Under optimal experimental conditions, the detection limit of P. aphanidermatum by indirect ELISA was 10 µg/ml with a linear working range from 5 to 100 µg/ml (R 2 = 0.994). In case of F. oxysporum, the linear working range was 5-100 µg/ml (R 2 = 0.991) and the limit of detection was 25 µg/ml. The developed antibodies showed the highest titer in ELISA at 1:2000 dilutions. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis identified proteins ranging from molecular weights 15-97 kDa and 14-116 kDa of P. aphanidermatum and F. oxysporum isolates, respectively. In Western blot analysis, the developed antisera gave positive reactions against the isolated antigens of the fungi. The antibodies revealed immune-reactive bands of molecular weights 59 and 65 kDa in P. aphanidermatum and 44 and 75 kDa in F. oxysporum. The results suggest that the developed antibodies could be successfully applied for the specific immunodetection of P. aphanidermatum and F. oxysporum at an early stage of rhizome rot disease. ARTICLE HISTORY

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Diagnosis of grapevine esca disease by immunological detection of Phaeomoniella chlamydospora

Cited by 26 publications

References 24 publications

Grapevine trunk diseases: complex and still poorly understood

Grapevine trunk diseases: complex and still poorly understood

Xylem Vessel Diameter Affects the Compartmentalization of the Vascular Pathogen Phaeomoniella chlamydospora in Grapevine

Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger

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