Diagnosis of canine echinococcosis: comparison of coproantigen and serum antibody tests with arecoline purgation in Uruguay

Craig, P. S.; Gasser, Robin B.; Parada, Luis F.; Cabrera, P.; S, Parietti; Borgues, C.; Acuttis, A.; Agulla, J.; Snowden, Kimberley C.; Paolillo, Emily W

doi:10.1016/0304-4017(94)00680-b

Cited by 102 publications

(75 citation statements)

References 12 publications

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“…Thus, a negative result confirms that a dog is either truly noninfected or harbors a very low worm burden infection, both conditions are epidemiologically not significant. The specificity of this test was high (91.0 %) and is comparable to what has been reported earlier (Allan et al, 1992;Deplazes et al, 1992;Craig et al, 1995).…”

Section: Discussionsupporting

confidence: 90%

“…Serodiagnosis is accom panied with false negative results at the commence ment of infection and false positive ones due to cross reactivity with other helminth infections Rickard, 1985 andGasser et al, 1988 and1993). The sandwich enzyme linked immunosorbent assay (ELISA) has recently been applied for diagnosis of E. granulosus and E. multilocularis in canines through the detection of coproantigens (Allan et al, 1992;Deplazes et al, 1992 and1994;Craig et al, 1995;Nonaka et al, 1996;Malgor et al, 1997;Ahmad & Nizami, 1998). The coproantigen test has the advan tage of early detection of the infection during prepatency, in 4-10 days post-infection (Deplazes et al, 1992).…”

Section: U Nilocular Hydatidosis or Cystic Echinococcosis (Ce) Is A Cmentioning

confidence: 99%

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Diagnosis of canine echinococcosis: comparison of coproantigen detection with necropsy in stray dogs and red foxes from northern Jordan

et al. 2000

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Summary:The sandwich enzyme linked immunosorbent assay (ELISA) was used as a diagnostic test for Echinococcus granulosus infection by detecting coproantigens in 94 stray dogs Corn's familiaris and eight red foxes (Vu/pes vulpes) from northern Jordan. The results were analyzed in relation to actual helminth infection as revealed by necropsy. The infection rate of dogs with E. granulosus was 1 3.8 % with a worm load ranging between 3 -> 10,000 per infected dog. In contrast, eight of 1 3 E. granulosus infected dogs were coproantigen positive (overall sensitivity 61.5 %). The sensitivity increased to 87.5 % and 100 % in dogs harboring > 20 and > 100 worms/dog, respectively. The specificity of coproantigen-ELISA was 91 %. The greatest cross-reactivity was found in dogs infected with Dipylidium caninum. The positive and negative predictive values for the coproantigen-ELISA test were 50 % and 94.2 %, respectively. Thus, a coproantigen negative dog is most probably truly negative for E. granulosus. In contrast, a coproantigen positive dog may not be truly positive for E. granulosus, except if it has a high worm burden of > 1 00 worms/animal. KEY WORDS :

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Section: Discussionsupporting

confidence: 90%

Section: U Nilocular Hydatidosis or Cystic Echinococcosis (Ce) Is A Cmentioning

confidence: 99%

Diagnosis of canine echinococcosis: comparison of coproantigen detection with necropsy in stray dogs and red foxes from northern Jordan

et al. 2000

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“…In the present study the sensitivity of CA-ELISA was 72.1 %, which was higher than as reported by Zariffard et al, 1999 (68 %). Studies with different types of CA-ELISAs have revealed sensitivities for E. granulosus detection of 88 % in Peru (Lopera et al, 2003), of 83 % in Cyprus (Christofi et al, 2002), of 77 % in Uruguay (Craig et al, 1995), of 63 % in Spain (Deplazes et al, 1994) and of 62 % in Jordan (El-Shehabi et al, 2000). Various studies have shown that the sensitivity of CA -ELISA is strongly dependent on the worm burden.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a coproantigen enzyme-linked immunosorbent assay for the diagnosis of canine echinococcosis in Iran

Siavashi

Motamedi

2006

Helminthologia

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Echinococcosis is one of the most important zoonosis in Iran. Due to this fact that providing a reliable diagnostic method for detection of this infection in definitive host is a critical prerequirement for the establishment of appropriate control programs in our country, one hundred and sixteen carnivores including 80 dogs, 27 jackals, 8 foxes and one wolf were collected from rural areas of Hamadan, Azarbaijan and Tehran provinces and examined for Echinococcus granulosus infection. Canine echinococcosis was diagnosed upon direct microscopic examination of intestinal contents and mucosal scraping for adult tapeworms, and a coproantigen detection enzyme linked immunosorbent assay (CA-ELISA) for Echinococcus granulosus. The overall prevalence of canine echinococcosis using the ELISA test was 43.1 % (50/116). The relative frequency of canine echinococcosis was 37 % (43/116) by microscopic examination. The sensitivity and specificity of the CA-ELISA test as referenced by necropsy findings was 72.1 % and 74 % respectively. We found this assay to be a very suitable and advantageous method for the surveillance of canine population especially in regions with endemic echinococcosis.

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“…Two major diagnostic methods have been used in dogs extensively. These are purgation with arecoline compounds and necropsy (Unruh et al, 1973;Craig et al, 1995;Eckert et al, 2001).…”

Section: Diagnosis Of E Granulosus In Dogsmentioning

confidence: 99%

“…The technique showed 100% specificity, but has certain limitations due to its poor sensitivity, as not all infected dogs respond to the purge and eliminate parasites. In addition to this, the technique is bio-hazardous, time consuming and must be administered by trained personnel (Craig et al, 1995;Eckert et al, 2001). Moreover, most of the epidemiological data and models have been developed from the results of this method (Torgerson et al, 2003).…”

Section: Diagnosis Of E Granulosus In Dogsmentioning

confidence: 99%

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

Mohamed¹,

Wafa²,

Kawther³

et al. 2017

J. Vet. Med. Anim. Health

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Echinococcus granulosus is a tiny tapeworm that parasitizes the small intestine of canids, mainly dogs, which act as definitive hosts for the parasite. Infected dogs are the main source of infection to humans and livestock which act as intermediate hosts resulting in hydatid disease condition. E. granulosus is widely distributed in many parts of the world, and is very common in North African countries. In Libya, the rate of infection with echinococcosis in dogs was reported to be lower than 7 to 80% in stray dogs, 34.8 to 60% in sheep/guard dogs and 7.7 to 21.6% in farm/house dogs. This data fulfills the world health organization (WHO) criteria and suggests that the incidence of infection with echinococcosis/ hydatidosis in some parts of the country can be reaching the level of hyper endemic. Diagnosis of echinococcosis in infected dogs can be performed by isolating the parasite from their faeces or from the contents of their small intestine after necropsy. Recent developments in immunodiagnostic assays for echinococcosis in dogs have been described. Public health and risk factors as well as ways of hydatid disease treatment and various control strategies, including the use of veterinary vaccines, have also been discussed.

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Diagnosis of canine echinococcosis: comparison of coproantigen and serum antibody tests with arecoline purgation in Uruguay

Cited by 102 publications

References 12 publications

Diagnosis of canine echinococcosis: comparison of coproantigen detection with necropsy in stray dogs and red foxes from northern Jordan

Diagnosis of canine echinococcosis: comparison of coproantigen detection with necropsy in stray dogs and red foxes from northern Jordan

Evaluation of a coproantigen enzyme-linked immunosorbent assay for the diagnosis of canine echinococcosis in Iran

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

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