Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis

Nouri, Hamid Reza; Sankian, Mojtaba; Vahedi, Fatemeh; Afsharzadeh, Danial; Rouzbeh, Leila; Moghadam, Malihe; Varasteh, Abdolreza

doi:10.1007/s11033-011-1083-9

Cited by 23 publications

(20 citation statements)

References 31 publications

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“…About 40 members of this protein family have been identified to date. Fewer than 10% of pollen-allergic patients are sensitized to these minor allergens [8], although this percentage may vary among individuals sensitized to specific pollen sources [1,[9][10][11]. The high degree of sequence homology in polcalcins underlies their extensive crossreactivity [12][13][14], although Phl p 7 (the grass polcalcin) seems to be the strongest IgE binder [12].…”

Section: Introductionmentioning

confidence: 99%

IgE Reactivity to Polcalcins Varies According to Pollen Source

Asero¹,

Mistrello²,

Amato³

2016

J Investig Allergol Clin Immunol

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Background: Polcalcins are highly cross-reactive pollen panallergens. Less than 10% of allergic patients are sensitized to polcalcins. All pollen species are considered able to sensitize patients to this panallergen. Objective: We aimed to assess the presence of polcalcins in various pollen extracts used in allergen immunotherapy. Methods: ELISA inhibition experiments were performed with sera from patients sensitized to polcalcin and rPhl p 7 and rBet v 4. Recombinant polcalcin was used as the substrate and freshly prepared pollen extracts as inhibitors. Results: All pollen extracts induced significant inhibition of IgE reactivity to rBet v 4, whereas only grass pollen extract induced marked inhibition of IgE reactivity to rPhl p 7. Conclusion: Grass polcalcin probably contains more epitopes than polcalcins from other pollen sources. Grass pollen could be responsible for sensitization to polcalcins, and grass pollen immunotherapy is likely to be an option for polcalcin-hypersensitive patients.

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Section: Introductionmentioning

confidence: 99%

IgE Reactivity to Polcalcins Varies According to Pollen Source

Asero¹,

Mistrello²,

Amato³

2016

J Investig Allergol Clin Immunol

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“…Recombinant (r)Che a 2, a major allergen in Chenopodium album pollen, was expressed and purified from Escherichia coli BL21 (DE3) by metal affinity chromatography, as described in our previous study (19). The alum gel suspension was purchased from Sigma-Aldrich (St. Louis, MO, USA).…”

Section: Methodsmentioning

confidence: 99%

Elevated caspase-1 activity and IL-1β expression are associated with the IPAF inflammasome in an experimental model of allergy

Nouri

Karkhah

Mohammadzadeh³

et al. 2016

Molecular Medicine Reports

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Abstract. Previous studies have indicated that interleukin (IL)-1β has an important role in the development of allergic diseases. Therefore, the present study aimed to investigate the upstream pathway underlying IL-1β production in an experimental model of allergy. BALB/c mice (female, 6-8 weeks old) were sensitized to recombinant (r) Che a 2 by intraperitoneal injection of rChe a 2 adsorbed onto an alum gel suspension on days 0, 7, 14 and 21. In the control group, mice received an injection of 20 mM phosphate-buffered saline absorbed onto alum via the same route. The allergic status of the mice was confirmed serologically by measuring allergen-specific immunoglobulin (Ig)E levels. The protein expression levels of IL-1β and the mRNA expression levels of inflammasome compartments were measured by enzyme-linked immunosorbent assay and semi-quantitative reverse transcription polymerase chain reaction, respectively. In addition, caspase-1 activity was determined by fluorometric assay. Sensitized mice exhibited significantly increased levels of specific IgE (P<0.05). IL-1β production and caspase-1 activity were significantly higher in the sensitized mice compared with the control group. In addition, no significant differences were observed between the control and sensitized mice in the expression of genes associated with the inflammasome, including NLR family, pyrin domain containing 3; apoptosis-associated speck-like protein; and NLR family, apoptosis inhibitory protein 5. However, IL-1β converting enzyme protease-activating factor (IPAF) expression was significantly increased in sensitized mice compared with in the control group (P<0.05). These data indicate that caspase-1 activation and IL-1β expression are associated with the IPAF inflammasome. Therefore, based on this association, the IPAF inflammasome may be considered for IL-1β production in the experimental model of allergy. IntroductionAn allergic response is characterized by an exaggerated immune reaction to certain antigens, including harmless environmental antigens from various sources, such as food and pollen. Approximately 25% of the population in developed countries suffers from immunoglobulin (Ig)E-mediated type I allergy (1,2). Interleukin (IL)-1β has been identified as an important cytokine, which has a key role in the pathophysiology of allergic disorders (3). Furthermore, IL-1β has an essential role in the immune response to infectious agents (4).IL-1β can be produced by immune cells, including blood monocytes, tissue macrophages and dendritic cells (5). IL-1β secretion is a two-step process: Firstly, interaction of Toll-like receptors (TLRs) with their ligands leads to an upregulation of pro-IL-1β gene transcription via nuclear factor-κB (6). Secondly, caspase-1 activation results in the conversion of the immature pro-IL-1β into mature IL-1β. It is well-established that caspase-1 activity is regulated by a cytosolic multi-protein complex known as the inflammasome (7). The inflammasome consists of a nucleotide-binding domain like receptor (NLR), o...

