Diagnosis of Coliform Infection in Acutely Dysuric Women

Abstract

We reevaluated conventional criteria for diagnosing coliform infection of the lower urinary tract in symptomatic women by obtaining cultures of the urethra, vagina, midstream urine, and bladder urine. The traditional diagnostic criterion, greater than or equal to 10(5) bacteria per milliliter of midstream urine, identified only 51 per cent of women whose bladder urine contained coliformis. We found the best diagnostic criterion to be greater than or equal to 10(2) bacteria per milliliter (sensitivity, 0.95; specificity, 0.85). Although isolation of less than 10(5) coliforms per milliliter of midstream urine has had a low predictive value of previous studies of asymptomatic women, the predictive value of the criterion of greater than or equal to 10(2) per milliliter was high (0.88) among symptomatic women the prevalence of coliform infection exceeded 50 per cent. In view of these findings, clinicians and microbiologists should alter their approach to the diagnosis and treatment of women with acute symptomatic coliform infection of the lower urinary tract.

Profile of urinary tract infection in diabetic patients. Data from a general hospital at Jammu and Kashmir, India

“…This study confirmed that the prevalence of UTI among female diabetics was higher when compared to prevalence in males (6).Bacteriological study reveals the involvement of gram -ve enteric organisms that commonly cause UTI such as E coli , Klebsiella species and Proteus species [9,[19][20][21][22][23][24][25].…”

Standardization of isothermal microcalorimetry in urinary tract infection detection by using artificial urine

“…Its high sensitivity in the order of 0.2 lW allows detection of 10 2 active microorganisms per sample. This is even more important as studies comparing voided urine specimens and bladder aspirate specimens in women with UTI have shown that the traditional criterion for a positive culture of voided urine (10 5 CFU/mL) is insensitive for cystitis, and 30-50 % of women with bladder infection have colony counts of 10 2 -10 4 CFU/mL in voided urine [18], a range that is mostly covered by IMC. In our present study conditions (i.e., artificial urine), the high sensitivity of isothermal microcalorimetry (IMC) allowed the rapid detection of all urinary tract pathogens (UTP) investigated.…”

Cystitis: Symptomatology in Women with Suspected Uncomplicated Urinary Tract Infection

“…This finding adds to the ongoing discussion about the most appropriate cutoff value. 5,6 Strengths and limitations To our knowledge, this is the first study investigating the correlation between the severity of symptoms or bacteriuria and the duration of symptoms. The design of the diary was based on previous qualitative and cross-sectional research to maximize its validity.…”

“…Furthermore, although a bacterial culture of > 100,000 colony-forming units (CFU)/mL is generally accepted as defining an infection of the urinary tract, many authors describe the same symptoms in patients with lower levels of bacteriuria and even in culture-negative women. [4][5][6][7] It has even been suggested that antibiotic therapy was effective in culture-negative women. 8 These conflicting observations demonstrate that much is unclear about the clinical evolution of signs and symptoms in women with suspected cystitis who are receiving antimicrobial treatment.…”