Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Parkinson, Christina M.; O'Brien, Alexandra; Albers, Theresa M.; Simon, Meredith A.; Clifford, Charles B.; Pritchett-Corning, Kathleen R.

doi:10.3791/2767

Cited by 10 publications

(13 citation statements)

References 3 publications

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“…Blood parasites were detected by thin and thick blood smear tests. The parasitological methods are in accordance with studies by Parkinson et al (2011). The morphology of the endoparasites were identified using a compound microscope at magnifications of 10× and 40× objectives.…”

Section: Methodsmentioning

confidence: 99%

Stocking Density Associated with Parasitic Infection by Gastrointestinal Examination in Laboratory Mice

Ain-Fatin¹,

Nur-Fazila²,

Nur-Mahiza³

et al. 2020

Adv. Anim. Vet. Sci.

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Recommendation of cage sizes is made based on the Short Communication Abstract | Parasitic infection in the laboratory mice (Mus musculus) may affect certain research outcomes especially during high worm burden. This study allows the assessment of parasite infection of mice based on stocking density that potentially serve as a guideline for proper management to be implemented. Fifty-four (54) male BALB/c mice were randomly chosen from an animal facility located in Klang Valley, Malaysia and placed for three replicates in groups of 3, 6 and 9 mice per cage to reflect different stocking densities. Endoparasites were examined by direct faecal smear, perianal tape test, faecal floatation and gastrointestinal examination techniques. Ectoparasites were identified under fur pluck method, tape impression test and carcass immersion while blood smear techniques were performed for blood parasites detection. Samples were taken weekly for a total of 5 weeks. Results revealed infection with pinworms; Syphacia obvelata and Aspiculuris tetraptera. Statistical analysis revealed no association between parasites and various stocking density using repeated-measures ANOVA. However, gastrointestinal examination mentioned as the 'gold standard' revealed an association using one-way ANOVA when P < 0.05. Overall, the results varied according to the parasitological methods used and stocking densities do not play a role in the parasitic levels of laboratory mice.

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Section: Methodsmentioning

confidence: 99%

Stocking Density Associated with Parasitic Infection by Gastrointestinal Examination in Laboratory Mice

Ain-Fatin¹,

Nur-Fazila²,

Nur-Mahiza³

et al. 2020

Adv. Anim. Vet. Sci.

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“…Paper tape samples were evaluated under a dissection microscope at 400× magnification as described by Parkinson et al . [11]. External parasites were identified based on morphological characteristics and identifications described by Richard and David [12] using 30-400× magnification by microscopy.…”

Section: Methodsmentioning

confidence: 99%

Ectoparasites of brown rats (Rattus norvegicus) in Grenada, West Indies

et al. 2019

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Background and Aim:Arthropod ectoparasites (mites, lice, ticks, and fleas) on common house rats or brown rats (Rattus norvegicus) are known to transmit zoonotic pathogens and diminish rat health. To the best of our knowledge, there is no published information regarding the prevalence of ectoparasites on R. norvegicus in Grenada. This study aimed to determine the prevalence and types of ectoparasites present on brown rats from Grenada.Materials and Methods:One hundred sixty-eight rats were collected live from the parishes of St. George and St. David, Grenada, from May to July 2017. Following euthanasia, external parasites were collected using fine combs, thumb forceps, and paper tape. Tape samples and free specimens were placed in containers with 70% ethanol. External parasites were evaluated using dissection microscopy.Results:Over 2000 ectoparasites were collected from 149 of the 168 trapped rats (88.7%). Ectoparasites identified included mesostigmatid mites (found on 84.6% of infested rats), fur mites in the families Atopomelidae and Listrophoridae (67.1%), Polyplax spp. lice (6.7%), fleas (3.4%), an unidentified larval tick (0.7%), and a mite in the family Myobiidae (0.7%). Infestation rates were higher in St. David Parish (86/89; 96.6%) than in St. George Parish (63/79; 79.7%) (p=0.001). When comparing sex and age, males had a marginal increase in infestation rate (83/89; 93.3%) compared to females (66/79; 83.5%) (p=0.054), and adults had an infestation rate of 90.7% (97/107) compared to juvenile rats who had a 66.7% (14/21) infestation rate.Conclusion:Brown rats in Grenada are heavily infested with ectoparasites, including known vectors of pathogens. Future studies will examine the prevalence of zoonotic pathogens in these arthropods.

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“…Parasitological tests were performed to isolate intestinal helminths and protozoa. Parasitological tests were used fecal flotation and fecal centrifugation concentration (FCC) by zinc sulfate (specific gravity: 1.18) method (5). We differentiated Entamoeba histolytica, G iardia spp .…”

Section: Methodsmentioning

confidence: 99%

Detection of Chilomastix mesnili in Common Marmoset (Callithrix jacchus) and Treatment with Metronidazole

Jeong

Park

Ryu

et al. 2019

IJPA

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Background: Recently, the use of common marmoset (Callithrix jacchus) has increased in biomedical research as an animal model. This study aimed to test fecal samples to monitor bacterial and parasite infections in common marmoset at the Laboratory Animal Center of Osong Medical Innovation Foundation in Korea. Methods: To monitor bacteria and parasites in common marmoset, we tested 43 fecal samples of 43 common marmosets by culture and parasitological test in 2014. Infection by Chilomastix mesnili was determined by PCR method. Results: We identified nonpathogenic bacteria such as Proteus mirabilis and Escherichia coli in feces of normal common marmosets. Interestingly, C. mesnili was isolated from a healthy common marmoset by fecal centrifugation concentration and PCR. The monkey infected with C. mesnili was treated with metronidazole. After the treatment, C. mesnili were not found in feces using fecal centrifugation concentration and PCR. Conclusion: This is the first case report of C. mesnili infection in common marmoset. Treatment with metronidazole is found to be highly effective in eradicating C. mesnili infection in common marmoset.

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Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Cited by 10 publications

References 3 publications

Stocking Density Associated with Parasitic Infection by Gastrointestinal Examination in Laboratory Mice

Stocking Density Associated with Parasitic Infection by Gastrointestinal Examination in Laboratory Mice

Ectoparasites of brown rats (Rattus norvegicus) in Grenada, West Indies

Detection of Chilomastix mesnili in Common Marmoset (Callithrix jacchus) and Treatment with Metronidazole

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