Diagnosis of Brucellosis in Livestock and Wildlife

Godfroid, Jacques; Nielsen, Klaus; Saegerman, Claude

doi:10.3325/cmj.2010.51.296

Cited by 248 publications

(227 citation statements)

References 45 publications

(52 reference statements)

Supporting

Mentioning

218

Contrasting

Unclassified

Order By: Relevance

“…As a consequence, in known infected herds, animals classified positive by iELISA and negative by RBT and/or CFT, should be considered as infected [11]. The results in our study are consistent with this observation.…”

Section: Evaluating the Brucellin Skin Test As An Additional Test To supporting

confidence: 82%

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

Nyanhongo¹

2017

SOJMID

View full text Add to dashboard Cite

Brucellosis is a bacterial disease of high veterinary importance, besides being a significant zoonosis worldwide. It is characterised by variable, and at times long and latent, incubation periods, which makes its control and eradication a challenging effort. No single test is able to identify all stages of the disease with high sensitivity. The brucellin skin test, which has been proved to identify some acute and chronic latent stages of brucellosis, has not been validated in South Africa. The objective of this study was to evaluate the brucellin skin test in brucellosis free, as well as confirmed infected cattle herds, under South African commercial farming conditions. The results indicate that the brucellin skin test has a high specificity (99.2% (95% confidence interval (CI): 98.1% -99.7%)), and that despite a relatively low sensitivity (42.9 % (95% CI: 30.5% -56.0%)), it was able to identify all the infected herds and detect more infected heifers in infected herds when compared to the routinely used Rose Bengal Test and Complement Fixation Test. It was concluded that the brucellin skin test could be used in parallel with serological tests to improve the sensitivity of the current diagnostic strategy in chronically infected herds where eradication of the disease has proven problematic. Removal of more infected heifers would decrease infection pressure and streamline vaccination towards heifers not exposed to Brucella abortus.

show abstract

Section: Evaluating the Brucellin Skin Test As An Additional Test To supporting

confidence: 82%

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

Nyanhongo¹

2017

SOJMID

View full text Add to dashboard Cite

show abstract

“…Abortion in last trimester, birth of unthrifty newborn, retained placenta in female and orchitis, epididyimitis in male animals are the common manifestations of brucellosis in animals [3][4][5] and it may lead to temporary or permanent infertility [6]. Diagnosis on the basis of abortion is, however, equivocal since many pathogens can induce abortion; thus laboratory testing is essential [7]. Various tests viz; Rose Bengal Plate Test (RBPT), Standard Tube Agglutination Test (STAT), Enzyme Linked Immunosorbent Assay (ELISA) and Milk Ring Test (MRT) are commonly used for the detection of anti-Brucella antibody [5].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Kushwaha¹

