Diagnosis and Treatment of Mycoplasma‐Contaminated Cell Cultures

Harlin, Helena; Gajewski, Thomas F.

doi:10.1002/0471142956.cya03cs43

Cited by 10 publications

(10 citation statements)

References 9 publications

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“…Given the frequency of misidentification and cross-contamination ( Nardone, 2007 ; Lacroix, 2008 ) it is essential that all cell lines are rigorously checked for their provenance and genetic identity ( Parodi et al , 2002 ; Yoshino et al , 2006 ). It is also important that cell lines are free from contamination with infectious agents such as mycoplasma, which can influence their biological behaviour and present a risk to handlers and animals ( Ishikawa et al , 2006 ; Sung et al , 2006 ; Harlin and Gajewski, 2008 ). Regardless of origin, detailed characterisation of tumours should be performed and checked periodically to ensure that desired properties are maintained and are commensurate with the molecular pathology of the corresponding human malignancy ( Santarius et al , 2010 ).…”

Section: Tumour Modelsmentioning

confidence: 99%

Guidelines for the welfare and use of animals in cancer research

et al. 2010

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Animal experiments remain essential to understand the fundamental mechanisms underpinning malignancy and to discover improved methods to prevent, diagnose and treat cancer. Excellent standards of animal care are fully consistent with the conduct of high quality cancer research. Here we provide updated guidelines on the welfare and use of animals in cancer research. All experiments should incorporate the 3Rs: replacement, reduction and refinement. Focusing on animal welfare, we present recommendations on all aspects of cancer research, including: study design, statistics and pilot studies; choice of tumour models (e.g., genetically engineered, orthotopic and metastatic); therapy (including drugs and radiation); imaging (covering techniques, anaesthesia and restraint); humane endpoints (including tumour burden and site); and publication of best practice.

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Section: Tumour Modelsmentioning

confidence: 99%

Guidelines for the welfare and use of animals in cancer research

et al. 2010

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“…However, the representativity of the cancer cell line may be distorted by a cross contamination of one cell line by another (Ribeiro et al, 2008; Schweppe et al, 2008), an in vitro evolution of the cell line (van Staveren et al, 2009), a strong genomic instability due to the number of passages or a risk of infection (Harlin and Gajewski, 2008). Thus, a systematic verification of the status of these cell lines is important (van Staveren et al, 2007; Ribeiro et al, 2008; Schweppe et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Thyroid cancer cell lines: an overview

et al. 2012

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Human thyroid cancer cell lines are the most used models for thyroid cancer studies. They must be used with detailed knowledge of their characteristics. These in vitro cell lines originate from differentiated and dedifferentiated in vivo human thyroid tumors. However, it has been shown that mRNA expression profiles of these cell lines were closer to dedifferentiated in vivo thyroid tumors (anaplastic thyroid carcinoma, ATC) than to differentiated ones. Here an overview of the knowledge of these models was made. The mutational status of six human thyroid cancer cell lines (WRO, FTC133, BCPAP, TPC1, K1, and 8505C) was in line with previously reported findings for 10 genes frequently mutated in thyroid cancer. However, the presence of a BRAF mutation (T1799A: V600E) in WRO questions the use of this cell line as a model for follicular thyroid carcinoma (FTC). Next, to investigate the biological meaning of the modulated mRNAs in these cells, a pathway analysis on previously obtained mRNA profiles was performed on five cell lines. In five cell lines, the MHC class II pathway was down-regulated and in four of them, ribosome biosynthesis and translation pathways were up-regulated. mRNA expression profiles of the cell lines were also compared to those of the different types of thyroid cancers. Three datasets originating from different microarray platforms and derived from distinct laboratories were used. This meta-analysis showed a significant higher correlation between the profiles of the thyroid cancer cell lines and ATC, than to differentiated thyroid tumors (i.e., PTC or FTC) specifically for DNA replication. This already observed higher correlation was obtained here with an increased number of in vivo tumors and using different platforms. In summary, this would suggest that some papillary thyroid carcinoma or follicular thyroid carcinoma (PTC or FTC) cell lines (i.e., TPC-1) might have partially lost their original DNA synthesis/replication regulation mechanisms during their in vitro cell adaptation/evolution.

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“…The most common sources of mycoplasma contamination in the laboratory are infected cells sent from another lab and laboratory personnel infected with Mycoplasma orale or Mycoplasma fermentas . Thus, any strains received from another laboratory should be screened for the presence of mycoplasma ( APPENDIX here; Harlin and Gajewski, ). Should Mycoplasma be detected, the best practice is to discard the contaminated flask in order to reduce the spread of contamination.…”

Section: Commentarymentioning

confidence: 99%

Toxoplasma gondii: Laboratory Maintenance and Growth

Khan

Grigg

2017

CP Microbiology

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Toxoplasma gondii is a highly successful Apicomplexan protozoan capable of infecting any warm-blooded animal worldwide. In humans, Toxoplasma infections are life-long with approximately one-third of the world’s population chronically infected. Although normally controlled by the host immune system, T. gondii infection can lead to a variety of clinical outcomes in individuals with immature or suppressed immune systems. After penetrating the intestine, parasites rapidly disseminate throughout the body and stimulate production of the cytokines interleukin (IL)-12, IL-18, and interferon (IFN)-γ by immune cells. These cytokines play a key role in host resistance to T. gondii by promoting a strong Th1 response. Recent reports show that gut commensal bacteria can act as molecular adjuvants during T. gondii infection. Thus, T. gondii is an excellent model system to study host-pathogen interactions. This unit outlines the protocols for in vitro and in vivo maintenance and growth of T. gondii.

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Diagnosis and Treatment of Mycoplasma‐Contaminated Cell Cultures

Cited by 10 publications

References 9 publications

Guidelines for the welfare and use of animals in cancer research

Guidelines for the welfare and use of animals in cancer research

Thyroid cancer cell lines: an overview

Toxoplasma gondii: Laboratory Maintenance and Growth

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