Diagnosis of different liver fibrosis characteristics by blood tests in non-alcoholic fatty liver disease

Calès, Paul; Boursier, Jérôme; Chaigneau, Julien; Lainé, Fabrice; Sandrini, J.; Michalak, Sophie; Hubert, I.; Dib, Nina; Oberti, Frédéric; Bertrais, Sandrine; Hunault, Gilles; Cavaro‐Ménard, Christine; Gallois, Yves; Deugnier, Yves; Rousselet, Marie Christine

doi:10.1111/j.1478-3231.2010.02314.x

Cited by 45 publications

(40 citation statements)

References 28 publications

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“…Patterns of nonlinear scaling rules define fractals, which can be measured in terms of complexity and homogeneity. Fractal geometry has been used to quantitate biological processes, such as tumor boundaries, vascular architecture, angiogenesis (8,10,23,24), bone tissue organization during regeneration (39), and liver fibrosis in rats and humans (5,12,25). Fractal analysis of collagen images provided quantitative measurements for the morphological observations.…”

Section: Discussionmentioning

confidence: 99%

“…An indirect approach to estimate chronic pancreatic damage is the determination of elements actively involved in fibrogenesis, such as cytokines and growth factors (TGF-␤, PDFG, and monocyte chemoattractant protein) and cytofilaments overexpressed by activated PSCs (␣-SMA). Quantitative ECM assessment by morphometry has gained credit to evaluate fibrosis burden because it may better reflect ECM deposition than other parameters (5). In addition, the increased complexity of collagen in fibrosis can be quantitated by fractal analysis (12,25).…”

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confidence: 99%

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Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis

González

García

Samper

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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González AM, Garcia T, Samper E, Rickmann M, Vaquero EC, Molero X. Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis. Am J Physiol Gastrointest Liver Physiol 301: G846 -G855, 2011. First published August 18, 2011 doi:10.1152/ajpgi.00485.2010.-Tocotrienols exhibit anti-inflammatory properties over macrophages and promote cytotoxicity in activated pancreatic stellate cells, suggesting that they may limit chronic pancreatitis progression. We aimed to quantitate the effect of oral tocotrienols on a rat model of chronic pancreatic injury. Chroniclike pancreatitis was induced by repeated arginine pancreatitis. Palm oil tocotrienol-rich fraction (TRF) was given by gavage before and after pancreatitis inductions. Amylase and hydroxyproline were determined in pancreatic homogenates; collagen, fibronectin, ␣-smooth muscle actin (SMA), glial fibrillary acidic protein (GFAP), and phosphorylated Smad3 were assessed by Western blotting. Transforming growth factor (TGF)-␤1 was measured in plasma. Morphological assessment included light microscopy, fibrosis area fraction, and collagen network fractal analysis. Arginine pancreatitis induced pancreatic atrophy and increased hydroxyproline that ameliorated after TRF. Arginine increased TGF-␤1 (185 Ϯ 40 vs. 15 Ϯ 2 ng/ml; P Ͻ0.01) that was blunted by TRF (53 Ϯ 19; P Ͻ 0.01). TRF reduced protease and Smad3 activation, collagen, and fibronectin. ␣-SMA increased and GFAP diminished in arginine pancreatitis, consistent with long-term stellate cell activation, and TRF reverted these changes to basal. Arginine pancreatitis increased fibrosis area fraction (4.5 Ϯ 0.3% vs. 0.2 Ϯ 0.2%), collagen network complexity (fractal dimension 1.52 Ϯ 0.03 vs. 1.42 Ϯ 0.01; P Ͻ 0.001), and inhomogeneity (lacunarity 0.63 Ϯ 0.03 vs. 0.40 Ϯ 0.02; P Ͻ 0.001), which were all reduced by TRF (1.3 Ϯ 0.4%, 1.43 Ϯ 0.02%, and 0.51 Ϯ 0.03%, respectively; P Ͻ 0.01). Best correlation coefficients were obtained when comparing fibrosis area fraction with lacunarity (r ϭ 0.88) and both parameters with pancreatic weight (r ϭ Ϫ0.91 and Ϫ0

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis

González

García

Samper

et al. 2011

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…However, the METAVIR system was also used in NAFLD and was thought to be a better reference for noninvasive panels based on blood tests. 34 Another major issue relates to whether changes in P2/MS scores correspond to changes in hepatic fibrosis over time. In the present study, the sensitivity of the P2/MS to changes in hepatic fibrosis was not examined, and thus, further studies should be undertaken to determine if changes in P2/MS can be utilized to monitor hepatic fibrosis in patients with NAFLD.…”

Section: Patient Characteristicsmentioning

confidence: 99%

Validation of P2/MS and Other Noninvasive Fibrosis Scoring Systems in the Korean Population with Nonalcoholic Fatty Liver Disease

Kim

Lee

et al. 2011

Korean J Gastroenterol

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“…Ils sont importants, car la normalité de la biologie standard n'élimine pas des lésions de fibrose hépatique [20,40].…”

Section: Les Tests Biologiquesunclassified

La stéato-hépatite non alcoolique - Une affection métabolique aux multiples facettes et un challenge hépatologique

Béorchiõã¹

2014

Hegel

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RésuméLa stéato-hépatite non alcoolique demeure une affection hépatique, dont le diagnostic reste sous-estimé et dont le pronostic s'est affiné ces dernières années.Après avoir rappelé les dernières données épidémiologiques, intimement liées à l'obésité et au syndrome métabolique, physiopathologiques, notamment l'axe intestin/ foie, l'auteur aborde la place des marqueurs indirects de fibrose dans l'exploration de toute stéatose hépatique. L'alliance de tests biologiques plus performants, des données de l'élastométrie hépatique et du cap-system s'avère prometteuse. Ceux-ci devraient prochainement remplacer, comme dans l'hépatite C, le rôle central et actuel de la ponction biopsie hépatique.La prise en charge rappelle les difficultés thérapeutiques où l'abord nutritionnel et le style de vie restent primordiaux. Une collaboration avec les endocrinologues/nutritionnistes et les cardiologues demeure la clé de voûte pour optimiser la prise en charge de ces patients. Mots-clés

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Diagnosis of different liver fibrosis characteristics by blood tests in non-alcoholic fatty liver disease

Abstract: Different characteristics of fibrosis in NAFLD can be diagnosed and quantified by blood tests with excellent accuracy.

Cited by 45 publications

References 28 publications

Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis

Assessment of the protective effects of oral tocotrienols in arginine chronic-like pancreatitis

Validation of P2/MS and Other Noninvasive Fibrosis Scoring Systems in the Korean Population with Nonalcoholic Fatty Liver Disease

La stéato-hépatite non alcoolique - Une affection métabolique aux multiples facettes et un challenge hépatologique

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