2014

JMEN

View full text Add to dashboard Cite

Brucellosis is a contagious systemic bacterial disease of livestock. Antibiotics commonly being used have limited efficacy against the disease. In the present study, therapy with antibiotics on Brucella infected cattle was performed and subsequently haemato-biochemical parameters, detection of anti-Brucella antibodies in both serum and milk by different diagnostics and shedding of Brucella in milk were assessed. Treatment with antibiotics; streptomycin and long acting tetracycline alone and in combination were given for four weeks. Hb, TEC, TLC, neutrophils, eosinophils, lymphocytes, monocytes count, AST, ALT and SD values were lower whereas, PCV, serum glucose, total protein, albumin and creatinine were higher in Brucella infected cattle. Treatment with combination therapy showed promising results in these animals. Haemato-biochemical parameters were become near to normal in most of animals that received combination therapy. However, not all animals were returned to normal after treatment but a significant numbers in combination therapy were reduced anti-Brucella antibody titre, both in serum and milk. The level of shedding of Brucella in milk had also gone undetectable. KeywordsBrucellosis; Cattle; Haemato-biochemical parameters; Oxytetracycline; Streptomycin Reaction (PCR) based assays have been developed for the rapid identification of Brucella. It is advantageous to detect DNA of pathogen that rendered biologically safe and hence, reduced the risk of infection to laboratory workers. In addition, PCR is more sensitive than traditional culture method [8].There is limited evidence on treatment aspect of Brucella infected cattle as well as their haemato-biochemical parameters. Brucella is inaccessible to antibiotics as they are facultative intracellular pathogens. Many antimicrobials are active against Brucella species; however, clinical efficacy does not always correlate with in vitro susceptibility [9]. Treatments for brucellosis have been tried previously but none was found successful [10,11]. Streptomycin acts synergistically with penicillin or tetracycline to inhibit the growth of B. abortus within bovine cells cultured in vitro [12]. Considering the above facts, the present work was intended to determine the efficacy of antibiotic therapy and alteration in the haemato-biochemical parameters if any, in Brucella infected cattle after treatment. Materials and MethodsA total of 27 cattle which showed antibodies both in serum and milk as well as Brucella DNA in milk were selected for the study. ELISA, RBPT and STAT were employed on serum whereas, MRT and ELISA on milk to detect the anti-Brucella antibodies. Milk samples were processed for detection of pathogen itself in form of its DNA (16S rRNA) by PCR [13]. Reference antigens and serum for RBPT, STAT and, for MRT, Abortus Bang Ring Antigen (ABR Antigen) were procured from the Indian Veterinary Research Institute, Izatnagar (India). AniGen B. Brucella Ab ELISA kit (Cat. No. EB43-01) (i-ELISA and Milk-ELISA) was procured

show abstract

“…In sheep, considerable conditional dependence between iELISA and RBT and between RBT and SAT among infected as well as non-infected sheep were observed. The iELISA is a quantitative test which detects only IgG, SAT quantifies both IgM and IgG (but mainly IgM) and RBT qualitatively detects both IgM and IgG (Christopher et al, 2010;Godfroid et al, 2010;Dı'az et al, 2011). The conditional correlation between RBT and SAT for sheep may be explained by the similarity of the type of antibody detected.…”

Section: Discussionmentioning

confidence: 99%

Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh

Rahman¹,

Saegerman²,

Berkvens³

et al. 2013

Preventive Veterinary Medicine

Self Cite

View full text Add to dashboard Cite

a b s t r a c tThe true prevalence of brucellosis and diagnostic test characteristics of three conditionally dependent serological tests were estimated using the Bayesian approach in goats and sheep populations of Bangladesh. Serum samples from a random selection of 636 goats and 1044 sheep were tested in parallel by indirect ELISA (iELISA), Rose Bengal Test (RBT) and Slow Agglutination Test (SAT). The true prevalence of brucellosis in goats and sheep were estimated as 1% (95% credibility interval (CrI): 0.7-1.8) and 1.2% (95% CrI: 0.6-2.2) respectively. The sensitivity of iELISA was 92.9% in goats and 92.0% in sheep with corresponding specificities of 96.5% and 99.5% respectively. The sensitivity and specificity estimates of RBT were 80.2% and 99.6% in goats and 82.8% and 98.3% in sheep. The sensitivity and specificity of SAT were 57.1% and 99.3% in goats and 72.0% and 98.6% in sheep. In this study, three conditionally dependent serological tests for the diagnosis of small ruminant brucellosis in Bangladesh were validated. Considerable conditional dependence between IELISA and RBT and between RBT and SAT was observed among sheep. The influence of the priors on the model fit and estimated parameter values was checked using sensitivity analysis. In multiple test validation, conditional dependence should not be ignored when the tests are in fact conditionally dependent.

show abstract

Diagnosis of Brucellosis in Livestock and Wildlife

Cited by 248 publications

References 45 publications

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

Evaluating the Brucellin Skin Test as an Additional Test to Control Bovine Brucellosis

Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle

Bayesian estimation of true prevalence, sensitivity and specificity of indirect ELISA, Rose Bengal Test and Slow Agglutination Test for the diagnosis of brucellosis in sheep and goats in Bangladesh

Contact Info

Product

Resources

About