Diagnosis of Eimeria species using traditional and molecular methods in field studies

Carvalho, Fábio Santos; Wenceslau, Amauri Arias; Teixeira, Marcel; Carneiro, João Alexandre Matos; Melo, Antônio Diego Brandão; Albuquerque, George Rêgo

doi:10.1016/j.vetpar.2010.11.015

Cited by 61 publications

(53 citation statements)

References 22 publications

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“…After arrival, all samples were stored at 4°C until analysis. Each faecal sample was homogenised in 500 ml tap water, and then 2 g of the mixture was put into 60 ml of saturated salt solution (Carvalho et al 2011;Mundt et al 2005;Velkers, et al 2010). The suspension was then emptied into a modified McMaster chamber (Coudert et al 1995).…”

Section: Sampling and Treatment Methodsmentioning

confidence: 99%

Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China

et al. 2011

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The current study examined the prevalence of Eimeria infections in domestic rabbits in China. A total of 480 faecal samples were collected from 48 farms in 14 provinces of China. Each faecal sample was subjected to oocyst counting and oocyst isolation. The Eimeria species from samples containing isolated and sporulated oocysts were morphologically identified under microscope. The overall prevalence of infections was 41.9% (201/480). Northwest China had the highest prevalence (70%), followed closely by Northeast China (65%) and Southwest China (62.5%). The prevalences in North China (34%) and South China (25.8%) were significantly lower. The large and medium farms had lower prevalences (34.2% and 37.2%, respectively) than the small farms (61.4%). Coccidian oocysts were found in 42.2% (76/180) of faecal samples from meat rabbits, 40% (28/70) from angora rabbits and 44.7% (85/190) from Rex rabbit. In total, ten species of Eimeria were identified from oocyst-positive samples. Concurrent infection with two to eight Eimeria species was found. E. perforans was the most prevalent species (35.2%), followed in order by E. media, E. magna, E. irresidua and E. intestinalis with prevalences of 31.3%, 28.8%, 19.4%, and 14.8%, respectively. Taken together, These results reveal the characteristics of the prevelance of rabbit coccidia infection in China, including the distribution, the scale of farming and the species, which are indispensable to the control of rabbits coccidiosis in China.

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Section: Sampling and Treatment Methodsmentioning

confidence: 99%

Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China

et al. 2011

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“…Finally, these findings were brought into the infection site and used for identification of Eimeria spp. (Soulsby 1982;Carvalho et al 2011). …”

Section: Laboratory Examinationmentioning

confidence: 99%

Prevalence and risk factors for subclinical coccidiosis in broiler chicken farms in Mazandaran province, Iran

Shirzad

Seifi

Gheisari

et al. 2011

Trop Anim Health Prod

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Coccidiosis is a disease of almost universal importance in Poultry production. The subclinical form of coccidiosis has most significant economic impact due to impaired growth rate and feed conversion. Some factors such as: age, size of flock, season, etc., may effect on severity of this disease. In this study, the relationships between some major risk factors and prevalence of subclinical coccidiosis were investigated. This study was done in 120 broiler farms in five different cities of Mazandaran province, north of Iran. Five chicks (3-8 weeks of ages) were taken randomly from every 120 farms and post-mortem and parasitological examinations were performed. Five Eimeria spp. were recognized: Eimeria tenella, Eimeria maxima, Eimeria acervulina, Eimeria brunetti, and Eimeria necatrix. The prevalence rate of subclinical coccidiosis among them is 75% (90 farms out of 120). E. acervulina was the most prevalent species (65.5%) followed by E. maxima (17.7%), E. tenella (15.5%), E. brunetti (10%), and E. necatrix (5.5%). According to what the results approve, the occurrence of subclinical coccidiosis is significantly related to the age and size of flock, whereas the other factors such as the season of year, industrial strains, chicken's keeping system, and anticoccidial drugs do not affect this phenomenon remarkably.

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“…Oocysts were recovered from the feces after seven days of sporulation, by means of centrifugation in Sheather's solution. The oocysts were photographed for morphological analysis, under an Olympus BX 51 microscope coupled with an Olympus DP71 camera, using the Image-Pro Express 6 software for measurements (CARVALHO et al, 2011). Morphological identification was performed using oocyst length, width, shape index and inner features (DUSZYNSKI & WILBER, 1997).…”

Section: Methodsmentioning

confidence: 99%

Coccidia of gallinaceous meat birds in Brazil

Teixeira

Melo

Albuquerque

et al. 2015

Rev. Bras. Parasitol. Vet.

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Coccidiosis is a disease that limits the production and marketing of gallinaceous birds in North America, especially quails, pheasants and chukar partridges. Virtually no research has been conducted in South America on the causative agents of diseases among these birds, including coccidia. The aim of this work was to make first observations on Eimeria spp. in the chukar partridge Alectoris chukar and the grey quail Coturnix coturnix, which are reared for meat in Brazil. Fecal and tissue samples were collected from commercial farms and were examined for oocysts, gross and microscopic lesions or endogenous stages. From this examination, it was found that partridges raised in Brazil did not have any visible infection. However, grey quails presented mild infection and two Eimeria species that had previously been described in other birds were identified.Keywords: Eimeria, infection, diagnosis, oocyst, morphology. ResumoA coccidiose é uma enfermidade limitante para a produção e comercialização de aves de corte na América do Norte, principalmente codornas, faisões e a perdiz de chukar. Praticamente nenhuma pesquisa foi realizada na América do Sul sobre os agentes causadores de doenças nessas aves, incluindo coccídios. O objetivo deste trabalho foi realizar as primeiras observações sobre Eimeria spp. em perdiz de chukar Alectoris chukar e codornas cinzentas Coturnix coturnix criadas para abate no Brasil. Amostras de fezes e de tecidos foram coletadas em granjas comerciais e examinadas para oocistos, lesões macroscópicas e microscópicas ou estágios endógenos. Após o exame, verificou-se que a criação de perdizes, no Brasil, não tinha infecção visível. No entanto, as codornas cinzentas apresentaram uma infecção leve e foram identificadas duas espécies de Eimeria descritas anteriormente em outras aves.Palavras-chave: Eimeria, infecção, diagnóstico, oocisto, morfologia.

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Diagnosis of Eimeria species using traditional and molecular methods in field studies

Cited by 61 publications

References 22 publications

Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China

Large-scale survey of the prevalence of Eimeria infections in domestic rabbits in China

Prevalence and risk factors for subclinical coccidiosis in broiler chicken farms in Mazandaran province, Iran

Coccidia of gallinaceous meat birds in Brazil

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