Diagnosis of bacterial infection

Boyles, Tom; Wasserman, Sean

doi:10.7196/samj.9647

Cited by 28 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with this hypothesis, we showed that C. mirare can be cultured on TSA blood agar while C. crescentus cannot, likely due to the increased salt tolerance of C. mirare (Fig 2). Moving forward, culture-independent identification methods such as mass spectrometry and metagenomic sequencing will help determine the true frequency of Caulobacter infections in humans [48,49].…”

Section: Plos Onementioning

confidence: 99%

Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model

Moore

Gitai

2020

PLoS ONE

View full text Add to dashboard Cite

The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes. However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability. Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial. Consequently, we sequenced a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants. We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions. C. mirare phylogenetically resembles both C. crescentus and the related C. segnis, which was also thought to be non-pathogenic. The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent. We tested this hypothesis by characterizing the ability of Caulobacters to infect the model animal host Galleria mellonella. In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic. Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity. Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.

show abstract

Section: Plos Onementioning

confidence: 99%

Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model

Moore

Gitai

2020

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…Early diagnosis of bacterial infections is very crucial and can be life-saving. Accurate diagnosis is important to avoid unnecessary antibiotic use and give the suitable treatment as soon as possible, this requires combining signs and symptoms with diagnostic tests to determine the causative pathogen 1. Methods used in the diagnosis of some diseases can be expensive, inconvenient, time-consuming, and even invasive.…”

Section: Introductionmentioning

confidence: 99%

<p>Evaluating the potential use of electronic tongue in early identification and diagnosis of bacterial infections</p>

Al-Ramahi¹,

Zaid²,

Abu-Khalaf³

2019

IDR

View full text Add to dashboard Cite

Background Electronic tongue (ET) is a simple device that may have some applications in the medical field as an alternative tool to traditional diagnostic methods. The aim of this study is to evaluate the potential use and accuracy of ET in the diagnosis of certain bacterial infections. Methods An alpha-Astree ET was used for the detection of known bacterial strains Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 27853) which were tested at three-time intervals; 15, 18, and 24 hrs (hr). This was used to build a qualitative and quantitative mathematical model to detect the presence of bacteria at the earliest possible time. When the model is robust, new unknown samples were tested after 15 hrs of bacterial growth. Samples were identified using multivariate data analysis techniques. Results Principal Component Analysis (PCA) scores showed that ET can distinguish between the three bacteria at different times 15, 18, and 24 hrs using different sensors. In the PCA scores plots, the discrimination index was 83% at 15 hrs, 88% at 18 hrs, and 96% at 24 hrs, the variances explained by the two principal components were 84%, 99%, and 97% at 15, 18, and 24 hrs, respectively. Fifteen hours was the earliest time at which the bacteria could be detected. Then six samples of E.coli (as unknown samples) were tested after 15 hrs of inoculation, the two discrimination function explained about 100% of the variance (ie, 79.7+22.3%) and all unknown samples were identified as E.coli . Conclusion ET could differentiate between types of bacteria in addition to identifying unknown bacterial cultures ( E. coli ) at times shorter than that required in the current culture-based methods (24–48 hrs), this could be of a great value in early diagnosis of life-threatening infections.

show abstract

“…Although rapid tests, such as through protein evaluation (Procalcitonin and C-reactive protein) may present certain subjectivity and variability according to the patient, it is still a better option than only a clinical diagnosis only [16,19,24].…”

Section: Bibliographymentioning

confidence: 99%

“…In addition to the rapid tests, we must emphasize the importance of microbiology laboratories, which provide specific culture and antibiogram data for each patient, although they are generally used to focus or interrupt empirically initiated treatment [3,19].…”

mentioning

confidence: 99%

Evaluation of Antibiotics Prescriptions in the City of Vitoria, ES - Brazil, and its Relation with Laboratory Diagnosis

Costa¹,

Arpini²

2018

acta scient microb

View full text Add to dashboard Cite

Diagnosis of bacterial infection

Cited by 28 publications

References 19 publications

Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model

Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model

<p>Evaluating the potential use of electronic tongue in early identification and diagnosis of bacterial infections</p>

Evaluation of Antibiotics Prescriptions in the City of Vitoria, ES - Brazil, and its Relation with Laboratory Diagnosis

Contact Info

Product

Resources

About