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“…Immunologic experiments confirmed that the rHM had low capacity of IgE binding while its immunogenicity was preserved [16]. The recombinant Che a 1, Che a 2 and Che a 3 were previously expressed and purified from E. coli [13] and we had built an allergenic cocktail with equimolar concentrations of them. The allergenic cocktail concentration was adjusted to 5 µg.…”

Section: Methodsmentioning

confidence: 99%

“…The allergenic cocktail concentration was adjusted to 5 µg. Eight C. album -allergic patients that simultaneously showed high levels of specific IgE against rChe, rChe a 2 and rChe a 3 were included in this study and a pooled serum was prepared for competitive ELISA [13]. Female BALB/c mice (6 weeks old) were purchased from Razi Vaccine and Serum Research Institute (Mashhad, Iran).…”

Section: Methodsmentioning

confidence: 99%

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Immunotherapy with a Recombinant Hybrid Molecule Alleviates Allergic Responses More Efficiently than an Allergenic Cocktail or Pollen Extract in a Model of <b><i>Chenopodium album</i></b> Allergy

Nouri

Sankian²,

Afsharzadeh³

et al. 2013

Int Arch Allergy Immunol

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Background: The aim of this study is to assess the therapeutic potential of a recombinant hybrid molecule (rHM) alongside an allergenic cocktail from recombinant wild-type allergens as well as pollen extract on Chenopodium album allergy, using a BALB/c mouse model. Methods: The BALB/c mice had already been sensitized to C. album via intraperitoneal injections of alum-adsorbed allergenic cocktail and immunotherapy procedure was followed by subcutaneous injections of the rHM, allergenic cocktail and pollen extract at weekly intervals. Humoral immune responses were determined via measurement of specific antibodies in serum. Splenocytes of immunized mice were stimulated in vitro and then proliferation responses, cytokine secretion and mRNA expression of genes involved in immunotherapy were examined by ELISA and real-time PCR. Results: Sensitized mice were identified with high specific IgE against allergenic cocktail when compared with healthy mice. Immunotherapy with the rHM induced the highest ratio of the IgG2a/IgG1 levels compared to allergenic cocktail or C. album pollen extract. The rHM was able to induce proliferative responses as well as the allergenic cocktail in cultured splenocytes. Immunotherapy with the rHM significantly improved secretion of IFN-γ and IL-10, while secretion of IL-13 rapidly diminished. Interestingly, mRNA expression of GATA3 was strongly decreased in rHM-treated mice whereas mRNA expression of T-bet and Foxp3 was significantly increased. Conclusion: Our results prove that immunotherapy with the rHM effectively controlled allergic responses by shifting from a Th2-like immune response to a Th1-dominated immune response.

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Diagnosis of Chenopodium album allergy with a cocktail of recombinant allergens as a tool for component-resolved diagnosis

Cited by 23 publications

References 31 publications

IgE Reactivity to Polcalcins Varies According to Pollen Source

IgE Reactivity to Polcalcins Varies According to Pollen Source

Elevated caspase-1 activity and IL-1β expression are associated with the IPAF inflammasome in an experimental model of allergy

Immunotherapy with a Recombinant Hybrid Molecule Alleviates Allergic Responses More Efficiently than an Allergenic Cocktail or Pollen Extract in a Model of <b><i>Chenopodium album</i></b> Allergy

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