Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen

Carvalho, Fernando Aécio de Amorim; Charest, Hugues; Tavares, Carlos Alberto Pereira; Matlashewski, Greg; Valente, Eneida P.; Rabello, Ana; Gazzinelli, Ricardo T.; Fernandes, Ana Paula

doi:10.1016/s0732-8893(02)00410-8

Cited by 85 publications

(67 citation statements)

References 20 publications

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“…Antibodies against the A2 protein have also been detected in dogs and humans infected with L. donovani or L. mexicana. However, antibodies to A2 have not been detected in humans with the cutaneous form of the disease (GHEDIN et al, 1997;CARVALHO et al, 2002). This study, in which an A2 protein was isolated from L. infantum, also supports the study of Ghedin et al (1997), in which karyotypic analysis performed in several species of Leishmania showed that A2 genes are conserved in L. infantum.…”

Section: Discussionsupporting

confidence: 78%

“…The A2 recombinant protein from L. donovani has been tested also in vaccine development including a disponible commercial vaccine, Leish-Tec  (CARVALHO et al, 2002;FERNANDES et al, 2008;TESTASICCA et al, 2014). Further research and novel protocols are being developed to obtain a better characterization of this newly expressed recombinant protein.…”

Section: Discussionmentioning

confidence: 99%

“…Charest & Matlashewski (1994) isolated and expressed a protein from the A2 multigene family from L. donovani infantum, which was recognized by 60% and 82% of humans with visceral leishmaniasis (VL) in India and Sudan, respectively, using ELISA. Moreover, Carvalho et al (2002), using a recombinant A2 protein from Leishmania donovani in an ELISA test, found a seroprevalence of 77% in symptomatic VL patients and 87% among positive dogs in Brazil. Genes in the A2 family are abundantly transcribed and translated during the amastigote stage of the L. donovani complex, but are not expressed during the promastigote stage (CHAREST & MATLASHEWSKI, 1994;GHEDIN et al, 1997;ZHANG & MATLASHEWSKI, 2001;FARAHMAND et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test

Jusi

Oliveira

Nakaghi³

et al. 2015

Rev. Bras. Parasitol. Vet.

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This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three different strains of Escherichia coli. We used 29 sera samples from Leishmune-vaccinated dogs, 482 sera samples from dogs from endemic areas (positive controls), and 170 sera samples from dogs from non-endemic areas (negative controls) in ELISA tests using soluble Leishmaniaantigen (SLA) and His-A2 as antigen. Expressed proteins showed, by western blotting, the expression of an 11 KDa protein. Sixty-three percent (303/482) of the samples from endemic areas were positive by ELISA His-A2, whereas 93.1% (27/29) of Leishmune®-vaccinated animals were negative by His-A2-ELISA. Anti-A2 antibodies from mice inoculated with the A2 protein were detected in slides containing amastigote forms, but not in slides containing promastigote forms. The A2 recombinant protein from L. chagasi may be a useful tool in the diagnosis of CVL, and further tests regarding the infection stage and the specie of parasite at which the dogs are sampled should provide a better understanding of our results.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test

Jusi

Oliveira

Nakaghi³

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…The DAT has been used as a reference test in many sero-prevalence studies and is suitable for the sero-diagnosis of canine VL. Taken together, our fi ndings suggest that the rA2 protein may be a valuable tool for the diagnosis of VL in the Old World, as suggested previously for the New World (35) , particularly for the diagnosis of infection in dogs. Because A2 is a recombinant protein, it may also be useful in the development and improvement of other simpler and faster serological tests.…”

Section: Cross-reactionsupporting

confidence: 81%

“…For asymptomatic dogs, rates of 70.5%, 99.1%, and 100% were shown to be positive by PCR, real-time PCR, and DAT, respectively (34) . In a study using rA2-ELISA, anti-A2 antibodies were detected in 14 out of 15 symptomatic dogs and in 10 out of 13 asymptomatic dogs in Brazil (35) . This report from Brazil provided a comparison of ELISA using L. infantum rK26 and rK39 antigens and the L. donovani rA2 protein, as well as ELISA with crude soluble antigen (CSA), indicating a high sensitivity for rK26 and rK39 in the detection of symptomatic dogs (94% and 100%, respectively), followed by CSA (88%) and rA2 (70%).…”

Section: Cross-reactionmentioning

confidence: 99%

Comparison of recombinant A2-ELISA with rKE16 dipstick and direct agglutination tests for diagnosis of visceral leishmaniasis in dogs in Northwestern Iran

Farahmand

Khalaj

Mohebali

et al. 2015

Rev. Soc. Bras. Med. Trop.

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Introduction: Various methods are used for the diagnosis of visceral leishmaniasis (VL), such as microscopic examination, culture and inoculation of laboratory animals; however, serological assays are commonly used for the detection of antibodies in serum samples with a wide range of specifi city and sensitivity. Methods: The purpose of this study was to compare three serological methods, including rA2-ELISA, the recombinant KE16 (rKE16) dipstick test and the direct agglutination test (DAT), for the detection of antibodies against VL antigens. The assays utilized 350 statistically based random serum samples from domestic dogs with clinical symptoms as well as samples from asymptomatic and healthy dogs from rural and urban areas of the Meshkinshahr district, northwestern Iran. Results: Samples were assessed, and the following positive rates were obtained: 11.5% by rKE16, 26.9% by DAT and 49.8% by ELISA. The sensitivity among symptomatic dogs was 32.4% with rKE16, 100% with DAT and 52.9% with ELISA. Conversely, rA2-ELISA was less specifi c for asymptomatic dogs, at 46.5%, compared with DAT, at 88.9%. Conclusions: This study recommends rA2-ELISA as a parallel assay combined with DAT to detect VL infection among dogs. Further evaluations should be performed to develop an inexpensive and reliable serologic test for the detection of Leishmania infantum among infected dogs.

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Vaccines for Canine Leishmaniasis

Coelho

Christodoulides

2023

Vaccines for Neglected Pathogens: Strategies, Achievements and Challenges

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Visceral leishmaniasis is a zoonotic disease in many countries and dogs are considered the main domestic reservoir of Leishmania parasites, and the presence of infected animals represents a potential risk for human disease. In this chapter, we review the state-of-the-art of canine visceral leishmaniasis (CanL) vaccines, discussing the properties and problems associated with the few currently licensed and discontinued vaccines and looking forward to the development of new, more effective vaccines. Reducing the incidence of CanL through vaccination will improve canine health and welfare and contribute to preventing human VL.

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Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen

Cited by 85 publications

References 20 publications

Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test

Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test

Comparison of recombinant A2-ELISA with rKE16 dipstick and direct agglutination tests for diagnosis of visceral leishmaniasis in dogs in Northwestern Iran

Vaccines for Canine Leishmaniasis

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